(Linear) (Six-base) MAPSORT of: KIEE1622.seq  Check: 5347  from: 1  to: 408



KIEE1622.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:43  ..

AflIII A'CryG_T

Cuts at:      0     270     408 
   Size:        270     138
 
AhdI GACnn_n'nnGTC

Cuts at:      0     274     408 
   Size:        274     134
 
ApaI G_GGCC'C

Cuts at:      0      56     408 
   Size:         56     352
 
ApoI r'AATT_y

Cuts at:      0     380     408 
   Size:        380      28
 
BanII G_rGCy'C

Cuts at:      0      56     161     408 
   Size:         56     105     247

  Fragments arranged by size: 

                 247     105      56
 
Bme1580I G_kGCm'C

Cuts at:      0      56     408 
   Size:         56     352
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     109     408 
   Size:        109     299
 
Bpu10I CC'TnA_GC

Cuts at:      0     305     408 
   Size:        305     103
 
BsaAI yAC'GTr

Cuts at:      0     129     408 
   Size:        129     279
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     130     408 
   Size:        130     278
 
BsiHKAI G_wGCw'C

Cuts at:      0     161     408 
   Size:        161     247
 
BsmI GAATG_Cn'

Cuts at:      0     306     408 
   Size:        306     102
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     312     408 
   Size:        312      96
 
Bsp1286I G_dGCh'C

Cuts at:      0      56     161     408 
   Size:         56     105     247

  Fragments arranged by size: 

                 247     105      56
 
BsrDI GCAATG_nn'

Cuts at:      0     371     408 
   Size:        371      37
 
BstYI r'GATC_y

Cuts at:      0     228     408 
   Size:        228     180
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0       6     408 
   Size:          6     402
 
EcoICRI GAG'CTC

Cuts at:      0     159     408 
   Size:        159     249
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     109     408 
   Size:        109     299
 
EcoO109I rG'GnC_Cy

Cuts at:      0      38      52     408 
   Size:         38      14     356

  Fragments arranged by size: 

                 356      38      14
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     260     292     408 
   Size:        260      32     116

  Fragments arranged by size: 

                 260     116      32
 
HincII GTy'rAC

Cuts at:      0     285     408 
   Size:        285     123
 
HpaI GTT'AAC

Cuts at:      0     285     408 
   Size:        285     123
 
MluI A'CGCG_T

Cuts at:      0     270     408 
   Size:        270     138
 
MspA1I CmG'CkG

Cuts at:      0      22     106     408 
   Size:         22      84     302

  Fragments arranged by size: 

                 302      84      22
 
PfoI T'CCnGG_A

Cuts at:      0     161     408 
   Size:        161     247
 
PpuMI rG'GwC_Cy

Cuts at:      0      38     408 
   Size:         38     370
 
PspOMI G'GGCC_C

Cuts at:      0      52     408 
   Size:         52     356
 
SacI G_AGCT'C

Cuts at:      0     161     408 
   Size:        161     247
 
SanDI GG'GwC_CC

Cuts at:      0      38     408 
   Size:         38     370
 
SfcI C'TryA_G

Cuts at:      0     295     408 
   Size:        295     113
 
StyI C'CwwG_G

Cuts at:      0     341     408 
   Size:        341      67
 

 Enzymes that do cut:

   AflIII     AhdI     ApaI     ApoI    BanII Bme1580I     BpmI   Bpu10I
    BsaAI     BsgI  BsiHKAI     BsmI    BsmBI Bsp1286I    BsrDI    BstYI
     EciI  EcoICRI  Eco57MI EcoO109I    Hin4I   HincII     HpaI     MluI
   MspA1I     PfoI    PpuMI   PspOMI     SacI    SanDI     SfcI     StyI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
     AgeI     AleI     AlfI     AloI    AlwNI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI     BbeI     BbsI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
    BmgBI     BmrI     BmtI     BplI    BpuEI     BsaI    BsaBI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI    BsiEI    BsiWI    BspEI    BspHI
    BspMI    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI
   BstEII    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI
    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EarI    EcoNI
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII  HindIII
     KasI     KpnI     MfeI     MmeI     MscI     MslI     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI    PflMI     PmeI     PmlI     PpiI    PshAI
     PsiI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII    SacII
     SalI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     SwaI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI
     XhoI     XmaI     XmnI     ZraI