(Linear) (Six-base) MAPSORT of: KIEE1612.seq  Check: 6515  from: 1  to: 357



KIEE1612.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:43  ..

AlwNI CAG_nnn'CTG

Cuts at:      0     310     357 
   Size:        310      47
 
ApaI G_GGCC'C

Cuts at:      0     322     357 
   Size:        322      35
 
ApoI r'AATT_y

Cuts at:      0     122     357 
   Size:        122     235
 
AvrII C'CTAG_G

Cuts at:      0     292     357 
   Size:        292      65
 
BanII G_rGCy'C

Cuts at:      0      76     322     357 
   Size:         76     246      35

  Fragments arranged by size: 

                 246      76      35
 
Bme1580I G_kGCm'C

Cuts at:      0      54     322     357 
   Size:         54     268      35

  Fragments arranged by size: 

                 268      54      35
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     160     357 
   Size:        160     197
 
BsaHI Gr'CG_yC

Cuts at:      0     269     357 
   Size:        269      88
 
BsiHKAI G_wGCw'C

Cuts at:      0     169     357 
   Size:        169     188
 
Bsp1286I G_dGCh'C

Cuts at:      0      54      76     169     322     357 
   Size:         54      22      93     153      35

  Fragments arranged by size: 

                 153      93      54      35      22
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     306     357 
   Size:        306      51
 
BtsI GCAGTG_nn'

Cuts at:      0     311     357 
   Size:        311      46
 
EcoO109I rG'GnC_Cy

Cuts at:      0     318     319     357 
   Size:        318       1      38

  Fragments arranged by size: 

                 318      38       1
 
EcoRI G'AATT_C

Cuts at:      0     122     357 
   Size:        122     235
 
EcoRV GAT'ATC

Cuts at:      0     334     357 
   Size:        334      23
 
MspA1I CmG'CkG

Cuts at:      0     153     357 
   Size:        153     204
 
NspI r_CATG'y

Cuts at:      0     105     173     357 
   Size:        105      68     184

  Fragments arranged by size: 

                 184     105      68
 
PspOMI G'GGCC_C

Cuts at:      0     318     357 
   Size:        318      39
 
PvuII CAG'CTG

Cuts at:      0     153     357 
   Size:        153     204
 
SmlI C'TyrA_G

Cuts at:      0     175     357 
   Size:        175     182
 
StuI AGG'CCT

Cuts at:      0       8     357 
   Size:          8     349
 
StyI C'CwwG_G

Cuts at:      0      39     292     357 
   Size:         39     253      65

  Fragments arranged by size: 

                 253      65      39
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     217     334     357 
   Size:        217     117      23

  Fragments arranged by size: 

                 217     117      23
 

 Enzymes that do cut:

    AlwNI     ApaI     ApoI    AvrII    BanII Bme1580I    BpuEI    BsaHI
  BsiHKAI Bsp1286I    BstXI     BtsI EcoO109I    EcoRI    EcoRV   MspA1I
     NspI   PspOMI    PvuII     SmlI     StuI     StyI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    ApaLI     AscI
     AseI    AsiSI     AvaI     BaeI    BamHI     BanI     BbeI     BbsI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I     BsaI    BsaAI
    BsaBI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI    BsiWI
     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstYI  BstZ17I
   Bsu36I     BtgI    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI
     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI     FalI
     FseI     FspI    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI     MscI     MslI     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI    PspXI     PsrI     PstI     PvuI    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SphI     SrfI     SspI     SwaI    TaqII     TatI     TstI  Tth111I
     XbaI     XhoI     XmaI     XmnI     ZraI