(Linear) (Six-base) MAPSORT of: KIEE1620.seq  Check: 3619  from: 1  to: 396



KIEE1620.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:43  ..

Acc65I G'GTAC_C

Cuts at:      0      19     396 
   Size:         19     377
 
AflIII A'CryG_T

Cuts at:      0     279     396 
   Size:        279     117
 
ApoI r'AATT_y

Cuts at:      0     208     396 
   Size:        208     188
 
BanI G'GyrC_C

Cuts at:      0      19     396 
   Size:         19     377
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     205     239     396 
   Size:        205      34     157

  Fragments arranged by size: 

                 205     157      34
 
BclI T'GATC_A

Cuts at:      0     122     146     396 
   Size:        122      24     250

  Fragments arranged by size: 

                 250     122      24
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     208     396 
   Size:        208     188
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      21     396 
   Size:         21     375
 
BsaHI Gr'CG_yC

Cuts at:      0     365     396 
   Size:        365      31
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     304     396 
   Size:        304      92
 
BsiHKAI G_wGCw'C

Cuts at:      0     250     396 
   Size:        250     146
 
BsmI GAATG_Cn'

Cuts at:      0     355     396 
   Size:        355      41
 
Bsp1286I G_dGCh'C

Cuts at:      0     250     396 
   Size:        250     146
 
BspHI T'CATG_A

Cuts at:      0     356     396 
   Size:        356      40
 
BssSI C'ACGA_G

Cuts at:      0     375     396 
   Size:        375      21
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      96     396 
   Size:         96     300
 
BtsI GCAGTG_nn'

Cuts at:      0     221     396 
   Size:        221     175
 
DraI TTT'AAA

Cuts at:      0     174     396 
   Size:        174     222
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     238     396 
   Size:        238     158
 
EaeI y'GGCC_r

Cuts at:      0     107     396 
   Size:        107     289
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      21     396 
   Size:         21     375
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     286     318     396 
   Size:        286      32      78

  Fragments arranged by size: 

                 286      78      32
 
KpnI G_GTAC'C

Cuts at:      0      23     396 
   Size:         23     373
 
MscI TGG'CCA

Cuts at:      0     109     396 
   Size:        109     287
 
MslI CAynn'nnrTG

Cuts at:      0     130     396 
   Size:        130     266
 
NspI r_CATG'y

Cuts at:      0     283     396 
   Size:        283     113
 
PciI A'CATG_T

Cuts at:      0     279     396 
   Size:        279     117
 
PstI C_TGCA'G

Cuts at:      0     228     396 
   Size:        228     168
 
SfcI C'TryA_G

Cuts at:      0     224     396 
   Size:        224     172
 
Tth111I GACn'n_nGTC

Cuts at:      0     217     396 
   Size:        217     179
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      93     396 
   Size:         93     303
 

 Enzymes that do cut:

   Acc65I   AflIII     ApoI     BanI     BcgI     BclI     BmrI     BpmI
    BsaHI     BsgI  BsiHKAI     BsmI Bsp1286I    BspHI    BssSI    BstXI
     BtsI     DraI     DrdI     EaeI  Eco57MI    Hin4I     KpnI     MscI
     MslI     NspI     PciI     PstI     SfcI  Tth111I     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AcuI     AfeI    AflII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI    BanII     BbeI
     BbsI    BbvCI    BciVI    BfrBI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaWI    BsaXI    BseRI    BseYI    BsiEI    BsiWI    BsmBI    BspEI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII   BstAPI    BstBI
   BstEII    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI    CspCI
   DraIII     EagI     EarI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI    HaeII   HincII  HindIII
     HpaI     KasI     MfeI     MluI     MmeI   MspA1I     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI
     PasI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     StyI     SwaI    TaqII     TatI     TstI
     XbaI     XhoI     XmaI     XmnI     ZraI