(Linear) (Six-base) MAPSORT of: KIEE1645.seq  Check: 2646  from: 1  to: 480



KIEE1645.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:44  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      68     343     480 
   Size:         68     275     137

  Fragments arranged by size: 

                 275     137      68
 
ApoI r'AATT_y

Cuts at:      0     373     480 
   Size:        373     107
 
AseI AT'TA_AT

Cuts at:      0     137     480 
   Size:        137     343
 
BamHI G'GATC_C

Cuts at:      0     166     480 
   Size:        166     314
 
BanII G_rGCy'C

Cuts at:      0     321     424     480 
   Size:        321     103      56

  Fragments arranged by size: 

                 321     103      56
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     320     350     480 
   Size:        320      30     130

  Fragments arranged by size: 

                 320     130      30
 
BsiHKAI G_wGCw'C

Cuts at:      0     321     480 
   Size:        321     159
 
Bsp1286I G_dGCh'C

Cuts at:      0     321     424     480 
   Size:        321     103      56

  Fragments arranged by size: 

                 321     103      56
 
BstYI r'GATC_y

Cuts at:      0     166     289     480 
   Size:        166     123     191

  Fragments arranged by size: 

                 191     166     123
 
DraI TTT'AAA

Cuts at:      0     212     480 
   Size:        212     268
 
EcoICRI GAG'CTC

Cuts at:      0     319     480 
   Size:        319     161
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      68     343     480 
   Size:         68     275     137

  Fragments arranged by size: 

                 275     137      68
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     289     321     480 
   Size:        289      32     159

  Fragments arranged by size: 

                 289     159      32
 
HincII GTy'rAC

Cuts at:      0     144     246     480 
   Size:        144     102     234

  Fragments arranged by size: 

                 234     144     102
 
HpaI GTT'AAC

Cuts at:      0     144     480 
   Size:        144     336
 
SacI G_AGCT'C

Cuts at:      0     321     480 
   Size:        321     159
 
StyI C'CwwG_G

Cuts at:      0     199     480 
   Size:        199     281
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     320     352     480 
   Size:        320      32     128

  Fragments arranged by size: 

                 320     128      32
 

 Enzymes that do cut:

     AcuI     ApoI     AseI    BamHI    BanII    BsaXI  BsiHKAI Bsp1286I
    BstYI     DraI  EcoICRI  Eco57MI    Hin4I   HincII     HpaI     SacI
     StyI     TstI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI    AsiSI     AvaI    AvrII     BaeI     BanI     BbeI     BbsI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BseRI    BseYI     BsgI
    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI
   DraIII     DrdI     EaeI     EagI     EarI     EciI    EcoNI EcoO109I
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII  HindIII
     KasI     KpnI     MfeI     MluI     MmeI     MscI     MslI   MspA1I
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PstI     PvuI    PvuII    RsrII    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     SwaI    TaqII     TatI  Tth111I     XbaI     XcmI     XhoI     XmaI
     XmnI     ZraI