(Linear) (Six-base) MAPSORT of: KIEE1615.seq  Check: 7218  from: 1  to: 378



KIEE1615.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:43  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     157     378 
   Size:        157     221
 
AfeI AGC'GCT

Cuts at:      0     259     378 
   Size:        259     119
 
AflIII A'CryG_T

Cuts at:      0     122     378 
   Size:        122     256
 
BanI G'GyrC_C

Cuts at:      0     343     378 
   Size:        343      35
 
BanII G_rGCy'C

Cuts at:      0      43     378 
   Size:         43     335
 
Bme1580I G_kGCm'C

Cuts at:      0     346     378 
   Size:        346      32
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     129     378 
   Size:        129     249
 
BsaAI yAC'GTr

Cuts at:      0     123     378 
   Size:        123     255
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     244     274     355     378 
   Size:        244      30      81      23

  Fragments arranged by size: 

                 244      81      30      23
 
BsiEI CG_ry'CG

Cuts at:      0     293     378 
   Size:        293      85
 
Bsp1286I G_dGCh'C

Cuts at:      0      43     346     378 
   Size:         43     303      32

  Fragments arranged by size: 

                 303      43      32
 
BspHI T'CATG_A

Cuts at:      0     185     378 
   Size:        185     193
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     132     378 
   Size:        132     246
 
BsrBI CCG'CTC

Cuts at:      0      22     378 
   Size:         22     356
 
BsrFI r'CCGG_y

Cuts at:      0       9     157     378 
   Size:          9     148     221

  Fragments arranged by size: 

                 221     148       9
 
BsrGI T'GTAC_A

Cuts at:      0     125     378 
   Size:        125     253
 
BstEII G'GTnAC_C

Cuts at:      0     354     378 
   Size:        354      24
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     177     378 
   Size:        177     201
 
BstYI r'GATC_y

Cuts at:      0     281     378 
   Size:        281      97
 
BtgI C'CryG_G

Cuts at:      0     176     378 
   Size:        176     202
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     204     378 
   Size:        204     174
 
DraIII CAC_nnn'GTG

Cuts at:      0     333     378 
   Size:        333      45
 
EaeI y'GGCC_r

Cuts at:      0     290     320     378 
   Size:        290      30      58

  Fragments arranged by size: 

                 290      58      30
 
EagI C'GGCC_G

Cuts at:      0     290     378 
   Size:        290      88
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     157     378 
   Size:        157     221
 
HaeII r_GCGC'y

Cuts at:      0     261     378 
   Size:        261     117
 
HincII GTy'rAC

Cuts at:      0     201     378 
   Size:        201     177
 
MscI TGG'CCA

Cuts at:      0     322     378 
   Size:        322      56
 
MslI CAynn'nnrTG

Cuts at:      0     121     378 
   Size:        121     257
 
NcoI C'CATG_G

Cuts at:      0     176     378 
   Size:        176     202
 
StyI C'CwwG_G

Cuts at:      0     170     176     347     378 
   Size:        170       6     171      31

  Fragments arranged by size: 

                 171     170      31       6
 
TatI w'GTAC_w

Cuts at:      0     125     362     378 
   Size:        125     237      16

  Fragments arranged by size: 

                 237     125      16
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     319     351     378 
   Size:        319      32      27

  Fragments arranged by size: 

                 319      32      27
 
XmnI GAAnn'nnTTC

Cuts at:      0     193     378 
   Size:        193     185
 

 Enzymes that do cut:

     AcuI     AfeI   AflIII     BanI    BanII Bme1580I     BplI    BsaAI
    BsaXI    BsiEI Bsp1286I    BspHI    BspMI    BsrBI    BsrFI    BsrGI
   BstEII    BstXI    BstYI     BtgI    BtgZI   DraIII     EaeI     EagI
  Eco57MI    HaeII   HincII     MscI     MslI     NcoI     StyI     TatI
     TstI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI    AflII     AgeI     AhdI
     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BbeI     BbsI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
    BmgBI     BmrI     BmtI     BpmI   Bpu10I    BpuEI     BsaI    BsaBI
    BsaHI    BsaWI    BseRI    BseYI     BsgI  BsiHKAI    BsiWI     BsmI
    BsmBI    BspEI    BsrDI   BssHII    BssSI   BstAPI    BstBI  BstZ17I
   Bsu36I     BtsI     ClaI    CspCI     DraI     DrdI     EarI     EciI
  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI    Hin4I  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI   MspA1I     NaeI     NarI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     SwaI    TaqII  Tth111I     XbaI     XcmI     XhoI
     XmaI     ZraI