(Linear) (Six-base) MAPSORT of: KIEE1623.seq  Check: 3797  from: 1  to: 411



KIEE1623.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:43  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     397     411 
   Size:        397      14
 
AvaI C'yCGr_G

Cuts at:      0      62     411 
   Size:         62     349
 
BamHI G'GATC_C

Cuts at:      0      52     411 
   Size:         52     359
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     335     411 
   Size:        335      76
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     162     274     411 
   Size:        162     112     137

  Fragments arranged by size: 

                 162     137     112
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      12     411 
   Size:         12     399
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     159     411 
   Size:        159     252
 
BsiHKAI G_wGCw'C

Cuts at:      0     286     411 
   Size:        286     125
 
BsmI GAATG_Cn'

Cuts at:      0     377     411 
   Size:        377      34
 
Bsp1286I G_dGCh'C

Cuts at:      0     286     411 
   Size:        286     125
 
BspHI T'CATG_A

Cuts at:      0      92     411 
   Size:         92     319
 
BstYI r'GATC_y

Cuts at:      0      52     411 
   Size:         52     359
 
BtgI C'CryG_G

Cuts at:      0      11     411 
   Size:         11     400
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     162     274     397     411 
   Size:        162     112     123      14

  Fragments arranged by size: 

                 162     123     112      14
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     144     159     176     191     411 
   Size:        144      15      17      15     220

  Fragments arranged by size: 

                 220     144      17      15      15
 
HindIII A'AGCT_T

Cuts at:      0     103     411 
   Size:        103     308
 
NspI r_CATG'y

Cuts at:      0     138     411 
   Size:        138     273
 
PfoI T'CCnGG_A

Cuts at:      0     251     411 
   Size:        251     160
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      73     105     411 
   Size:         73      32     306

  Fragments arranged by size: 

                 306      73      32
 
PstI C_TGCA'G

Cuts at:      0     186     411 
   Size:        186     225
 
ScaI AGT'ACT

Cuts at:      0     125     411 
   Size:        125     286
 
SfcI C'TryA_G

Cuts at:      0     182     411 
   Size:        182     229
 
SmaI CCC'GGG

Cuts at:      0      64     411 
   Size:         64     347
 
SpeI A'CTAG_T

Cuts at:      0     199     411 
   Size:        199     212
 
StyI C'CwwG_G

Cuts at:      0      48     411 
   Size:         48     363
 
TatI w'GTAC_w

Cuts at:      0     123     411 
   Size:        123     288
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     221     253     411 
   Size:        221      32     158

  Fragments arranged by size: 

                 221     158      32
 
XmaI C'CCGG_G

Cuts at:      0      62     411 
   Size:         62     349
 

 Enzymes that do cut:

     AcuI     AvaI    BamHI     BmrI     BpmI     BsaI    BseRI  BsiHKAI
     BsmI Bsp1286I    BspHI    BstYI     BtgI  Eco57MI    Hin4I  HindIII
     NspI     PfoI     PpiI     PstI     ScaI     SfcI     SmaI     SpeI
     StyI     TatI     TstI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI    AvrII     BaeI     BanI    BanII
     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmtI     BplI   Bpu10I    BpuEI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseYI     BsgI    BsiEI
    BsiWI    BsmBI    BspEI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I
    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI
     EagI     EarI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI    HaeII   HincII     HpaI     KasI
     KpnI     MfeI     MluI     MmeI     MscI     MslI   MspA1I     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     PacI     PasI     PciI    PflMI     PmeI     PmlI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmlI    SnaBI     SphI     SrfI     SspI     StuI
     SwaI    TaqII  Tth111I     XbaI     XcmI     XhoI     XmnI     ZraI