Restriction map for
pFN21AE1682
(Linear) (Six-base) MAPSORT of: KIEE1682.seq Check: 1714 from: 1 to: 588
KIEE1682.orf
With 182 enzymes: SgfI *
July 15, 2010 18:45 ..
AccI GT'mk_AC
Cuts at: 0 314 450 588
Size: 314 136 138
Fragments arranged by size:
314 138 136
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 150 588
Size: 150 438
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 358 390 588
Size: 358 32 198
Fragments arranged by size:
358 198 32
AlwNI CAG_nnn'CTG
Cuts at: 0 147 197 588
Size: 147 50 391
Fragments arranged by size:
391 147 50
BanI G'GyrC_C
Cuts at: 0 343 414 588
Size: 343 71 174
Fragments arranged by size:
343 174 71
BanII G_rGCy'C
Cuts at: 0 33 311 480 588
Size: 33 278 169 108
Fragments arranged by size:
278 169 108 33
BbsI GAAGACnn'nnnn_
Cuts at: 0 80 262 308 588
Size: 80 182 46 280
Fragments arranged by size:
280 182 80 46
BglI GCCn_nnn'nGGC
Cuts at: 0 436 588
Size: 436 152
Bme1580I G_kGCm'C
Cuts at: 0 104 348 419 588
Size: 104 244 71 169
Fragments arranged by size:
244 169 104 71
BmgBI CAC'GTC
Cuts at: 0 276 588
Size: 276 312
BmrI ACTGGGnnnn_n'
Cuts at: 0 271 588
Size: 271 317
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 518 588
Size: 518 70
Bpu10I CC'TnA_GC
Cuts at: 0 62 148 470 588
Size: 62 86 322 118
Fragments arranged by size:
322 118 86 62
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 516 546 565 588
Size: 516 30 19 23
Fragments arranged by size:
516 30 23 19
BseYI C'CCAG_C
Cuts at: 0 37 140 588
Size: 37 103 448
Fragments arranged by size:
448 103 37
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 259 538 588
Size: 259 279 50
Fragments arranged by size:
279 259 50
BsiHKAI G_wGCw'C
Cuts at: 0 500 588
Size: 500 88
Bsp1286I G_dGCh'C
Cuts at: 0 33 104 311 348 419 480 500 588
Size: 33 71 207 37 71 61 20 88
Fragments arranged by size:
207 88 71 71 61 37 33 20
BsrBI CCG'CTC
Cuts at: 0 11 58 581 588
Size: 11 47 523 7
Fragments arranged by size:
523 47 11 7
BssSI C'ACGA_G
Cuts at: 0 213 588
Size: 213 375
BstAPI GCAn_nnn'nTGC
Cuts at: 0 197 588
Size: 197 391
BstZ17I GTA'TAC
Cuts at: 0 315 588
Size: 315 273
Bsu36I CC'TnA_GG
Cuts at: 0 481 588
Size: 481 107
BtgI C'CryG_G
Cuts at: 0 324 588
Size: 324 264
EaeI y'GGCC_r
Cuts at: 0 568 588
Size: 568 20
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 518 588
Size: 518 70
EcoO109I rG'GnC_Cy
Cuts at: 0 120 588
Size: 120 468
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 512 588
Size: 512 76
MscI TGG'CCA
Cuts at: 0 570 588
Size: 570 18
MslI CAynn'nnrTG
Cuts at: 0 132 512 588
Size: 132 380 76
Fragments arranged by size:
380 132 76
MspA1I CmG'CkG
Cuts at: 0 197 588
Size: 197 391
PasI CC'CwG_GG
Cuts at: 0 444 588
Size: 444 144
PstI C_TGCA'G
Cuts at: 0 91 588
Size: 91 497
PvuII CAG'CTG
Cuts at: 0 197 588
Size: 197 391
SfcI C'TryA_G
Cuts at: 0 87 588
Size: 87 501
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 565 588
Size: 565 23
Enzymes that do cut:
AccI AlfI AloI AlwNI BanI BanII BbsI BglI
Bme1580I BmgBI BmrI BpmI Bpu10I BsaXI BseYI BsgI
BsiHKAI Bsp1286I BsrBI BssSI BstAPI BstZ17I Bsu36I BtgI
EaeI Eco57MI EcoO109I MmeI MscI MslI MspA1I PasI
PstI PvuII SfcI TstI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI ApaI ApaLI ApoI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BbeI BbvCI BcgI
BciVI BclI BfrBI BglII BlpI BmtI BplI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BsiEI BsiWI
BsmI BsmBI BspEI BspHI BspMI BsrDI BsrFI BsrGI
BssHII BstBI BstEII BstXI BstYI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EagI EarI EciI EcoICRI
EcoNI EcoRI EcoRV FalI FseI FspI FspAI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI StuI StyI SwaI TaqII
TatI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI