(Linear) (Six-base) MAPSORT of: KIEE1627.seq  Check: 9203  from: 1  to: 426



KIEE1627.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:43  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0      82     198     426 
   Size:         82     116     228

  Fragments arranged by size: 

                 228     116      82
 
ApoI r'AATT_y

Cuts at:      0     392     426 
   Size:        392      34
 
AseI AT'TA_AT

Cuts at:      0     176     426 
   Size:        176     250
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     263     297     426 
   Size:        263      34     129

  Fragments arranged by size: 

                 263     129      34
 
Bme1580I G_kGCm'C

Cuts at:      0     141     426 
   Size:        141     285
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     372     426 
   Size:        372      54
 
BseYI C'CCAG_C

Cuts at:      0     293     426 
   Size:        293     133
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     151     426 
   Size:        151     275
 
BsiEI CG_ry'CG

Cuts at:      0     273     426 
   Size:        273     153
 
Bsp1286I G_dGCh'C

Cuts at:      0     141     426 
   Size:        141     285
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      82     198     426 
   Size:         82     116     228

  Fragments arranged by size: 

                 228     116      82
 
BtgI C'CryG_G

Cuts at:      0      53     426 
   Size:         53     373
 
BtsI GCAGTG_nn'

Cuts at:      0      22     426 
   Size:         22     404
 
EaeI y'GGCC_r

Cuts at:      0      50      56     426 
   Size:         50       6     370

  Fragments arranged by size: 

                 370      50       6
 
EarI CTCTTCn'nnn_

Cuts at:      0     341     426 
   Size:        341      85
 
EcoO109I rG'GnC_Cy

Cuts at:      0      83     426 
   Size:         83     343
 
NspI r_CATG'y

Cuts at:      0     284     426 
   Size:        284     142
 
PasI CC'CwG_GG

Cuts at:      0     380     426 
   Size:        380      46
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     334     366     426 
   Size:        334      32      60

  Fragments arranged by size: 

                 334      60      32
 
PpuMI rG'GwC_Cy

Cuts at:      0      83     426 
   Size:         83     343
 
PshAI GACnn'nnGTC

Cuts at:      0     311     426 
   Size:        311     115
 
PstI C_TGCA'G

Cuts at:      0     209     426 
   Size:        209     217
 
ScaI AGT'ACT

Cuts at:      0     190     426 
   Size:        190     236
 
SfcI C'TryA_G

Cuts at:      0     192     205     426 
   Size:        192      13     221

  Fragments arranged by size: 

                 221     192      13
 
StyI C'CwwG_G

Cuts at:      0      86     426 
   Size:         86     340
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     226     259     426 
   Size:        226      33     167

  Fragments arranged by size: 

                 226     167      33
 
TatI w'GTAC_w

Cuts at:      0     161     188     426 
   Size:        161      27     238

  Fragments arranged by size: 

                 238     161      27
 

 Enzymes that do cut:

     AarI     ApoI     AseI     BcgI Bme1580I     BplI    BseYI     BsgI
    BsiEI Bsp1286I    BspMI     BtgI     BtsI     EaeI     EarI EcoO109I
     NspI     PasI     PpiI    PpuMI    PshAI     PstI     ScaI     SfcI
     StyI    TaqII     TatI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI     BbsI    BbvCI    BciVI     BclI    BfrBI     BglI    BglII
     BlpI    BmgBI     BmrI     BmtI     BpmI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI  BsiHKAI    BsiWI
     BsmI    BsmBI    BspEI    BspHI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I
   Bsu36I    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI     EagI
     EciI  EcoICRI  Eco57MI    EcoNI    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MmeI     MscI     MslI   MspA1I     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     PacI     PciI    PflMI     PfoI     PmeI     PmlI     PsiI   PspOMI
    PspXI     PsrI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     SwaI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI
     ZraI