(Linear) (Six-base) MAPSORT of: KIEE1694.seq  Check: 3168  from: 1  to: 603



KIEE1694.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:45  ..

AflIII A'CryG_T

Cuts at:      0     317     603 
   Size:        317     286
 
BamHI G'GATC_C

Cuts at:      0      43      94     603 
   Size:         43      51     509

  Fragments arranged by size: 

                 509      51      43
 
BanII G_rGCy'C

Cuts at:      0     571     603 
   Size:        571      32
 
BbvCI CC'TCA_GC

Cuts at:      0     395     603 
   Size:        395     208
 
BclI T'GATC_A

Cuts at:      0      26     603 
   Size:         26     577
 
BlpI GC'TnA_GC

Cuts at:      0     272     570     603 
   Size:        272     298      33

  Fragments arranged by size: 

                 298     272      33
 
BmgBI CAC'GTC

Cuts at:      0     291     603 
   Size:        291     312
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     583     603 
   Size:        583      20
 
Bpu10I CC'TnA_GC

Cuts at:      0     395     603 
   Size:        395     208
 
BsaAI yAC'GTr

Cuts at:      0     161     318     603 
   Size:        161     157     285

  Fragments arranged by size: 

                 285     161     157
 
BsaHI Gr'CG_yC

Cuts at:      0     305     603 
   Size:        305     298
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     409     603 
   Size:        409     194
 
BseYI C'CCAG_C

Cuts at:      0      65     336     540     603 
   Size:         65     271     204      63

  Fragments arranged by size: 

                 271     204      65      63
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     262     342     603 
   Size:        262      80     261

  Fragments arranged by size: 

                 262     261      80
 
BsiHKAI G_wGCw'C

Cuts at:      0      54     571     603 
   Size:         54     517      32

  Fragments arranged by size: 

                 517      54      32
 
Bsp1286I G_dGCh'C

Cuts at:      0      54     571     603 
   Size:         54     517      32

  Fragments arranged by size: 

                 517      54      32
 
BsrFI r'CCGG_y

Cuts at:      0     528     603 
   Size:        528      75
 
BstYI r'GATC_y

Cuts at:      0      43      94     603 
   Size:         43      51     509

  Fragments arranged by size: 

                 509      51      43
 
BtgI C'CryG_G

Cuts at:      0     464     603 
   Size:        464     139
 
DraIII CAC_nnn'GTG

Cuts at:      0     562     603 
   Size:        562      41
 
EaeI y'GGCC_r

Cuts at:      0     143     461     603 
   Size:        143     318     142

  Fragments arranged by size: 

                 318     143     142
 
EcoICRI GAG'CTC

Cuts at:      0     569     603 
   Size:        569      34
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     398     603 
   Size:        398     205
 
HaeII r_GCGC'y

Cuts at:      0     547     603 
   Size:        547      56
 
MscI TGG'CCA

Cuts at:      0     145     603 
   Size:        145     458
 
MslI CAynn'nnrTG

Cuts at:      0     316     558     603 
   Size:        316     242      45

  Fragments arranged by size: 

                 316     242      45
 
MspA1I CmG'CkG

Cuts at:      0     525     603 
   Size:        525      78
 
NaeI GCC'GGC

Cuts at:      0     530     603 
   Size:        530      73
 
NgoMIV G'CCGG_C

Cuts at:      0     528     603 
   Size:        528      75
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     562     603 
   Size:        562      41
 
PfoI T'CCnGG_A

Cuts at:      0      39     284     603 
   Size:         39     245     319

  Fragments arranged by size: 

                 319     245      39
 
PmlI CAC'GTG

Cuts at:      0     161     603 
   Size:        161     442
 
PvuII CAG'CTG

Cuts at:      0     525     603 
   Size:        525      78
 
SacI G_AGCT'C

Cuts at:      0     571     603 
   Size:        571      32
 
StyI C'CwwG_G

Cuts at:      0     132     342     603 
   Size:        132     210     261

  Fragments arranged by size: 

                 261     210     132
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      48     603 
   Size:         48     555
 

 Enzymes that do cut:

   AflIII    BamHI    BanII    BbvCI     BclI     BlpI    BmgBI     BmrI
   Bpu10I    BsaAI    BsaHI    BseRI    BseYI     BsgI  BsiHKAI Bsp1286I
    BsrFI    BstYI     BtgI   DraIII     EaeI  EcoICRI    EcoNI    HaeII
     MscI     MslI   MspA1I     NaeI   NgoMIV    PflMI     PfoI     PmlI
    PvuII     SacI     StyI    TaqII

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI     BanI
     BbeI     BbsI     BcgI    BciVI    BfrBI     BglI    BglII Bme1580I
     BmtI     BplI     BpmI    BpuEI     BsaI    BsaBI    BsaWI    BsaXI
    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI
  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI     DraI     DrdI
     EagI     EarI     EciI  Eco57MI EcoO109I    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI    Hin4I   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MmeI     NarI     NcoI     NdeI     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI     PmeI
     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI
     PvuI    RsrII    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI     TatI
     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI