Restriction map for
pFN21AE1637
(Linear) (Six-base) MAPSORT of: KIEE1637.seq Check: 8051 from: 1 to: 456
KIEE1637.orf
With 182 enzymes: SgfI *
July 15, 2010 18:43 ..
AccI GT'mk_AC
Cuts at: 0 263 456
Size: 263 193
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 79 456
Size: 79 377
ApaI G_GGCC'C
Cuts at: 0 213 456
Size: 213 243
AvaI C'yCGr_G
Cuts at: 0 139 456
Size: 139 317
BanI G'GyrC_C
Cuts at: 0 366 456
Size: 366 90
BanII G_rGCy'C
Cuts at: 0 213 456
Size: 213 243
BbsI GAAGACnn'nnnn_
Cuts at: 0 306 456
Size: 306 150
BglI GCCn_nnn'nGGC
Cuts at: 0 40 418 456
Size: 40 378 38
Fragments arranged by size:
378 40 38
Bme1580I G_kGCm'C
Cuts at: 0 213 369 456
Size: 213 156 87
Fragments arranged by size:
213 156 87
BmgBI CAC'GTC
Cuts at: 0 192 456
Size: 192 264
BmrI ACTGGGnnnn_n'
Cuts at: 0 162 456
Size: 162 294
BsaI GGTCTCn'nnnn_
Cuts at: 0 223 456
Size: 223 233
BsaAI yAC'GTr
Cuts at: 0 84 456
Size: 84 372
BsaWI w'CCGG_w
Cuts at: 0 215 456
Size: 215 241
BseYI C'CCAG_C
Cuts at: 0 398 456
Size: 398 58
Bsp1286I G_dGCh'C
Cuts at: 0 213 369 456
Size: 213 156 87
Fragments arranged by size:
213 156 87
BsrBI CCG'CTC
Cuts at: 0 377 456
Size: 377 79
BssSI C'ACGA_G
Cuts at: 0 319 456
Size: 319 137
BstYI r'GATC_y
Cuts at: 0 428 456
Size: 428 28
Bsu36I CC'TnA_GG
Cuts at: 0 205 456
Size: 205 251
BtgI C'CryG_G
Cuts at: 0 341 416 456
Size: 341 75 40
Fragments arranged by size:
341 75 40
DraIII CAC_nnn'GTG
Cuts at: 0 342 456
Size: 342 114
EaeI y'GGCC_r
Cuts at: 0 89 456
Size: 89 367
EcoO109I rG'GnC_Cy
Cuts at: 0 163 209 277 456
Size: 163 46 68 179
Fragments arranged by size:
179 163 68 46
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 144 456
Size: 144 312
MscI TGG'CCA
Cuts at: 0 91 456
Size: 91 365
MslI CAynn'nnrTG
Cuts at: 0 76 456
Size: 76 380
NcoI C'CATG_G
Cuts at: 0 416 456
Size: 416 40
PflMI CCAn_nnn'nTGG
Cuts at: 0 15 456
Size: 15 441
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 188 220 456
Size: 188 32 236
Fragments arranged by size:
236 188 32
PpuMI rG'GwC_Cy
Cuts at: 0 277 456
Size: 277 179
PspOMI G'GGCC_C
Cuts at: 0 209 456
Size: 209 247
PstI C_TGCA'G
Cuts at: 0 58 428 456
Size: 58 370 28
Fragments arranged by size:
370 58 28
SfcI C'TryA_G
Cuts at: 0 54 424 456
Size: 54 370 32
Fragments arranged by size:
370 54 32
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 40 456
Size: 40 416
StuI AGG'CCT
Cuts at: 0 127 456
Size: 127 329
StyI C'CwwG_G
Cuts at: 0 416 456
Size: 416 40
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 384 456
Size: 384 72
Enzymes that do cut:
AccI AlfI ApaI AvaI BanI BanII BbsI BglI
Bme1580I BmgBI BmrI BsaI BsaAI BsaWI BseYI Bsp1286I
BsrBI BssSI BstYI Bsu36I BtgI DraIII EaeI EcoO109I
FalI MscI MslI NcoI PflMI PpiI PpuMI PspOMI
PstI SfcI SfiI StuI StyI TaqII
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AloI AlwNI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BbeI BbvCI BcgI
BciVI BclI BfrBI BglII BlpI BmtI BplI BpmI
Bpu10I BpuEI BsaBI BsaHI BsaXI BseRI BsgI BsiEI
BsiHKAI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrDI
BsrFI BsrGI BssHII BstAPI BstBI BstEII BstXI BstZ17I
BtgZI BtsI ClaI CspCI DraI DrdI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoRI EcoRV FseI FspI
FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MspA1I NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspI PacI PasI PciI
PfoI PmeI PmlI PshAI PsiI PspXI PsrI PvuI
PvuII RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI SwaI TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI