(Linear) (Six-base) MAPSORT of: KIEE1636.seq  Check: 9840  from: 1  to: 456



KIEE1636.orf

 With 182 enzymes: SgfI * 

                        December 27, 2011 16:31  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     312     456 
   Size:        312     144
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     123     456 
   Size:        123     333
 
AlwNI CAG_nnn'CTG

Cuts at:      0      81     456 
   Size:         81     375
 
BamHI G'GATC_C

Cuts at:      0     179     456 
   Size:        179     277
 
BanII G_rGCy'C

Cuts at:      0     383     419     456 
   Size:        383      36      37

  Fragments arranged by size: 

                 383      37      36
 
BbvCI CC'TCA_GC

Cuts at:      0     420     456 
   Size:        420      36
 
BglII A'GATC_T

Cuts at:      0     122     456 
   Size:        122     334
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     262     456 
   Size:        262     194
 
Bpu10I CC'TnA_GC

Cuts at:      0     315     420     456 
   Size:        315     105      36

  Fragments arranged by size: 

                 315     105      36
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     355     456 
   Size:        355     101
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      80     110     456 
   Size:         80      30     346

  Fragments arranged by size: 

                 346      80      30
 
BseYI C'CCAG_C

Cuts at:      0     383     456 
   Size:        383      73
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     111     134     456 
   Size:        111      23     322

  Fragments arranged by size: 

                 322     111      23
 
Bsp1286I G_dGCh'C

Cuts at:      0     383     419     456 
   Size:        383      36      37

  Fragments arranged by size: 

                 383      37      36
 
BsrGI T'GTAC_A

Cuts at:      0     266     456 
   Size:        266     190
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     174     456 
   Size:        174     282
 
BstYI r'GATC_y

Cuts at:      0      90     122     179     456 
   Size:         90      32      57     277

  Fragments arranged by size: 

                 277      90      57      32
 
BtgI C'CryG_G

Cuts at:      0      14     456 
   Size:         14     442
 
EaeI y'GGCC_r

Cuts at:      0      72     456 
   Size:         72     384
 
EarI CTCTTCn'nnn_

Cuts at:      0     215     275     456 
   Size:        215      60     181

  Fragments arranged by size: 

                 215     181      60
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     312     456 
   Size:        312     144
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     262     312     456 
   Size:        262      50     144

  Fragments arranged by size: 

                 262     144      50
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     140     456 
   Size:        140     316
 
EcoO109I rG'GnC_Cy

Cuts at:      0     162     226     456 
   Size:        162      64     230

  Fragments arranged by size: 

                 230     162      64
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     456 
   Size:        456
 
MscI TGG'CCA

Cuts at:      0      74     456 
   Size:         74     382
 
MspA1I CmG'CkG

Cuts at:      0      78     197     240     456 
   Size:         78     119      43     216

  Fragments arranged by size: 

                 216     119      78      43
 
NcoI C'CATG_G

Cuts at:      0      14     456 
   Size:         14     442
 
NspI r_CATG'y

Cuts at:      0     209     456 
   Size:        209     247
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      81     456 
   Size:         81     375
 
PpuMI rG'GwC_Cy

Cuts at:      0     162     456 
   Size:        162     294
 
PvuII CAG'CTG

Cuts at:      0      78     240     456 
   Size:         78     162     216

  Fragments arranged by size: 

                 216     162      78
 
SfcI C'TryA_G

Cuts at:      0     235     456 
   Size:        235     221
 
SmlI C'TyrA_G

Cuts at:      0     334     456 
   Size:        334     122
 
StyI C'CwwG_G

Cuts at:      0      14     158     404     456 
   Size:         14     144     246      52

  Fragments arranged by size: 

                 246     144      52      14
 
TatI w'GTAC_w

Cuts at:      0     266     393     456 
   Size:        266     127      63

  Fragments arranged by size: 

                 266     127      63
 

 Enzymes that do cut:

     AcuI     AlfI    AlwNI    BamHI    BanII    BbvCI    BglII     BpmI
   Bpu10I    BpuEI    BsaXI    BseYI     BsgI Bsp1286I    BsrGI    BstXI
    BstYI     BtgI     EaeI     EarI     EciI  Eco57MI    EcoNI EcoO109I
     FalI     MscI   MspA1I     NcoI     NspI    PflMI    PpuMI    PvuII
     SfcI     SmlI     StyI     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AloI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI     BanI     BbeI     BbsI
     BcgI    BciVI     BclI    BfrBI     BglI     BlpI Bme1580I    BmgBI
     BmrI     BmtI     BplI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BseRI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI   BssHII    BssSI   BstAPI    BstBI
   BstEII  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI     DraI
   DraIII     DrdI     EagI  EcoICRI    EcoRI    EcoRV     FseI     FspI
    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     MslI     NaeI     NarI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     PacI     PasI     PciI     PfoI
     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PstI     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI    TaqII
     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI