Restriction map for
pFN21AE1684
(Linear) (Six-base) MAPSORT of: KIEE1684.seq Check: 3854 from: 1 to: 588
KIEE1684.orf
With 182 enzymes: SgfI *
July 3, 2012 16:58 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 246 588
Size: 246 342
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 217 325 588
Size: 217 108 263
Fragments arranged by size:
263 217 108
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 37 123 588
Size: 37 86 465
Fragments arranged by size:
465 86 37
AlwNI CAG_nnn'CTG
Cuts at: 0 67 147 236 588
Size: 67 80 89 352
Fragments arranged by size:
352 89 80 67
ApaI G_GGCC'C
Cuts at: 0 434 588
Size: 434 154
AvaI C'yCGr_G
Cuts at: 0 440 508 588
Size: 440 68 80
Fragments arranged by size:
440 80 68
BanII G_rGCy'C
Cuts at: 0 90 434 588
Size: 90 344 154
Fragments arranged by size:
344 154 90
BbvCI CC'TCA_GC
Cuts at: 0 416 588
Size: 416 172
BciVI GTATCCnnnnn_n'
Cuts at: 0 418 588
Size: 418 170
BlpI GC'TnA_GC
Cuts at: 0 287 588
Size: 287 301
Bme1580I G_kGCm'C
Cuts at: 0 434 588
Size: 434 154
BmrI ACTGGGnnnn_n'
Cuts at: 0 114 588
Size: 114 474
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 77 334 457 588
Size: 77 257 123 131
Fragments arranged by size:
257 131 123 77
Bpu10I CC'TnA_GC
Cuts at: 0 416 588
Size: 416 172
BsaI GGTCTCn'nnnn_
Cuts at: 0 545 588
Size: 545 43
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 532 588
Size: 532 56
BseYI C'CCAG_C
Cuts at: 0 336 392 588
Size: 336 56 196
Fragments arranged by size:
336 196 56
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 361 448 588
Size: 361 87 140
Fragments arranged by size:
361 140 87
Bsp1286I G_dGCh'C
Cuts at: 0 90 434 588
Size: 90 344 154
Fragments arranged by size:
344 154 90
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 246 588
Size: 246 342
BsrBI CCG'CTC
Cuts at: 0 215 588
Size: 215 373
BsrFI r'CCGG_y
Cuts at: 0 467 588
Size: 467 121
Bsu36I CC'TnA_GG
Cuts at: 0 244 588
Size: 244 344
BtgI C'CryG_G
Cuts at: 0 81 272 422 588
Size: 81 191 150 166
Fragments arranged by size:
191 166 150 81
BtsI GCAGTG_nn'
Cuts at: 0 47 588
Size: 47 541
DrdI GACnn_nn'nnGTC
Cuts at: 0 583 588
Size: 583 5
EaeI y'GGCC_r
Cuts at: 0 35 588
Size: 35 553
EarI CTCTTCn'nnn_
Cuts at: 0 176 229 538 588
Size: 176 53 309 50
Fragments arranged by size:
309 176 53 50
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 526 588
Size: 526 62
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 77 217 325 334 457 588
Size: 77 140 108 9 123 131
Fragments arranged by size:
140 131 123 108 77 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 159 588
Size: 159 429
EcoO109I rG'GnC_Cy
Cuts at: 0 233 240 430 437 480 576 588
Size: 233 7 190 7 43 96 12
Fragments arranged by size:
233 190 96 43 12 7 7
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 205 588
Size: 205 383
HaeII r_GCGC'y
Cuts at: 0 270 588
Size: 270 318
MslI CAynn'nnrTG
Cuts at: 0 19 588
Size: 19 569
MspA1I CmG'CkG
Cuts at: 0 83 252 325 572 588
Size: 83 169 73 247 16
Fragments arranged by size:
247 169 83 73 16
NspI r_CATG'y
Cuts at: 0 18 588
Size: 18 570
PasI CC'CwG_GG
Cuts at: 0 342 588
Size: 342 246
PflMI CCAn_nnn'nTGG
Cuts at: 0 236 588
Size: 236 352
PfoI T'CCnGG_A
Cuts at: 0 92 473 588
Size: 92 381 115
Fragments arranged by size:
381 115 92
PpuMI rG'GwC_Cy
Cuts at: 0 233 240 437 576 588
Size: 233 7 197 139 12
Fragments arranged by size:
233 197 139 12 7
PspOMI G'GGCC_C
Cuts at: 0 430 588
Size: 430 158
PspXI vC'TCGA_Gb
Cuts at: 0 508 588
Size: 508 80
PstI C_TGCA'G
Cuts at: 0 152 257 588
Size: 152 105 331
Fragments arranged by size:
331 152 105
PvuII CAG'CTG
Cuts at: 0 252 588
Size: 252 336
SacII CC_GC'GG
Cuts at: 0 84 588
Size: 84 504
SfcI C'TryA_G
Cuts at: 0 148 253 588
Size: 148 105 335
Fragments arranged by size:
335 148 105
SmaI CCC'GGG
Cuts at: 0 442 588
Size: 442 146
SmlI C'TyrA_G
Cuts at: 0 508 588
Size: 508 80
SphI G_CATG'C
Cuts at: 0 18 588
Size: 18 570
StuI AGG'CCT
Cuts at: 0 154 514 588
Size: 154 360 74
Fragments arranged by size:
360 154 74
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 193 225 588
Size: 193 32 363
Fragments arranged by size:
363 193 32
XhoI C'TCGA_G
Cuts at: 0 508 588
Size: 508 80
XmaI C'CCGG_G
Cuts at: 0 440 588
Size: 440 148
Enzymes that do cut:
AarI AcuI AlfI AlwNI ApaI AvaI BanII BbvCI
BciVI BlpI Bme1580I BmrI BpmI Bpu10I BsaI BseRI
BseYI BsgI Bsp1286I BspMI BsrBI BsrFI Bsu36I BtgI
BtsI DrdI EaeI EarI EciI Eco57MI EcoNI EcoO109I
FalI HaeII MslI MspA1I NspI PasI PflMI PfoI
PpuMI PspOMI PspXI PstI PvuII SacII SfcI SmaI
SmlI SphI StuI TstI XhoI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AloI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BanI BbeI BbsI BcgI BclI
BfrBI BglI BglII BmgBI BmtI BplI BpuEI BsaAI
BsaBI BsaHI BsaWI BsaXI BsiEI BsiHKAI BsiWI BsmI
BsmBI BspEI BspHI BsrDI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BstZ17I BtgZI ClaI CspCI
DraI DraIII EagI EcoICRI EcoRI EcoRV FseI FspI
FspAI Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI PacI PciI PmeI PmlI
PpiI PshAI PsiI PsrI PvuI RsrII SacI SalI
SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SnaBI SpeI SrfI SspI StyI SwaI TaqII
TatI Tth111I XbaI XcmI XmnI ZraI