(Linear) (Six-base) MAPSORT of: KIEE1677.seq  Check: 7210  from: 1  to: 579



KIEE1677.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:44  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     101     579 
   Size:        101     478
 
ApoI r'AATT_y

Cuts at:      0      49     147     317     579 
   Size:         49      98     170     262

  Fragments arranged by size: 

                 262     170      98      49
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      26     579 
   Size:         26     553
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     381     466     579 
   Size:        381      85     113

  Fragments arranged by size: 

                 381     113      85
 
BglII A'GATC_T

Cuts at:      0     547     579 
   Size:        547      32
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     494     579 
   Size:        494      85
 
BsrDI GCAATG_nn'

Cuts at:      0      50     579 
   Size:         50     529
 
BstYI r'GATC_y

Cuts at:      0     547     579 
   Size:        547      32
 
DraI TTT'AAA

Cuts at:      0     510     579 
   Size:        510      69
 
EarI CTCTTCn'nnn_

Cuts at:      0     172     579 
   Size:        172     407
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     101     579 
   Size:        101     478
 
HindIII A'AGCT_T

Cuts at:      0     118     579 
   Size:        118     461
 
MfeI C'AATT_G

Cuts at:      0      24     579 
   Size:         24     555
 
PsiI TTA'TAA

Cuts at:      0     253     579 
   Size:        253     326
 
SfcI C'TryA_G

Cuts at:      0     219     579 
   Size:        219     360
 
StyI C'CwwG_G

Cuts at:      0     177     579 
   Size:        177     402
 

 Enzymes that do cut:

     AcuI     ApoI     BbsI    BciVI    BglII     BsaI    BsrDI    BstYI
     DraI     EarI  Eco57MI  HindIII     MfeI     PsiI     SfcI     StyI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI    BbvCI     BcgI     BclI    BfrBI     BglI     BlpI
 Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI
    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI
   BstEII    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI
    CspCI   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI    EcoNI
 EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII
    Hin4I   HincII     HpaI     KasI     KpnI     MluI     MmeI     MscI
     MslI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI
     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI   PspOMI    PspXI
     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     SwaI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI
     XhoI     XmaI     XmnI     ZraI