Restriction map for
pFN21AE1648
(Linear) (Six-base) MAPSORT of: KIEE1648.seq Check: 9299 from: 1 to: 492
KIEE1648.orf
With 182 enzymes: SgfI *
January 29, 2014 11:44 ..
AlwNI CAG_nnn'CTG
Cuts at: 0 170 492
Size: 170 322
ApaI G_GGCC'C
Cuts at: 0 164 492
Size: 164 328
AvaI C'yCGr_G
Cuts at: 0 75 136 340 457 492
Size: 75 61 204 117 35
Fragments arranged by size:
204 117 75 61 35
BanI G'GyrC_C
Cuts at: 0 453 492
Size: 453 39
BanII G_rGCy'C
Cuts at: 0 164 341 464 473 492
Size: 164 177 123 9 19
Fragments arranged by size:
177 164 123 19 9
BbeI G_GCGC'C
Cuts at: 0 457 492
Size: 457 35
BbvCI CC'TCA_GC
Cuts at: 0 209 492
Size: 209 283
BglI GCCn_nnn'nGGC
Cuts at: 0 80 140 260 353 492
Size: 80 60 120 93 139
Fragments arranged by size:
139 120 93 80 60
Bme1580I G_kGCm'C
Cuts at: 0 164 205 492
Size: 164 41 287
Fragments arranged by size:
287 164 41
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 127 492
Size: 127 365
Bpu10I CC'TnA_GC
Cuts at: 0 209 492
Size: 209 283
BsaHI Gr'CG_yC
Cuts at: 0 454 485 492
Size: 454 31 7
Fragments arranged by size:
454 31 7
BsaWI w'CCGG_w
Cuts at: 0 383 492
Size: 383 109
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 107 137 492
Size: 107 30 355
Fragments arranged by size:
355 107 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 39 244 492
Size: 39 205 248
Fragments arranged by size:
248 205 39
BseYI C'CCAG_C
Cuts at: 0 163 186 476 492
Size: 163 23 290 16
Fragments arranged by size:
290 163 23 16
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 460 492
Size: 460 32
BsiEI CG_ry'CG
Cuts at: 0 384 416 492
Size: 384 32 76
Fragments arranged by size:
384 76 32
Bsp1286I G_dGCh'C
Cuts at: 0 164 205 341 464 473 492
Size: 164 41 136 123 9 19
Fragments arranged by size:
164 136 123 41 19 9
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 248 492
Size: 248 244
BsrBI CCG'CTC
Cuts at: 0 281 492
Size: 281 211
BsrFI r'CCGG_y
Cuts at: 0 411 492
Size: 411 81
BstAPI GCAn_nnn'nTGC
Cuts at: 0 173 492
Size: 173 319
BstXI CCAn_nnnn'nTGG
Cuts at: 0 402 492
Size: 402 90
EaeI y'GGCC_r
Cuts at: 0 261 413 446 492
Size: 261 152 33 46
Fragments arranged by size:
261 152 46 33
EagI C'GGCC_G
Cuts at: 0 413 492
Size: 413 79
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 127 492
Size: 127 365
EcoO109I rG'GnC_Cy
Cuts at: 0 419 492
Size: 419 73
FseI GG_CCGG'CC
Cuts at: 0 415 492
Size: 415 77
HaeII r_GCGC'y
Cuts at: 0 217 457 492
Size: 217 240 35
Fragments arranged by size:
240 217 35
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 107 139 492
Size: 107 32 353
Fragments arranged by size:
353 107 32
KasI G'GCGC_C
Cuts at: 0 453 492
Size: 453 39
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 125 492
Size: 125 367
MspA1I CmG'CkG
Cuts at: 0 152 290 402 444 492
Size: 152 138 112 42 48
Fragments arranged by size:
152 138 112 48 42
NaeI GCC'GGC
Cuts at: 0 413 492
Size: 413 79
NarI GG'CG_CC
Cuts at: 0 454 492
Size: 454 38
NgoMIV G'CCGG_C
Cuts at: 0 411 492
Size: 411 81
PspOMI G'GGCC_C
Cuts at: 0 160 492
Size: 160 332
PvuII CAG'CTG
Cuts at: 0 402 444 492
Size: 402 42 48
Fragments arranged by size:
402 48 42
SfoI GGC'GCC
Cuts at: 0 455 492
Size: 455 37
SmaI CCC'GGG
Cuts at: 0 138 492
Size: 138 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 349 492
Size: 349 143
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 102 492
Size: 102 390
XmaI C'CCGG_G
Cuts at: 0 136 492
Size: 136 356
Enzymes that do cut:
AlwNI ApaI AvaI BanI BanII BbeI BbvCI BglI
Bme1580I BpmI Bpu10I BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI Bsp1286I BspMI BsrBI BsrFI BstAPI BstXI
EaeI EagI Eco57MI EcoO109I FseI HaeII Hin4I KasI
MmeI MspA1I NaeI NarI NgoMIV PspOMI PvuII SfoI
SmaI TaqII XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI ApaLI ApoI
AscI AseI AsiSI AvrII BaeI BamHI BbsI BcgI
BciVI BclI BfrBI BglII BlpI BmgBI BmrI BmtI
BplI BpuEI BsaI BsaAI BsaBI BsiHKAI BsiWI BsmI
BsmBI BspEI BspHI BsrDI BsrGI BssHII BssSI BstBI
BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EarI EciI EcoICRI EcoNI
EcoRI EcoRV FalI FspI FspAI HincII HindIII HpaI
KpnI MfeI MluI MscI MslI NcoI NdeI NheI
NotI NruI NsiI NspI PacI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI
PsrI PstI PvuI RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI
SmlI SnaBI SpeI SphI SrfI SspI StuI StyI
SwaI TatI TstI Tth111I XbaI XhoI XmnI ZraI