(Linear) (Six-base) MAPSORT of: KIEE1644.seq  Check: 6358  from: 1  to: 477



KIEE1644.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:44  ..

BanI G'GyrC_C

Cuts at:      0     326     477 
   Size:        326     151
 
Bme1580I G_kGCm'C

Cuts at:      0     331     477 
   Size:        331     146
 
Bpu10I CC'TnA_GC

Cuts at:      0     315     477 
   Size:        315     162
 
BsaHI Gr'CG_yC

Cuts at:      0     444     477 
   Size:        444      33
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     393     477 
   Size:        393      84
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     204     477 
   Size:        204     273
 
Bsp1286I G_dGCh'C

Cuts at:      0     331     477 
   Size:        331     146
 
BsrDI GCAATG_nn'

Cuts at:      0     219     374     477 
   Size:        219     155     103

  Fragments arranged by size: 

                 219     155     103
 
BsrFI r'CCGG_y

Cuts at:      0     262     477 
   Size:        262     215
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     421     456     477 
   Size:        421      35      21

  Fragments arranged by size: 

                 421      35      21
 
EaeI y'GGCC_r

Cuts at:      0     214     477 
   Size:        214     263
 
EarI CTCTTCn'nnn_

Cuts at:      0     370     477 
   Size:        370     107
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     330     477 
   Size:        330     147
 
HincII GTy'rAC

Cuts at:      0      80     477 
   Size:         80     397
 
HpaI GTT'AAC

Cuts at:      0      80     477 
   Size:         80     397
 
NaeI GCC'GGC

Cuts at:      0     264     477 
   Size:        264     213
 
NgoMIV G'CCGG_C

Cuts at:      0     262     477 
   Size:        262     215
 
ScaI AGT'ACT

Cuts at:      0     135     477 
   Size:        135     342
 
TatI w'GTAC_w

Cuts at:      0     133     477 
   Size:        133     344
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     408     477 
   Size:        408      69
 
XmnI GAAnn'nnTTC

Cuts at:      0      49     477 
   Size:         49     428
 

 Enzymes that do cut:

     BanI Bme1580I   Bpu10I    BsaHI    BseRI    BsmBI Bsp1286I    BsrDI
    BsrFI    CspCI     EaeI     EarI     FalI   HincII     HpaI     NaeI
   NgoMIV     ScaI     TatI     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI
    BamHI    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI
    BfrBI     BglI    BglII     BlpI    BmgBI     BmrI     BmtI     BplI
     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaWI    BsaXI    BseYI
     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BspEI    BspHI    BspMI
    BsrBI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI
    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI     DraI
   DraIII     DrdI     EagI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I
    EcoRI    EcoRV     FseI     FspI    FspAI    HaeII    Hin4I  HindIII
     KasI     KpnI     MfeI     MluI     MmeI     MscI     MslI   MspA1I
     NarI     NcoI     NdeI     NheI     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI     SwaI
    TaqII     TstI  Tth111I     XbaI     XhoI     XmaI     ZraI