(Linear) (Six-base) MAPSORT of: KIEE1681.seq  Check: 6157  from: 1  to: 582



KIEE1681.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:45  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     267     582 
   Size:        267     315
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     458     490     582 
   Size:        458      32      92

  Fragments arranged by size: 

                 458      92      32
 
BbvCI CC'TCA_GC

Cuts at:      0      31     514     582 
   Size:         31     483      68

  Fragments arranged by size: 

                 483      68      31
 
BlpI GC'TnA_GC

Cuts at:      0     182     244     582 
   Size:        182      62     338

  Fragments arranged by size: 

                 338     182      62
 
Bme1580I G_kGCm'C

Cuts at:      0      63     582 
   Size:         63     519
 
BmgBI CAC'GTC

Cuts at:      0      66     486     582 
   Size:         66     420      96

  Fragments arranged by size: 

                 420      96      66
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     160     582 
   Size:        160     422
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     427     537     582 
   Size:        427     110      45

  Fragments arranged by size: 

                 427     110      45
 
Bpu10I CC'TnA_GC

Cuts at:      0      31     514     582 
   Size:         31     483      68

  Fragments arranged by size: 

                 483      68      31
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     363     393     582 
   Size:        363      30     189

  Fragments arranged by size: 

                 363     189      30
 
BseYI C'CCAG_C

Cuts at:      0     158     174     300     497     508     582 
   Size:        158      16     126     197      11      74

  Fragments arranged by size: 

                 197     158     126      74      16      11
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     412     582 
   Size:        412     170
 
BsiHKAI G_wGCw'C

Cuts at:      0     326     355     376     455     582 
   Size:        326      29      21      79     127

  Fragments arranged by size: 

                 326     127      79      29      21
 
Bsp1286I G_dGCh'C

Cuts at:      0      63     326     355     376     455     582 
   Size:         63     263      29      21      79     127

  Fragments arranged by size: 

                 263     127      79      63      29      21
 
BsrDI GCAATG_nn'

Cuts at:      0     312     582 
   Size:        312     270
 
BssSI C'ACGA_G

Cuts at:      0     454     582 
   Size:        454     128
 
BtsI GCAGTG_nn'

Cuts at:      0     119     193     582 
   Size:        119      74     389

  Fragments arranged by size: 

                 389     119      74
 
EaeI y'GGCC_r

Cuts at:      0     211     582 
   Size:        211     371
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     267     427     537     582 
   Size:        267     160     110      45

  Fragments arranged by size: 

                 267     160     110      45
 
EcoO109I rG'GnC_Cy

Cuts at:      0      45     144     582 
   Size:         45      99     438

  Fragments arranged by size: 

                 438      99      45
 
MscI TGG'CCA

Cuts at:      0     213     582 
   Size:        213     369
 
MspA1I CmG'CkG

Cuts at:      0     115     387     414     582 
   Size:        115     272      27     168

  Fragments arranged by size: 

                 272     168     115      27
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     235     582 
   Size:        235     347
 
PpuMI rG'GwC_Cy

Cuts at:      0      45     144     582 
   Size:         45      99     438

  Fragments arranged by size: 

                 438      99      45
 
PstI C_TGCA'G

Cuts at:      0     268     582 
   Size:        268     314
 
PvuII CAG'CTG

Cuts at:      0     115     387     414     582 
   Size:        115     272      27     168

  Fragments arranged by size: 

                 272     168     115      27
 
SfcI C'TryA_G

Cuts at:      0     264     582 
   Size:        264     318
 
StuI AGG'CCT

Cuts at:      0     397     582 
   Size:        397     185
 
StyI C'CwwG_G

Cuts at:      0     398     582 
   Size:        398     184
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     550     582 
   Size:        550      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     338     505     582 
   Size:        338     167      77

  Fragments arranged by size: 

                 338     167      77
 

 Enzymes that do cut:

     AcuI     AloI    BbvCI     BlpI Bme1580I    BmgBI     BmrI     BpmI
   Bpu10I    BsaXI    BseYI     BsgI  BsiHKAI Bsp1286I    BsrDI    BssSI
     BtsI     EaeI  Eco57MI EcoO109I     MscI   MspA1I    PflMI    PpuMI
     PstI    PvuII     SfcI     StuI     StyI    TaqII     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI    AlwNI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI     BbsI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII     BmtI     BplI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BseRI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI
    BspMI    BsrBI    BsrFI    BsrGI   BssHII   BstAPI    BstBI   BstEII
    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI    CspCI
     DraI   DraIII     DrdI     EagI     EarI     EciI  EcoICRI    EcoNI
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII    Hin4I
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI
     MslI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     NspI     PacI     PasI     PciI     PfoI     PmeI
     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     SspI     SwaI     TatI     TstI  Tth111I
     XbaI     XhoI     XmaI     XmnI     ZraI