(Linear) (Six-base) MAPSORT of: KIEE1604.seq  Check: 4590  from: 1  to: 321



KIEE1604.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:43  ..

AlwNI CAG_nnn'CTG

Cuts at:      0     144     321 
   Size:        144     177
 
ApoI r'AATT_y

Cuts at:      0     152     321 
   Size:        152     169
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     145     217     321 
   Size:        145      72     104

  Fragments arranged by size: 

                 145     104      72
 
BseYI C'CCAG_C

Cuts at:      0      28     321 
   Size:         28     293
 
BsrFI r'CCGG_y

Cuts at:      0      86     321 
   Size:         86     235
 
BssHII G'CGCG_C

Cuts at:      0      90     321 
   Size:         90     231
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     144     321 
   Size:        144     177
 
BtgI C'CryG_G

Cuts at:      0     272     321 
   Size:        272      49
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     251     321 
   Size:        251      70
 
BtsI GCAGTG_nn'

Cuts at:      0     201     321 
   Size:        201     120
 
EaeI y'GGCC_r

Cuts at:      0      77     110     321 
   Size:         77      33     211

  Fragments arranged by size: 

                 211      77      33
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0       9     321 
   Size:          9     312
 
EcoRI G'AATT_C

Cuts at:      0     152     321 
   Size:        152     169
 
HaeII r_GCGC'y

Cuts at:      0      58     106     321 
   Size:         58      48     215

  Fragments arranged by size: 

                 215      58      48
 
HindIII A'AGCT_T

Cuts at:      0     248     321 
   Size:        248      73
 
MscI TGG'CCA

Cuts at:      0     112     321 
   Size:        112     209
 
NaeI GCC'GGC

Cuts at:      0      88     321 
   Size:         88     233
 
NcoI C'CATG_G

Cuts at:      0     272     321 
   Size:        272      49
 
NgoMIV G'CCGG_C

Cuts at:      0      86     321 
   Size:         86     235
 
PstI C_TGCA'G

Cuts at:      0     149     321 
   Size:        149     172
 
SbfI CC_TGCA'GG

Cuts at:      0     149     321 
   Size:        149     172
 
SfcI C'TryA_G

Cuts at:      0     145     321 
   Size:        145     176
 
SmlI C'TyrA_G

Cuts at:      0     160     232     321 
   Size:        160      72      89

  Fragments arranged by size: 

                 160      89      72
 
StyI C'CwwG_G

Cuts at:      0     272     321 
   Size:        272      49
 

 Enzymes that do cut:

    AlwNI     ApoI    BpuEI    BseYI    BsrFI   BssHII   BstAPI     BtgI
    BtgZI     BtsI     EaeI     EciI    EcoRI    HaeII  HindIII     MscI
     NaeI     NcoI   NgoMIV     PstI     SbfI     SfcI     SmlI     StyI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI
     BpmI   Bpu10I     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrGI    BssSI    BstBI
   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     ClaI    CspCI     DraI
   DraIII     DrdI     EagI     EarI  EcoICRI  Eco57MI    EcoNI EcoO109I
    EcoRV     FalI     FseI     FspI    FspAI    Hin4I   HincII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI     MslI   MspA1I     NarI
     NdeI     NheI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SapI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     SwaI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI
     XhoI     XmaI     XmnI     ZraI