(Linear) (Six-base) MAPSORT of: KIEE1607.seq  Check: 2574  from: 1  to: 342



KIEE1607.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:43  ..

AleI CACnn'nnGTG

Cuts at:      0     226     342 
   Size:        226     116
 
AlwNI CAG_nnn'CTG

Cuts at:      0     117     342 
   Size:        117     225
 
BmgBI CAC'GTC

Cuts at:      0     237     342 
   Size:        237     105
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     183     342 
   Size:        183     159
 
BsaAI yAC'GTr

Cuts at:      0     224     342 
   Size:        224     118
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      78     108     342 
   Size:         78      30     234

  Fragments arranged by size: 

                 234      78      30
 
BseYI C'CCAG_C

Cuts at:      0     110     305     342 
   Size:        110     195      37

  Fragments arranged by size: 

                 195     110      37
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     227     342 
   Size:        227     115
 
BtsI GCAGTG_nn'

Cuts at:      0      41     342 
   Size:         41     301
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     205     240     342 
   Size:        205      35     102

  Fragments arranged by size: 

                 205     102      35
 
EcoO109I rG'GnC_Cy

Cuts at:      0      89     342 
   Size:         89     253
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      28     224     244     256     276     342 
   Size:         28     196      20      12      20      66

  Fragments arranged by size: 

                 196      66      28      20      20      12
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     287     342 
   Size:        287      55
 
MslI CAynn'nnrTG

Cuts at:      0     226     342 
   Size:        226     116
 
MspA1I CmG'CkG

Cuts at:      0     325     342 
   Size:        325      17
 
PmlI CAC'GTG

Cuts at:      0     224     342 
   Size:        224     118
 
PpuMI rG'GwC_Cy

Cuts at:      0      89     342 
   Size:         89     253
 
PvuII CAG'CTG

Cuts at:      0     325     342 
   Size:        325      17
 
SmlI C'TyrA_G

Cuts at:      0     162     342 
   Size:        162     180
 
StyI C'CwwG_G

Cuts at:      0      24     342 
   Size:         24     318
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     216     342 
   Size:        216     126
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      78     110     342 
   Size:         78      32     232

  Fragments arranged by size: 

                 232      78      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     238     342 
   Size:        238     104
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     221     342 
   Size:        221     121
 

 Enzymes that do cut:

     AleI    AlwNI    BmgBI    BpuEI    BsaAI    BsaXI    BseYI    BsmBI
     BtsI    CspCI EcoO109I    Hin4I     MmeI     MslI   MspA1I     PmlI
    PpuMI    PvuII     SmlI     StyI    TaqII     TstI  Tth111I     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AlfI     AloI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI
     BglI    BglII     BlpI Bme1580I     BmrI     BmtI     BplI     BpmI
   Bpu10I     BsaI    BsaBI    BsaHI    BsaWI    BseRI     BsgI    BsiEI
  BsiHKAI    BsiWI     BsmI Bsp1286I    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI     DraI
   DraIII     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI
    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MscI
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI
     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     SwaI     TatI     XbaI
     XhoI     XmaI     XmnI     ZraI