Restriction map for
pFN21AE1642
(Linear) (Six-base) MAPSORT of: KIEE1642.seq Check: 2391 from: 1 to: 468
KIEE1642.orf
With 182 enzymes: SgfI *
July 15, 2010 18:44 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 323 468
Size: 323 145
AleI CACnn'nnGTG
Cuts at: 0 370 468
Size: 370 98
AlwNI CAG_nnn'CTG
Cuts at: 0 294 468
Size: 294 174
AvaI C'yCGr_G
Cuts at: 0 72 203 385 468
Size: 72 131 182 83
Fragments arranged by size:
182 131 83 72
BanI G'GyrC_C
Cuts at: 0 149 170 215 318 360 468
Size: 149 21 45 103 42 108
Fragments arranged by size:
149 108 103 45 42 21
BanII G_rGCy'C
Cuts at: 0 91 131 392 413 468
Size: 91 40 261 21 55
Fragments arranged by size:
261 91 55 40 21
BbeI G_GCGC'C
Cuts at: 0 153 219 322 364 468
Size: 153 66 103 42 104
Fragments arranged by size:
153 104 103 66 42
BbsI GAAGACnn'nnnn_
Cuts at: 0 273 468
Size: 273 195
BbvCI CC'TCA_GC
Cuts at: 0 433 468
Size: 433 35
BglII A'GATC_T
Cuts at: 0 101 468
Size: 101 367
Bme1580I G_kGCm'C
Cuts at: 0 173 468
Size: 173 295
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 314 430 468
Size: 314 116 38
Fragments arranged by size:
314 116 38
Bpu10I CC'TnA_GC
Cuts at: 0 433 468
Size: 433 35
BsaHI Gr'CG_yC
Cuts at: 0 150 216 319 338 361 468
Size: 150 66 103 19 23 107
Fragments arranged by size:
150 107 103 66 23 19
BsaWI w'CCGG_w
Cuts at: 0 91 468
Size: 91 377
BseYI C'CCAG_C
Cuts at: 0 154 243 468
Size: 154 89 225
Fragments arranged by size:
225 154 89
BsiEI CG_ry'CG
Cuts at: 0 461 468
Size: 461 7
Bsp1286I G_dGCh'C
Cuts at: 0 91 131 173 392 413 468
Size: 91 40 42 219 21 55
Fragments arranged by size:
219 91 55 42 40 21
BsrFI r'CCGG_y
Cuts at: 0 315 468
Size: 315 153
BstYI r'GATC_y
Cuts at: 0 101 290 468
Size: 101 189 178
Fragments arranged by size:
189 178 101
EaeI y'GGCC_r
Cuts at: 0 458 468
Size: 458 10
EagI C'GGCC_G
Cuts at: 0 458 468
Size: 458 10
EarI CTCTTCn'nnn_
Cuts at: 0 299 468
Size: 299 169
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 314 323 430 468
Size: 314 9 107 38
Fragments arranged by size:
314 107 38 9
EcoO109I rG'GnC_Cy
Cuts at: 0 45 76 468
Size: 45 31 392
Fragments arranged by size:
392 45 31
HaeII r_GCGC'y
Cuts at: 0 153 161 219 322 364 468
Size: 153 8 58 103 42 104
Fragments arranged by size:
153 104 103 58 42 8
KasI G'GCGC_C
Cuts at: 0 149 215 318 360 468
Size: 149 66 103 42 108
Fragments arranged by size:
149 108 103 66 42
MslI CAynn'nnrTG
Cuts at: 0 370 468
Size: 370 98
MspA1I CmG'CkG
Cuts at: 0 17 136 222 468
Size: 17 119 86 246
Fragments arranged by size:
246 119 86 17
NaeI GCC'GGC
Cuts at: 0 317 468
Size: 317 151
NarI GG'CG_CC
Cuts at: 0 150 216 319 361 468
Size: 150 66 103 42 107
Fragments arranged by size:
150 107 103 66 42
NgoMIV G'CCGG_C
Cuts at: 0 315 468
Size: 315 153
PfoI T'CCnGG_A
Cuts at: 0 122 468
Size: 122 346
PpuMI rG'GwC_Cy
Cuts at: 0 76 468
Size: 76 392
PspXI vC'TCGA_Gb
Cuts at: 0 203 385 468
Size: 203 182 83
Fragments arranged by size:
203 182 83
PstI C_TGCA'G
Cuts at: 0 44 468
Size: 44 424
PvuII CAG'CTG
Cuts at: 0 17 222 468
Size: 17 205 246
Fragments arranged by size:
246 205 17
SanDI GG'GwC_CC
Cuts at: 0 76 468
Size: 76 392
SbfI CC_TGCA'GG
Cuts at: 0 44 468
Size: 44 424
SfcI C'TryA_G
Cuts at: 0 40 468
Size: 40 428
SfoI GGC'GCC
Cuts at: 0 151 217 320 362 468
Size: 151 66 103 42 106
Fragments arranged by size:
151 106 103 66 42
SmaI CCC'GGG
Cuts at: 0 74 468
Size: 74 394
SmlI C'TyrA_G
Cuts at: 0 203 385 468
Size: 203 182 83
Fragments arranged by size:
203 182 83
XhoI C'TCGA_G
Cuts at: 0 203 385 468
Size: 203 182 83
Fragments arranged by size:
203 182 83
XmaI C'CCGG_G
Cuts at: 0 72 468
Size: 72 396
Enzymes that do cut:
AcuI AleI AlwNI AvaI BanI BanII BbeI BbsI
BbvCI BglII Bme1580I BpmI Bpu10I BsaHI BsaWI BseYI
BsiEI Bsp1286I BsrFI BstYI EaeI EagI EarI Eco57MI
EcoO109I HaeII KasI MslI MspA1I NaeI NarI NgoMIV
PfoI PpuMI PspXI PstI PvuII SanDI SbfI SfcI
SfoI SmaI SmlI XhoI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AlfI AloI ApaI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BcgI BciVI BclI
BfrBI BglI BlpI BmgBI BmrI BmtI BplI BpuEI
BsaI BsaAI BsaBI BsaXI BseRI BsgI BsiHKAI BsiWI
BsmI BsmBI BspEI BspHI BspMI BsrBI BsrDI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI
FspAI Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI NcoI NdeI NheI NotI NruI NsiI
NspI PacI PasI PciI PflMI PmeI PmlI PpiI
PshAI PsiI PspOMI PsrI PvuI RsrII SacI SacII
SalI SapI ScaI SexAI SfiI SgfI SgrAI SnaBI
SpeI SphI SrfI SspI StuI StyI SwaI TaqII
TatI TstI Tth111I XbaI XcmI XmnI ZraI