(Linear) (Six-base) MAPSORT of: KIEE1609.seq  Check: 2488  from: 1  to: 342



KIEE1609.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:43  ..

AccI GT'mk_AC

Cuts at:      0     171     342 
   Size:        171     171
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     274     342 
   Size:        274      68
 
BstEII G'GTnAC_C

Cuts at:      0     184     342 
   Size:        184     158
 
BtsI GCAGTG_nn'

Cuts at:      0     275     342 
   Size:        275      67
 
EarI CTCTTCn'nnn_

Cuts at:      0     122     342 
   Size:        122     220
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0      85     342 
   Size:         85     257
 
HincII GTy'rAC

Cuts at:      0     172     342 
   Size:        172     170
 
SalI G'TCGA_C

Cuts at:      0     170     342 
   Size:        170     172
 
SexAI A'CCwGG_T

Cuts at:      0     300     342 
   Size:        300      42
 
SfcI C'TryA_G

Cuts at:      0      38     342 
   Size:         38     304
 

 Enzymes that do cut:

     AccI   BstAPI   BstEII     BtsI     EarI     FalI   HincII     SalI
    SexAI     SfcI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
     BanI    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI
    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI
     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI    BstBI    BstXI    BstYI  BstZ17I
   Bsu36I     BtgI    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI
     EaeI     EagI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI
    EcoRV     FseI     FspI    FspAI    HaeII    Hin4I  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI     MscI     MslI   MspA1I
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PstI     PvuI    PvuII    RsrII     SacI    SacII    SanDI     SapI
     SbfI     ScaI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI     SwaI
    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI
     XmnI     ZraI