(Linear) (Six-base) MAPSORT of: KIEE1663.seq  Check: 5698  from: 1  to: 549



KIEE1663.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:44  ..

Acc65I G'GTAC_C

Cuts at:      0     478     549 
   Size:        478      71
 
AleI CACnn'nnGTG

Cuts at:      0      40     549 
   Size:         40     509
 
AlwNI CAG_nnn'CTG

Cuts at:      0     253     549 
   Size:        253     296
 
ApoI r'AATT_y

Cuts at:      0      97     218     549 
   Size:         97     121     331

  Fragments arranged by size: 

                 331     121      97
 
AseI AT'TA_AT

Cuts at:      0     272     549 
   Size:        272     277
 
BanI G'GyrC_C

Cuts at:      0     478     549 
   Size:        478      71
 
BanII G_rGCy'C

Cuts at:      0     505     549 
   Size:        505      44
 
BmgBI CAC'GTC

Cuts at:      0      42     549 
   Size:         42     507
 
BmrI ACTGGGnnnn_n'

Cuts at:      0       5     549 
   Size:          5     544
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     466     549 
   Size:        466      83
 
BsiHKAI G_wGCw'C

Cuts at:      0     505     549 
   Size:        505      44
 
Bsp1286I G_dGCh'C

Cuts at:      0     505     549 
   Size:        505      44
 
BspHI T'CATG_A

Cuts at:      0     209     549 
   Size:        209     340
 
BstYI r'GATC_y

Cuts at:      0     357     549 
   Size:        357     192
 
BtgI C'CryG_G

Cuts at:      0     446     549 
   Size:        446     103
 
EarI CTCTTCn'nnn_

Cuts at:      0     467     549 
   Size:        467      82
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      42     158     549 
   Size:         42     116     391

  Fragments arranged by size: 

                 391     116      42
 
EcoICRI GAG'CTC

Cuts at:      0     503     549 
   Size:        503      46
 
HaeII r_GCGC'y

Cuts at:      0     460     549 
   Size:        460      89
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     229     261     478     510     549 
   Size:        229      32     217      32      39

  Fragments arranged by size: 

                 229     217      39      32      32
 
KpnI G_GTAC'C

Cuts at:      0     482     549 
   Size:        482      67
 
MslI CAynn'nnrTG

Cuts at:      0      40     549 
   Size:         40     509
 
NdeI CA'TA_TG

Cuts at:      0     135     549 
   Size:        135     414
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     540     549 
   Size:        540       9
 
PstI C_TGCA'G

Cuts at:      0     381     549 
   Size:        381     168
 
SacI G_AGCT'C

Cuts at:      0     505     549 
   Size:        505      44
 
SfcI C'TryA_G

Cuts at:      0     200     377     549 
   Size:        200     177     172

  Fragments arranged by size: 

                 200     177     172
 
TatI w'GTAC_w

Cuts at:      0     428     549 
   Size:        428     121
 
Tth111I GACn'n_nGTC

Cuts at:      0      43     549 
   Size:         43     506
 
XbaI T'CTAG_A

Cuts at:      0     261     549 
   Size:        261     288
 

 Enzymes that do cut:

   Acc65I     AleI    AlwNI     ApoI     AseI     BanI    BanII    BmgBI
     BmrI    BseRI  BsiHKAI Bsp1286I    BspHI    BstYI     BtgI     EarI
     EciI  EcoICRI    HaeII    Hin4I     KpnI     MslI     NdeI    PflMI
     PstI     SacI     SfcI     TatI  Tth111I     XbaI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AlfI     AloI     ApaI    ApaLI     AscI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BbeI     BbsI    BbvCI     BcgI
    BciVI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I     BmtI
     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseYI     BsgI    BsiEI    BsiWI     BsmI    BsmBI
    BspEI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I    BtgZI     BtsI
     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI  Eco57MI
    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
   HincII  HindIII     HpaI     KasI     MfeI     MluI     MmeI     MscI
   MspA1I     NaeI     NarI     NcoI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI
    PvuII    RsrII    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     StyI     SwaI    TaqII
     TstI     XcmI     XhoI     XmaI     XmnI     ZraI