(Linear) (Six-base) MAPSORT of: KIEE1655.seq  Check: 1283  from: 1  to: 513



KIEE1655.orf

 With 182 enzymes: SgfI * 

                        March 22, 2012 15:52  ..

AlwNI CAG_nnn'CTG

Cuts at:      0     255     513 
   Size:        255     258
 
ApoI r'AATT_y

Cuts at:      0       9     513 
   Size:          9     504
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     179     278     513 
   Size:        179      99     235

  Fragments arranged by size: 

                 235     179      99
 
BsrDI GCAATG_nn'

Cuts at:      0      56     513 
   Size:         56     457
 
Bsu36I CC'TnA_GG

Cuts at:      0     340     513 
   Size:        340     173
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     268     513 
   Size:        268     245
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     379     411     513 
   Size:        379      32     102

  Fragments arranged by size: 

                 379     102      32
 
HincII GTy'rAC

Cuts at:      0     126     286     513 
   Size:        126     160     227

  Fragments arranged by size: 

                 227     160     126
 
MfeI C'AATT_G

Cuts at:      0     292     513 
   Size:        292     221
 
PfoI T'CCnGG_A

Cuts at:      0     481     513 
   Size:        481      32
 
SspI AAT'ATT

Cuts at:      0     419     513 
   Size:        419      94
 
StyI C'CwwG_G

Cuts at:      0     100     513 
   Size:        100     413
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     349     381     513 
   Size:        349      32     132

  Fragments arranged by size: 

                 349     132      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     462     513 
   Size:        462      51
 
XmnI GAAnn'nnTTC

Cuts at:      0     251     513 
   Size:        251     262
 

 Enzymes that do cut:

    AlwNI     ApoI     BbsI    BsrDI   Bsu36I     FalI    Hin4I   HincII
     MfeI     PfoI     SspI     StyI     TstI     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I     BtgI
    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI
     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI
    EcoRV     FseI     FspI    FspAI    HaeII  HindIII     HpaI     KasI
     KpnI     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI    PflMI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SrfI     StuI     SwaI    TaqII     TatI
  Tth111I     XbaI     XhoI     XmaI     ZraI