(Linear) (Six-base) MAPSORT of: KIEE1688.seq  Check: 8918  from: 1  to: 597



KIEE1688.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:45  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     436     597 
   Size:        436     161
 
AflIII A'CryG_T

Cuts at:      0     539     597 
   Size:        539      58
 
AleI CACnn'nnGTG

Cuts at:      0     586     597 
   Size:        586      11
 
AlwNI CAG_nnn'CTG

Cuts at:      0     165     597 
   Size:        165     432
 
ApaLI G'TGCA_C

Cuts at:      0     517     597 
   Size:        517      80
 
ApoI r'AATT_y

Cuts at:      0     116     241     597 
   Size:        116     125     356

  Fragments arranged by size: 

                 356     125     116
 
BfrBI ATG'CAT

Cuts at:      0     473     597 
   Size:        473     124
 
Bme1580I G_kGCm'C

Cuts at:      0     521     597 
   Size:        521      76
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     338     597 
   Size:        338     259
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     461     597 
   Size:        461     136
 
BsiHKAI G_wGCw'C

Cuts at:      0     521     597 
   Size:        521      76
 
Bsp1286I G_dGCh'C

Cuts at:      0     521     597 
   Size:        521      76
 
BspHI T'CATG_A

Cuts at:      0     545     597 
   Size:        545      52
 
BsrGI T'GTAC_A

Cuts at:      0     111     597 
   Size:        111     486
 
BstEII G'GTnAC_C

Cuts at:      0     425     597 
   Size:        425     172
 
BtsI GCAGTG_nn'

Cuts at:      0     560     597 
   Size:        560      37
 
DraI TTT'AAA

Cuts at:      0     153     357     597 
   Size:        153     204     240

  Fragments arranged by size: 

                 240     204     153
 
EarI CTCTTCn'nnn_

Cuts at:      0      34     597 
   Size:         34     563
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     436     597 
   Size:        436     161
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     382     597 
   Size:        382     215
 
FspI TGC'GCA

Cuts at:      0      40     597 
   Size:         40     557
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     287     597 
   Size:        287     310
 
MslI CAynn'nnrTG

Cuts at:      0     305     389     544     586     597 
   Size:        305      84     155      42      11

  Fragments arranged by size: 

                 305     155      84      42      11
 
MspA1I CmG'CkG

Cuts at:      0     414     597 
   Size:        414     183
 
NsiI A_TGCA'T

Cuts at:      0     475     597 
   Size:        475     122
 
NspI r_CATG'y

Cuts at:      0     543     597 
   Size:        543      54
 
PciI A'CATG_T

Cuts at:      0     539     597 
   Size:        539      58
 
PvuII CAG'CTG

Cuts at:      0     414     597 
   Size:        414     183
 
StuI AGG'CCT

Cuts at:      0      11     597 
   Size:         11     586
 
TatI w'GTAC_w

Cuts at:      0     111     597 
   Size:        111     486
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     206     238     597 
   Size:        206      32     359

  Fragments arranged by size: 

                 359     206      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     139     597 
   Size:        139     458
 
XbaI T'CTAG_A

Cuts at:      0     574     597 
   Size:        574      23
 

 Enzymes that do cut:

     AcuI   AflIII     AleI    AlwNI    ApaLI     ApoI    BfrBI Bme1580I
    BseRI     BsgI  BsiHKAI Bsp1286I    BspHI    BsrGI   BstEII     BtsI
     DraI     EarI  Eco57MI     FalI     FspI     MmeI     MslI   MspA1I
     NsiI     NspI     PciI    PvuII     StuI     TatI     TstI  Tth111I
     XbaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AlfI     AloI     ApaI     AscI     AseI    AsiSI     AvaI
    AvrII     BaeI    BamHI     BanI    BanII     BbeI     BbsI    BbvCI
     BcgI    BciVI     BclI     BglI    BglII     BlpI    BmgBI     BmrI
     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseYI    BsiEI    BsiWI     BsmI    BsmBI
    BspEI    BspMI    BsrBI    BsrDI    BsrFI   BssHII    BssSI   BstAPI
    BstBI    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI
    CspCI   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI    EcoNI
 EcoO109I    EcoRI    EcoRV     FseI    FspAI    HaeII    Hin4I   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MscI     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     PacI
     PasI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     StyI     SwaI    TaqII     XcmI     XhoI
     XmaI     XmnI     ZraI