(Linear) (Six-base) MAPSORT of: KIEE1667.seq  Check: 33  from: 1  to: 561



KIEE1667.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:44  ..

AvaI C'yCGr_G

Cuts at:      0     122     264     561 
   Size:        122     142     297

  Fragments arranged by size: 

                 297     142     122
 
BanI G'GyrC_C

Cuts at:      0      53     561 
   Size:         53     508
 
BanII G_rGCy'C

Cuts at:      0     242     561 
   Size:        242     319
 
BbeI G_GCGC'C

Cuts at:      0      57     561 
   Size:         57     504
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     239     561 
   Size:        239     322
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      96     212     561 
   Size:         96     116     349

  Fragments arranged by size: 

                 349     116      96
 
BsaHI Gr'CG_yC

Cuts at:      0      54     561 
   Size:         54     507
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     142     172     229     259     561 
   Size:        142      30      57      30     302

  Fragments arranged by size: 

                 302     142      57      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     163     561 
   Size:        163     398
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      79     561 
   Size:         79     482
 
Bsp1286I G_dGCh'C

Cuts at:      0     242     561 
   Size:        242     319
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     288     561 
   Size:        288     273
 
BsrBI CCG'CTC

Cuts at:      0     119     561 
   Size:        119     442
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     487     561 
   Size:        487      74
 
BtsI GCAGTG_nn'

Cuts at:      0      91     519     561 
   Size:         91     428      42

  Fragments arranged by size: 

                 428      91      42
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     160     561 
   Size:        160     401
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      96     212     561 
   Size:         96     116     349

  Fragments arranged by size: 

                 349     116      96
 
HaeII r_GCGC'y

Cuts at:      0      57     561 
   Size:         57     504
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     142     174     229     261     561 
   Size:        142      32      55      32     300

  Fragments arranged by size: 

                 300     142      55      32      32
 
KasI G'GCGC_C

Cuts at:      0      53     561 
   Size:         53     508
 
MfeI C'AATT_G

Cuts at:      0     182     561 
   Size:        182     379
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     468     561 
   Size:        468      93
 
MspA1I CmG'CkG

Cuts at:      0     253     561 
   Size:        253     308
 
NarI GG'CG_CC

Cuts at:      0      54     561 
   Size:         54     507
 
PstI C_TGCA'G

Cuts at:      0     258     561 
   Size:        258     303
 
SfcI C'TryA_G

Cuts at:      0     254     561 
   Size:        254     307
 
SfoI GGC'GCC

Cuts at:      0      55     561 
   Size:         55     506
 
SmaI CCC'GGG

Cuts at:      0     266     561 
   Size:        266     295
 
SmlI C'TyrA_G

Cuts at:      0     122     561 
   Size:        122     439
 
StyI C'CwwG_G

Cuts at:      0     384     561 
   Size:        384     177
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     365     397     561 
   Size:        365      32     164

  Fragments arranged by size: 

                 365     164      32
 
XhoI C'TCGA_G

Cuts at:      0     122     561 
   Size:        122     439
 
XmaI C'CCGG_G

Cuts at:      0     264     561 
   Size:        264     297
 

 Enzymes that do cut:

     AvaI     BanI    BanII     BbeI     BbsI     BpmI    BsaHI    BsaXI
    BseRI     BsgI Bsp1286I    BspMI    BsrBI    BstXI     BtsI     EciI
  Eco57MI    HaeII    Hin4I     KasI     MfeI     MmeI   MspA1I     NarI
     PstI     SfcI     SfoI     SmaI     SmlI     StyI     TstI     XhoI
     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     ApoI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BmtI     BplI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaBI    BsaWI    BseYI    BsiEI  BsiHKAI    BsiWI     BsmI
    BsmBI    BspEI    BspHI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI
     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EarI
  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI   HincII  HindIII     HpaI     KpnI     MluI     MscI     MslI
     NaeI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI
    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfiI     SgfI    SgrAI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     SwaI    TaqII     TatI  Tth111I     XbaI
     XcmI     XmnI     ZraI