(Linear) (Six-base) MAPSORT of: KIEE1608.seq  Check: 1575  from: 1  to: 342



KIEE1608.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:43  ..

AleI CACnn'nnGTG

Cuts at:      0     287     342 
   Size:        287      55
 
ApaI G_GGCC'C

Cuts at:      0     301     342 
   Size:        301      41
 
ApoI r'AATT_y

Cuts at:      0     106     138     342 
   Size:        106      32     204

  Fragments arranged by size: 

                 204     106      32
 
AvaI C'yCGr_G

Cuts at:      0     325     342 
   Size:        325      17
 
BanI G'GyrC_C

Cuts at:      0      80     334     342 
   Size:         80     254       8

  Fragments arranged by size: 

                 254      80       8
 
BanII G_rGCy'C

Cuts at:      0     301     342 
   Size:        301      41
 
BglII A'GATC_T

Cuts at:      0     129     342 
   Size:        129     213
 
BlpI GC'TnA_GC

Cuts at:      0     220     342 
   Size:        220     122
 
Bme1580I G_kGCm'C

Cuts at:      0      83     301     337     342 
   Size:         83     218      36       5

  Fragments arranged by size: 

                 218      83      36       5
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     130     342 
   Size:        130     212
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      31     342 
   Size:         31     311
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     244     342 
   Size:        244      98
 
BseYI C'CCAG_C

Cuts at:      0      75     342 
   Size:         75     267
 
Bsp1286I G_dGCh'C

Cuts at:      0      83     301     337     342 
   Size:         83     218      36       5

  Fragments arranged by size: 

                 218      83      36       5
 
BssSI C'ACGA_G

Cuts at:      0     271     283     342 
   Size:        271      12      59

  Fragments arranged by size: 

                 271      59      12
 
BstYI r'GATC_y

Cuts at:      0     129     342 
   Size:        129     213
 
EaeI y'GGCC_r

Cuts at:      0     303     342 
   Size:        303      39
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      97     328     342 
   Size:         97     231      14

  Fragments arranged by size: 

                 231      97      14
 
EcoO109I rG'GnC_Cy

Cuts at:      0     192     297     298     342 
   Size:        192     105       1      44

  Fragments arranged by size: 

                 192     105      44       1
 
EcoRI G'AATT_C

Cuts at:      0     106     342 
   Size:        106     236
 
MscI TGG'CCA

Cuts at:      0     305     342 
   Size:        305      37
 
MslI CAynn'nnrTG

Cuts at:      0     244     287     342 
   Size:        244      43      55

  Fragments arranged by size: 

                 244      55      43
 
MspA1I CmG'CkG

Cuts at:      0      21     219     342 
   Size:         21     198     123

  Fragments arranged by size: 

                 198     123      21
 
PpuMI rG'GwC_Cy

Cuts at:      0     192     342 
   Size:        192     150
 
PspOMI G'GGCC_C

Cuts at:      0     297     342 
   Size:        297      45
 
PvuII CAG'CTG

Cuts at:      0      21     219     342 
   Size:         21     198     123

  Fragments arranged by size: 

                 198     123      21
 
SmlI C'TyrA_G

Cuts at:      0     145     342 
   Size:        145     197
 
StuI AGG'CCT

Cuts at:      0     323     342 
   Size:        323      19
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      98     342 
   Size:         98     244
 

 Enzymes that do cut:

     AleI     ApaI     ApoI     AvaI     BanI    BanII    BglII     BlpI
 Bme1580I    BpuEI     BsaI    BseRI    BseYI Bsp1286I    BssSI    BstYI
     EaeI    EcoNI EcoO109I    EcoRI     MscI     MslI   MspA1I    PpuMI
   PspOMI    PvuII     SmlI     StuI    TaqII

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AlfI     AloI    AlwNI    ApaLI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI     BbeI     BbsI    BbvCI
     BcgI    BciVI     BclI    BfrBI     BglI    BmgBI     BmrI     BmtI
     BplI     BpmI   Bpu10I    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII   BstAPI    BstBI
   BstEII    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI
    CspCI     DraI   DraIII     DrdI     EagI     EarI     EciI  EcoICRI
  Eco57MI    EcoRV     FalI     FseI     FspI    FspAI    HaeII    Hin4I
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI
     PmlI     PpiI    PshAI     PsiI    PspXI     PsrI     PstI     PvuI
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SphI     SrfI     SspI     StyI     SwaI     TatI     TstI
  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI