(Linear) (Six-base) MAPSORT of: KIEE1647.seq  Check: 3534  from: 1  to: 489



KIEE1647.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:44  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     466     489 
   Size:        466      23
 
Acc65I G'GTAC_C

Cuts at:      0     275     489 
   Size:        275     214
 
ApoI r'AATT_y

Cuts at:      0     354     489 
   Size:        354     135
 
BanI G'GyrC_C

Cuts at:      0     275     489 
   Size:        275     214
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      16     347     489 
   Size:         16     331     142

  Fragments arranged by size: 

                 331     142      16
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     294     489 
   Size:        294     195
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     246     489 
   Size:        246     243
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     314     489 
   Size:        314     175
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     370     489 
   Size:        370     119
 
BsaAI yAC'GTr

Cuts at:      0     214     489 
   Size:        214     275
 
BsaWI w'CCGG_w

Cuts at:      0     389     489 
   Size:        389     100
 
BseYI C'CCAG_C

Cuts at:      0      24     489 
   Size:         24     465
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     438     489 
   Size:        438      51
 
BspEI T'CCGG_A

Cuts at:      0     389     489 
   Size:        389     100
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     466     489 
   Size:        466      23
 
BsrBI CCG'CTC

Cuts at:      0     230     489 
   Size:        230     259
 
BsrGI T'GTAC_A

Cuts at:      0     291     489 
   Size:        291     198
 
BtgI C'CryG_G

Cuts at:      0      29     489 
   Size:         29     460
 
DraIII CAC_nnn'GTG

Cuts at:      0     483     489 
   Size:        483       6
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     149     489 
   Size:        149     340
 
EcoO109I rG'GnC_Cy

Cuts at:      0     264     489 
   Size:        264     225
 
EcoRI G'AATT_C

Cuts at:      0     354     489 
   Size:        354     135
 
HindIII A'AGCT_T

Cuts at:      0     166     489 
   Size:        166     323
 
KpnI G_GTAC'C

Cuts at:      0     279     489 
   Size:        279     210
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     407     489 
   Size:        407      82
 
MslI CAynn'nnrTG

Cuts at:      0     349     489 
   Size:        349     140
 
NcoI C'CATG_G

Cuts at:      0      29     489 
   Size:         29     460
 
PpuMI rG'GwC_Cy

Cuts at:      0     264     489 
   Size:        264     225
 
PshAI GACnn'nnGTC

Cuts at:      0     382     489 
   Size:        382     107
 
SanDI GG'GwC_CC

Cuts at:      0     264     489 
   Size:        264     225
 
ScaI AGT'ACT

Cuts at:      0      40     489 
   Size:         40     449
 
SmlI C'TyrA_G

Cuts at:      0     329     489 
   Size:        329     160
 
StyI C'CwwG_G

Cuts at:      0      29     402     489 
   Size:         29     373      87

  Fragments arranged by size: 

                 373      87      29
 
TatI w'GTAC_w

Cuts at:      0      38     291     489 
   Size:         38     253     198

  Fragments arranged by size: 

                 253     198      38
 

 Enzymes that do cut:

     AarI   Acc65I     ApoI     BanI     BbsI     BmrI     BplI    BpuEI
     BsaI    BsaAI    BsaWI    BseYI     BsgI    BspEI    BspMI    BsrBI
    BsrGI     BtgI   DraIII    EcoNI EcoO109I    EcoRI  HindIII     KpnI
     MmeI     MslI     NcoI    PpuMI    PshAI    SanDI     ScaI     SmlI
     StyI     TatI

 Enzymes that do not cut: 

    AatII     AccI     AclI     AcuI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI    BanII     BbeI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmtI     BpmI   Bpu10I    BsaBI    BsaHI    BsaXI
    BseRI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspHI
    BsrDI    BsrFI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI
    BstYI  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI     DraI
     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoRV
     FalI     FseI     FspI    FspAI    HaeII    Hin4I   HincII     HpaI
     KasI     MfeI     MluI     MscI   MspA1I     NaeI     NarI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI    PflMI     PfoI     PmeI     PmlI     PpiI     PsiI   PspOMI
    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII
     SalI     SapI     SbfI    SexAI     SfcI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     SwaI    TaqII     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI
     XmnI     ZraI