(Linear) (Six-base) MAPSORT of: KIEE1606.seq  Check: 799  from: 1  to: 336



KIEE1606.orf

 With 182 enzymes: SgfI * 

                        July 3, 2012 16:57  ..

AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      46     336 
   Size:         46     290
 
AlwNI CAG_nnn'CTG

Cuts at:      0     227     336 
   Size:        227     109
 
ApaI G_GGCC'C

Cuts at:      0      11     336 
   Size:         11     325
 
ApoI r'AATT_y

Cuts at:      0     309     336 
   Size:        309      27
 
BanII G_rGCy'C

Cuts at:      0      11      55     336 
   Size:         11      44     281

  Fragments arranged by size: 

                 281      44      11
 
Bme1580I G_kGCm'C

Cuts at:      0      11     336 
   Size:         11     325
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     175     336 
   Size:        175     161
 
Bpu10I CC'TnA_GC

Cuts at:      0      49     322     336 
   Size:         49     273      14

  Fragments arranged by size: 

                 273      49      14
 
BseYI C'CCAG_C

Cuts at:      0      85     247     336 
   Size:         85     162      89

  Fragments arranged by size: 

                 162      89      85
 
BsiHKAI G_wGCw'C

Cuts at:      0     272     336 
   Size:        272      64
 
BsmI GAATG_Cn'

Cuts at:      0      70     336 
   Size:         70     266
 
Bsp1286I G_dGCh'C

Cuts at:      0      11      55     272     336 
   Size:         11      44     217      64

  Fragments arranged by size: 

                 217      64      44      11
 
BsrDI GCAATG_nn'

Cuts at:      0      40     336 
   Size:         40     296
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      95     227     336 
   Size:         95     132     109

  Fragments arranged by size: 

                 132     109      95
 
BtgI C'CryG_G

Cuts at:      0     288     336 
   Size:        288      48
 
BtsI GCAGTG_nn'

Cuts at:      0      89     336 
   Size:         89     247
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     175     336 
   Size:        175     161
 
EcoO109I rG'GnC_Cy

Cuts at:      0       7       8     336 
   Size:          7       1     328

  Fragments arranged by size: 

                 328       7       1
 
MslI CAynn'nnrTG

Cuts at:      0     154     336 
   Size:        154     182
 
NcoI C'CATG_G

Cuts at:      0     288     336 
   Size:        288      48
 
PspOMI G'GGCC_C

Cuts at:      0       7     336 
   Size:          7     329
 
PstI C_TGCA'G

Cuts at:      0      25     167     336 
   Size:         25     142     169

  Fragments arranged by size: 

                 169     142      25
 
SfcI C'TryA_G

Cuts at:      0      21      62     163     336 
   Size:         21      41     101     173

  Fragments arranged by size: 

                 173     101      41      21
 
StyI C'CwwG_G

Cuts at:      0     288     336 
   Size:        288      48
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      99     336 
   Size:         99     237
 
TatI w'GTAC_w

Cuts at:      0     100     336 
   Size:        100     236
 

 Enzymes that do cut:

     AlfI    AlwNI     ApaI     ApoI    BanII Bme1580I     BpmI   Bpu10I
    BseYI  BsiHKAI     BsmI Bsp1286I    BsrDI   BstAPI     BtgI     BtsI
  Eco57MI EcoO109I     MslI     NcoI   PspOMI     PstI     SfcI     StyI
    TaqII     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AloI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI     BbeI     BbsI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
    BmgBI     BmrI     BmtI     BplI    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI     BsgI    BsiEI    BsiWI    BsmBI
    BspEI    BspHI    BspMI    BsrBI    BsrFI    BsrGI   BssHII    BssSI
    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I    BtgZI     ClaI
    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EarI     EciI
  EcoICRI    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
    HaeII    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MmeI     MscI   MspA1I     NaeI     NarI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
    PspXI     PsrI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     SwaI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI
     XmnI     ZraI