Restriction map for
pFN21AE1624
(Linear) (Six-base) MAPSORT of: KIEE1624.seq Check: 2629 from: 1 to: 411
KIEE1624.orf
With 182 enzymes: SgfI *
March 22, 2012 15:51 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 204 246 252 411
Size: 204 42 6 159
Fragments arranged by size:
204 159 42 6
AhdI GACnn_n'nnGTC
Cuts at: 0 126 411
Size: 126 285
ApoI r'AATT_y
Cuts at: 0 285 411
Size: 285 126
BglI GCCn_nnn'nGGC
Cuts at: 0 40 411
Size: 40 371
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 190 411
Size: 190 221
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 67 97 315 345 411
Size: 67 30 218 30 66
Fragments arranged by size:
218 67 66 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 175 323 411
Size: 175 148 88
Fragments arranged by size:
175 148 88
BseYI C'CCAG_C
Cuts at: 0 306 411
Size: 306 105
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 257 411
Size: 257 154
BsiEI CG_ry'CG
Cuts at: 0 35 44 71 411
Size: 35 9 27 340
Fragments arranged by size:
340 35 27 9
BsiHKAI G_wGCw'C
Cuts at: 0 167 411
Size: 167 244
Bsp1286I G_dGCh'C
Cuts at: 0 167 411
Size: 167 244
BsrDI GCAATG_nn'
Cuts at: 0 377 411
Size: 377 34
BssSI C'ACGA_G
Cuts at: 0 139 411
Size: 139 272
BtgI C'CryG_G
Cuts at: 0 11 29 38 71 411
Size: 11 18 9 33 340
Fragments arranged by size:
340 33 18 11 9
EaeI y'GGCC_r
Cuts at: 0 32 41 68 116 411
Size: 32 9 27 48 295
Fragments arranged by size:
295 48 32 27 9
EagI C'GGCC_G
Cuts at: 0 32 41 68 411
Size: 32 9 27 343
Fragments arranged by size:
343 32 27 9
EarI CTCTTCn'nnn_
Cuts at: 0 317 411
Size: 317 94
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 190 204 246 252 411
Size: 190 14 42 6 159
Fragments arranged by size:
190 159 42 14 6
EcoO109I rG'GnC_Cy
Cuts at: 0 78 411
Size: 78 333
EcoRI G'AATT_C
Cuts at: 0 285 411
Size: 285 126
HaeII r_GCGC'y
Cuts at: 0 147 197 411
Size: 147 50 214
Fragments arranged by size:
214 147 50
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 67 99 112 144 411
Size: 67 32 13 32 267
Fragments arranged by size:
267 67 32 32 13
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 65 316 411
Size: 65 251 95
Fragments arranged by size:
251 95 65
MspA1I CmG'CkG
Cuts at: 0 13 31 40 73 215 411
Size: 13 18 9 33 142 196
Fragments arranged by size:
196 142 33 18 13 9
NotI GC'GGCC_GC
Cuts at: 0 32 41 68 411
Size: 32 9 27 343
Fragments arranged by size:
343 32 27 9
PpuMI rG'GwC_Cy
Cuts at: 0 78 411
Size: 78 333
SacII CC_GC'GG
Cuts at: 0 14 32 41 74 411
Size: 14 18 9 33 337
Fragments arranged by size:
337 33 18 14 9
SapI GCTCTTCn'nnn_
Cuts at: 0 317 411
Size: 317 94
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 40 411
Size: 40 371
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 79 111 315 347 411
Size: 79 32 204 32 64
Fragments arranged by size:
204 79 64 32 32
XmnI GAAnn'nnTTC
Cuts at: 0 259 411
Size: 259 152
Enzymes that do cut:
AcuI AhdI ApoI BglI BpmI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BsrDI BssSI BtgI EaeI
EagI EarI Eco57MI EcoO109I EcoRI HaeII Hin4I MmeI
MspA1I NotI PpuMI SacII SapI SfiI TstI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AleI AlfI AloI AlwNI ApaI ApaLI AscI
AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsiWI BsmI BsmBI
BspEI BspHI BspMI BsrBI BsrFI BsrGI BssHII BstAPI
BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EciI EcoICRI EcoNI
EcoRV FalI FseI FspI FspAI HincII HindIII HpaI
KasI KpnI MfeI MluI MscI MslI NaeI NarI
NcoI NdeI NgoMIV NheI NruI NsiI NspI PacI
PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI
PsiI PspOMI PspXI PsrI PstI PvuI PvuII RsrII
SacI SalI SanDI SbfI ScaI SexAI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI StyI SwaI TaqII TatI Tth111I XbaI
XcmI XhoI XmaI ZraI