(Linear) (Six-base) MAPSORT of: KIEE1660.seq  Check: 352  from: 1  to: 531



KIEE1660.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:44  ..

AlwNI CAG_nnn'CTG

Cuts at:      0     104     116     312     531 
   Size:        104      12     196     219

  Fragments arranged by size: 

                 219     196     104      12
 
AvaI C'yCGr_G

Cuts at:      0     377     531 
   Size:        377     154
 
BanI G'GyrC_C

Cuts at:      0     448     531 
   Size:        448      83
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     408     531 
   Size:        408     123
 
BbvCI CC'TCA_GC

Cuts at:      0     154     531 
   Size:        154     377
 
BglI GCCn_nnn'nGGC

Cuts at:      0     195     531 
   Size:        195     336
 
Bme1580I G_kGCm'C

Cuts at:      0     337     451     531 
   Size:        337     114      80

  Fragments arranged by size: 

                 337     114      80
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     288     490     531 
   Size:        288     202      41

  Fragments arranged by size: 

                 288     202      41
 
Bpu10I CC'TnA_GC

Cuts at:      0     154     495     531 
   Size:        154     341      36

  Fragments arranged by size: 

                 341     154      36
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     160     531 
   Size:        160     371
 
BseYI C'CCAG_C

Cuts at:      0     109     121     531 
   Size:        109      12     410

  Fragments arranged by size: 

                 410     109      12
 
BsiHKAI G_wGCw'C

Cuts at:      0     166     531 
   Size:        166     365
 
BsmI GAATG_Cn'

Cuts at:      0      64     531 
   Size:         64     467
 
Bsp1286I G_dGCh'C

Cuts at:      0     166     337     451     531 
   Size:        166     171     114      80

  Fragments arranged by size: 

                 171     166     114      80
 
BsrDI GCAATG_nn'

Cuts at:      0      80     531 
   Size:         80     451
 
BsrFI r'CCGG_y

Cuts at:      0     296     531 
   Size:        296     235
 
BtgI C'CryG_G

Cuts at:      0     435     531 
   Size:        435      96
 
BtsI GCAGTG_nn'

Cuts at:      0     339     531 
   Size:        339     192
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     307     342     531 
   Size:        307      35     189

  Fragments arranged by size: 

                 307     189      35
 
EaeI y'GGCC_r

Cuts at:      0     427     531 
   Size:        427     104
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     288     490     531 
   Size:        288     202      41

  Fragments arranged by size: 

                 288     202      41
 
EcoO109I rG'GnC_Cy

Cuts at:      0     309     531 
   Size:        309     222
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     260     531 
   Size:        260     271
 
MspA1I CmG'CkG

Cuts at:      0     113     531 
   Size:        113     418
 
NcoI C'CATG_G

Cuts at:      0     435     531 
   Size:        435      96
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     436     531 
   Size:        436      95
 
PpuMI rG'GwC_Cy

Cuts at:      0     309     531 
   Size:        309     222
 
PvuII CAG'CTG

Cuts at:      0     113     531 
   Size:        113     418
 
SfcI C'TryA_G

Cuts at:      0      52      96     219     352     420     531 
   Size:         52      44     123     133      68     111

  Fragments arranged by size: 

                 133     123     111      68      52      44
 
SmaI CCC'GGG

Cuts at:      0     379     531 
   Size:        379     152
 
StyI C'CwwG_G

Cuts at:      0     435     531 
   Size:        435      96
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     224     466     531 
   Size:        224     242      65

  Fragments arranged by size: 

                 242     224      65
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     199     231     531 
   Size:        199      32     300

  Fragments arranged by size: 

                 300     199      32
 
XmaI C'CCGG_G

Cuts at:      0     377     531 
   Size:        377     154
 

 Enzymes that do cut:

    AlwNI     AvaI     BanI     BbsI    BbvCI     BglI Bme1580I     BpmI
   Bpu10I    BseRI    BseYI  BsiHKAI     BsmI Bsp1286I    BsrDI    BsrFI
     BtgI     BtsI    CspCI     EaeI  Eco57MI EcoO109I     MmeI   MspA1I
     NcoI    PflMI    PpuMI    PvuII     SfcI     SmaI     StyI    TaqII
     TstI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI    BanII
     BbeI     BcgI    BciVI     BclI    BfrBI    BglII     BlpI    BmgBI
     BmrI     BmtI     BplI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI     BsgI    BsiEI    BsiWI    BsmBI    BspEI    BspHI
    BspMI    BsrBI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstXI    BstYI  BstZ17I   Bsu36I    BtgZI     ClaI     DraI   DraIII
     DrdI     EagI     EarI     EciI  EcoICRI    EcoNI    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI    HaeII    Hin4I   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MscI     MslI     NaeI
     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI     PfoI     PmeI     PmlI     PpiI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     SwaI     TatI  Tth111I     XbaI     XcmI     XhoI
     XmnI     ZraI