(Linear) (Six-base) MAPSORT of: KIEE1616.seq  Check: 1018  from: 1  to: 381



KIEE1616.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:43  ..

BglI GCCn_nnn'nGGC

Cuts at:      0      25     381 
   Size:         25     356
 
Bme1580I G_kGCm'C

Cuts at:      0     339     381 
   Size:        339      42
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      66     381 
   Size:         66     315
 
Bsp1286I G_dGCh'C

Cuts at:      0     339     381 
   Size:        339      42
 
BsrDI GCAATG_nn'

Cuts at:      0      74     381 
   Size:         74     307
 
BtgI C'CryG_G

Cuts at:      0     312     381 
   Size:        312      69
 
BtsI GCAGTG_nn'

Cuts at:      0     253     381 
   Size:        253     128
 
EaeI y'GGCC_r

Cuts at:      0     363     381 
   Size:        363      18
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      66     381 
   Size:         66     315
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0      85     381 
   Size:         85     296
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0       5      37     381 
   Size:          5      32     344

  Fragments arranged by size: 

                 344      32       5
 
MspA1I CmG'CkG

Cuts at:      0      86     381 
   Size:         86     295
 
NcoI C'CATG_G

Cuts at:      0     312     381 
   Size:        312      69
 
NspI r_CATG'y

Cuts at:      0      55     381 
   Size:         55     326
 
PfoI T'CCnGG_A

Cuts at:      0     125     381 
   Size:        125     256
 
PvuII CAG'CTG

Cuts at:      0      86     381 
   Size:         86     295
 
StyI C'CwwG_G

Cuts at:      0      29     312     381 
   Size:         29     283      69

  Fragments arranged by size: 

                 283      69      29
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      27     123     160     381 
   Size:         27      96      37     221

  Fragments arranged by size: 

                 221      96      37      27
 

 Enzymes that do cut:

     BglI Bme1580I     BpmI Bsp1286I    BsrDI     BtgI     BtsI     EaeI
  Eco57MI     FalI    Hin4I   MspA1I     NcoI     NspI     PfoI    PvuII
     StyI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI
    BamHI     BanI    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI
     BclI    BfrBI    BglII     BlpI    BmgBI     BmrI     BmtI     BplI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI
    BspEI    BspHI    BspMI    BsrBI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I    BtgZI
     ClaI    CspCI     DraI   DraIII     DrdI     EagI     EarI     EciI
  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FseI     FspI    FspAI
    HaeII   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     MscI     MslI     NaeI     NarI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     PacI     PasI     PciI    PflMI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PstI     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     SwaI    TaqII     TatI     TstI  Tth111I     XbaI     XhoI     XmaI
     XmnI     ZraI