(Linear) (Six-base) MAPSORT of: KIEE1613.seq  Check: 2938  from: 1  to: 375



KIEE1613.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:43  ..

AclI AA'CG_TT

Cuts at:      0     351     375 
   Size:        351      24
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     220     375 
   Size:        220     155
 
AflIII A'CryG_T

Cuts at:      0     217     375 
   Size:        217     158
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      55     375 
   Size:         55     320
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     373     375 
   Size:        373       2
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      64     375 
   Size:         64     311
 
BsrDI GCAATG_nn'

Cuts at:      0     188     375 
   Size:        188     187
 
BstBI TT'CG_AA

Cuts at:      0     339     375 
   Size:        339      36
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     220     375 
   Size:        220     155
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      69     101     375 
   Size:         69      32     274

  Fragments arranged by size: 

                 274      69      32
 
NspI r_CATG'y

Cuts at:      0     221     375 
   Size:        221     154
 
PciI A'CATG_T

Cuts at:      0     217     375 
   Size:        217     158
 
StyI C'CwwG_G

Cuts at:      0     111     375 
   Size:        111     264
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     115     147     375 
   Size:        115      32     228

  Fragments arranged by size: 

                 228     115      32
 

 Enzymes that do cut:

     AclI     AcuI   AflIII     AlfI     BbsI     BmrI    BsrDI    BstBI
  Eco57MI    Hin4I     NspI     PciI     StyI     TstI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AfeI    AflII     AgeI     AhdI
     AleI     AloI    AlwNI     ApaI    ApaLI     ApoI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI
    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI   BstEII
    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI
    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EarI     EciI
  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI    HaeII   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI
    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     SwaI    TaqII     TatI  Tth111I
     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI