(Linear) (Six-base) MAPSORT of: KIEE1690.seq  Check: 6335  from: 1  to: 600



KIEE1690.orf

 With 182 enzymes: SgfI * 

                        July 3, 2012 16:59  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     273     600 
   Size:        273     327
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      66      98     600 
   Size:         66      32     502

  Fragments arranged by size: 

                 502      66      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     129     600 
   Size:        129     471
 
AseI AT'TA_AT

Cuts at:      0     431     600 
   Size:        431     169
 
BamHI G'GATC_C

Cuts at:      0     554     600 
   Size:        554      46
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     281     600 
   Size:        281     319
 
BclI T'GATC_A

Cuts at:      0     183     600 
   Size:        183     417
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     560     600 
   Size:        560      40
 
BmtI G_CTAG'C

Cuts at:      0      70     600 
   Size:         70     530
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     454     600 
   Size:        454     146
 
BsaAI yAC'GTr

Cuts at:      0     565     600 
   Size:        565      35
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     385     600 
   Size:        385     215
 
BseYI C'CCAG_C

Cuts at:      0     558     600 
   Size:        558      42
 
BsmI GAATG_Cn'

Cuts at:      0      15     600 
   Size:         15     585
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     273     600 
   Size:        273     327
 
BsrBI CCG'CTC

Cuts at:      0      56     600 
   Size:         56     544
 
BsrDI GCAATG_nn'

Cuts at:      0     414     600 
   Size:        414     186
 
BstYI r'GATC_y

Cuts at:      0     398     554     600 
   Size:        398     156      46

  Fragments arranged by size: 

                 398     156      46
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     510     600 
   Size:        510      90
 
EaeI y'GGCC_r

Cuts at:      0     260     600 
   Size:        260     340
 
EcoO109I rG'GnC_Cy

Cuts at:      0      42     126     471     600 
   Size:         42      84     345     129

  Fragments arranged by size: 

                 345     129      84      42
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     244     600 
   Size:        244     356
 
HindIII A'AGCT_T

Cuts at:      0     233     600 
   Size:        233     367
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     417     600 
   Size:        417     183
 
MscI TGG'CCA

Cuts at:      0     262     600 
   Size:        262     338
 
MspA1I CmG'CkG

Cuts at:      0      24     600 
   Size:         24     576
 
NheI G'CTAG_C

Cuts at:      0      66     600 
   Size:         66     534
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     565     600 
   Size:        565      35
 
PfoI T'CCnGG_A

Cuts at:      0     386     600 
   Size:        386     214
 
PmlI CAC'GTG

Cuts at:      0     565     600 
   Size:        565      35
 
PpuMI rG'GwC_Cy

Cuts at:      0      42     600 
   Size:         42     558
 
PstI C_TGCA'G

Cuts at:      0     488     600 
   Size:        488     112
 
SfcI C'TryA_G

Cuts at:      0      48     484     600 
   Size:         48     436     116

  Fragments arranged by size: 

                 436     116      48
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     217     600 
   Size:        217     383
 

 Enzymes that do cut:

     AarI     AloI    AlwNI     AseI    BamHI     BbsI     BclI     BmrI
     BmtI     BsaI    BsaAI    BseRI    BseYI     BsmI    BspMI    BsrBI
    BsrDI    BstYI     DrdI     EaeI EcoO109I     FalI  HindIII     MmeI
     MscI   MspA1I     NheI    PflMI     PfoI     PmlI    PpuMI     PstI
     SfcI    TaqII

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     ApaI    ApaLI     ApoI     AscI
    AsiSI     AvaI    AvrII     BaeI     BanI    BanII     BbeI    BbvCI
     BcgI    BciVI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI
     BplI     BpmI   Bpu10I    BpuEI    BsaBI    BsaHI    BsaWI    BsaXI
     BsgI    BsiEI  BsiHKAI    BsiWI    BsmBI Bsp1286I    BspEI    BspHI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI
  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI
   DraIII     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI    EcoRI
    EcoRV     FseI     FspI    FspAI    HaeII    Hin4I   HincII     HpaI
     KasI     KpnI     MfeI     MluI     MslI     NaeI     NarI     NcoI
     NdeI   NgoMIV     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI     PmeI     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI
     SwaI     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI
     XmnI     ZraI