(Linear) (Six-base) MAPSORT of: KIEE1641.seq  Check: 9234  from: 1  to: 465



KIEE1641.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:44  ..

ApaI G_GGCC'C

Cuts at:      0     341     465 
   Size:        341     124
 
AvaI C'yCGr_G

Cuts at:      0     359     465 
   Size:        359     106
 
BanII G_rGCy'C

Cuts at:      0     341     465 
   Size:        341     124
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     451     465 
   Size:        451      14
 
BglI GCCn_nnn'nGGC

Cuts at:      0     233     465 
   Size:        233     232
 
Bme1580I G_kGCm'C

Cuts at:      0     341     465 
   Size:        341     124
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     148     336     465 
   Size:        148     188     129

  Fragments arranged by size: 

                 188     148     129
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      42     118     238     465 
   Size:         42      76     120     227

  Fragments arranged by size: 

                 227     120      76      42
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      55     465 
   Size:         55     410
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     444     465 
   Size:        444      21
 
BseYI C'CCAG_C

Cuts at:      0     395     465 
   Size:        395      70
 
BsiEI CG_ry'CG

Cuts at:      0     237     465 
   Size:        237     228
 
BsmI GAATG_Cn'

Cuts at:      0     425     465 
   Size:        425      40
 
Bsp1286I G_dGCh'C

Cuts at:      0     341     465 
   Size:        341     124
 
BsrFI r'CCGG_y

Cuts at:      0     232     465 
   Size:        232     233
 
BssHII G'CGCG_C

Cuts at:      0     406     465 
   Size:        406      59
 
BtgI C'CryG_G

Cuts at:      0     194     465 
   Size:        194     271
 
EaeI y'GGCC_r

Cuts at:      0     197     234     368     465 
   Size:        197      37     134      97

  Fragments arranged by size: 

                 197     134      97      37
 
EagI C'GGCC_G

Cuts at:      0     234     465 
   Size:        234     231
 
EarI CTCTTCn'nnn_

Cuts at:      0      53     100     465 
   Size:         53      47     365

  Fragments arranged by size: 

                 365      53      47
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      42     118     238     465 
   Size:         42      76     120     227

  Fragments arranged by size: 

                 227     120      76      42
 
EcoO109I rG'GnC_Cy

Cuts at:      0     338     465 
   Size:        338     127
 
MscI TGG'CCA

Cuts at:      0     370     465 
   Size:        370      95
 
MspA1I CmG'CkG

Cuts at:      0      20     205     465 
   Size:         20     185     260

  Fragments arranged by size: 

                 260     185      20
 
NdeI CA'TA_TG

Cuts at:      0     251     465 
   Size:        251     214
 
PshAI GACnn'nnGTC

Cuts at:      0      67     465 
   Size:         67     398
 
PspOMI G'GGCC_C

Cuts at:      0     337     465 
   Size:        337     128
 
PstI C_TGCA'G

Cuts at:      0     430     465 
   Size:        430      35
 
PvuII CAG'CTG

Cuts at:      0      20     465 
   Size:         20     445
 
SapI GCTCTTCn'nnn_

Cuts at:      0     100     465 
   Size:        100     365
 
SbfI CC_TGCA'GG

Cuts at:      0     430     465 
   Size:        430      35
 
SfcI C'TryA_G

Cuts at:      0     426     465 
   Size:        426      39
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     233     465 
   Size:        233     232
 
SmaI CCC'GGG

Cuts at:      0     361     465 
   Size:        361     104
 
XmaI C'CCGG_G

Cuts at:      0     359     465 
   Size:        359     106
 
XmnI GAAnn'nnTTC

Cuts at:      0     436     448     465 
   Size:        436      12      17

  Fragments arranged by size: 

                 436      17      12
 

 Enzymes that do cut:

     ApaI     AvaI    BanII     BbsI     BglI Bme1580I     BplI     BpmI
     BsaI    BseRI    BseYI    BsiEI     BsmI Bsp1286I    BsrFI   BssHII
     BtgI     EaeI     EagI     EarI  Eco57MI EcoO109I     MscI   MspA1I
     NdeI    PshAI   PspOMI     PstI    PvuII     SapI     SbfI     SfcI
     SfiI     SmaI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI    ApaLI
     ApoI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BanI
     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI    BglII     BlpI
    BmgBI     BmrI     BmtI   Bpu10I    BpuEI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI     BsgI  BsiHKAI    BsiWI    BsmBI    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrGI    BssSI   BstAPI    BstBI   BstEII
    BstXI    BstYI  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI
     DraI   DraIII     DrdI     EciI  EcoICRI    EcoNI    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI    HaeII    Hin4I   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MslI     NaeI
     NarI     NcoI   NgoMIV     NheI     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI
    PpuMI     PsiI    PspXI     PsrI     PvuI    RsrII     SacI    SacII
     SalI    SanDI     ScaI    SexAI     SfoI     SgfI    SgrAI     SmlI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI     SwaI
    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     ZraI