Restriction map for
pFN21AE1662
(Linear) (Six-base) MAPSORT of: KIEE1662.seq Check: 2653 from: 1 to: 540
KIEE1662.orf
With 182 enzymes: SgfI *
July 15, 2010 18:44 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 538 540
Size: 538 2
AhdI GACnn_n'nnGTC
Cuts at: 0 147 540
Size: 147 393
ApaI G_GGCC'C
Cuts at: 0 110 540
Size: 110 430
BanI G'GyrC_C
Cuts at: 0 132 359 540
Size: 132 227 181
Fragments arranged by size:
227 181 132
BanII G_rGCy'C
Cuts at: 0 73 110 540
Size: 73 37 430
Fragments arranged by size:
430 73 37
BbvCI CC'TCA_GC
Cuts at: 0 217 540
Size: 217 323
BciVI GTATCCnnnnn_n'
Cuts at: 0 343 540
Size: 343 197
Bme1580I G_kGCm'C
Cuts at: 0 110 540
Size: 110 430
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 311 540
Size: 311 229
Bpu10I CC'TnA_GC
Cuts at: 0 217 540
Size: 217 323
BsaI GGTCTCn'nnnn_
Cuts at: 0 502 540
Size: 502 38
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 343 373 540
Size: 343 30 167
Fragments arranged by size:
343 167 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 193 214 300 540
Size: 193 21 86 240
Fragments arranged by size:
240 193 86 21
BseYI C'CCAG_C
Cuts at: 0 54 212 325 540
Size: 54 158 113 215
Fragments arranged by size:
215 158 113 54
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 510 540
Size: 510 30
Bsp1286I G_dGCh'C
Cuts at: 0 73 110 540
Size: 73 37 430
Fragments arranged by size:
430 73 37
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 26 353 538 540
Size: 26 327 185 2
Fragments arranged by size:
327 185 26 2
BsrFI r'CCGG_y
Cuts at: 0 423 540
Size: 423 117
BssHII G'CGCG_C
Cuts at: 0 495 540
Size: 495 45
BstXI CCAn_nnnn'nTGG
Cuts at: 0 48 519 540
Size: 48 471 21
Fragments arranged by size:
471 48 21
BtgI C'CryG_G
Cuts at: 0 338 540
Size: 338 202
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 498 540
Size: 498 42
DraIII CAC_nnn'GTG
Cuts at: 0 132 540
Size: 132 408
EcoNI CCTnn'n_nnAGG
Cuts at: 0 349 540
Size: 349 191
EcoO109I rG'GnC_Cy
Cuts at: 0 107 481 540
Size: 107 374 59
Fragments arranged by size:
374 107 59
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 133 165 540
Size: 133 32 375
Fragments arranged by size:
375 133 32
HincII GTy'rAC
Cuts at: 0 258 540
Size: 258 282
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 302 540
Size: 302 238
MslI CAynn'nnrTG
Cuts at: 0 517 540
Size: 517 23
MspA1I CmG'CkG
Cuts at: 0 216 267 540
Size: 216 51 273
Fragments arranged by size:
273 216 51
PasI CC'CwG_GG
Cuts at: 0 299 540
Size: 299 241
PspOMI G'GGCC_C
Cuts at: 0 106 540
Size: 106 434
PvuII CAG'CTG
Cuts at: 0 216 267 540
Size: 216 51 273
Fragments arranged by size:
273 216 51
SexAI A'CCwGG_T
Cuts at: 0 128 143 540
Size: 128 15 397
Fragments arranged by size:
397 128 15
SfcI C'TryA_G
Cuts at: 0 306 540
Size: 306 234
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 343 375 540
Size: 343 32 165
Fragments arranged by size:
343 165 32
XmnI GAAnn'nnTTC
Cuts at: 0 385 540
Size: 385 155
Enzymes that do cut:
AarI AhdI ApaI BanI BanII BbvCI BciVI Bme1580I
BplI Bpu10I BsaI BsaXI BseRI BseYI BsgI Bsp1286I
BspMI BsrFI BssHII BstXI BtgI BtgZI DraIII EcoNI
EcoO109I Hin4I HincII MmeI MslI MspA1I PasI PspOMI
PvuII SexAI SfcI TstI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AleI AlfI AloI AlwNI ApaLI ApoI AscI
AseI AsiSI AvaI AvrII BaeI BamHI BbeI BbsI
BcgI BclI BfrBI BglI BglII BlpI BmgBI BmrI
BmtI BpmI BpuEI BsaAI BsaBI BsaHI BsaWI BsiEI
BsiHKAI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrDI
BsrGI BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoRI EcoRV FalI FseI FspI
FspAI HaeII HindIII HpaI KasI KpnI MfeI MluI
MscI NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI PacI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspXI PsrI PstI
PvuI RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI StuI StyI SwaI TaqII
TatI Tth111I XbaI XcmI XhoI XmaI ZraI