(Linear) (Six-base) MAPSORT of: KIEE1602.seq  Check: 1602  from: 1  to: 309



KIEE1602.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:43  ..

AccI GT'mk_AC

Cuts at:      0     263     309 
   Size:        263      46
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      82     309 
   Size:         82     227
 
BanI G'GyrC_C

Cuts at:      0      43     107     309 
   Size:         43      64     202

  Fragments arranged by size: 

                 202      64      43
 
BbeI G_GCGC'C

Cuts at:      0     111     309 
   Size:        111     198
 
BglI GCCn_nnn'nGGC

Cuts at:      0     125     309 
   Size:        125     184
 
BmgBI CAC'GTC

Cuts at:      0     258     309 
   Size:        258      51
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     208     309 
   Size:        208     101
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     256     309 
   Size:        256      53
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     154     309 
   Size:        154     155
 
BsaHI Gr'CG_yC

Cuts at:      0     108     206     309 
   Size:        108      98     103

  Fragments arranged by size: 

                 108     103      98
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     172     309 
   Size:        172     137
 
BsiHKAI G_wGCw'C

Cuts at:      0     227     309 
   Size:        227      82
 
Bsp1286I G_dGCh'C

Cuts at:      0     227     309 
   Size:        227      82
 
BstEII G'GTnAC_C

Cuts at:      0     243     309 
   Size:        243      66
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     258     309 
   Size:        258      51
 
BtgI C'CryG_G

Cuts at:      0     120     165     251     309 
   Size:        120      45      86      58

  Fragments arranged by size: 

                 120      86      58      45
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     208     309 
   Size:        208     101
 
EcoO109I rG'GnC_Cy

Cuts at:      0      27     309 
   Size:         27     282
 
HaeII r_GCGC'y

Cuts at:      0      54     111     279     309 
   Size:         54      57     168      30

  Fragments arranged by size: 

                 168      57      54      30
 
KasI G'GCGC_C

Cuts at:      0     107     309 
   Size:        107     202
 
MslI CAynn'nnrTG

Cuts at:      0     250     256     309 
   Size:        250       6      53

  Fragments arranged by size: 

                 250      53       6
 
MspA1I CmG'CkG

Cuts at:      0     122     167     309 
   Size:        122      45     142

  Fragments arranged by size: 

                 142     122      45
 
NarI GG'CG_CC

Cuts at:      0     108     309 
   Size:        108     201
 
NcoI C'CATG_G

Cuts at:      0     251     309 
   Size:        251      58
 
PfoI T'CCnGG_A

Cuts at:      0     185     200     309 
   Size:        185      15     109

  Fragments arranged by size: 

                 185     109      15
 
SacII CC_GC'GG

Cuts at:      0     123     168     309 
   Size:        123      45     141

  Fragments arranged by size: 

                 141     123      45
 
SfoI GGC'GCC

Cuts at:      0     109     309 
   Size:        109     200
 
SmlI C'TyrA_G

Cuts at:      0     271     309 
   Size:        271      38
 
StyI C'CwwG_G

Cuts at:      0     251     309 
   Size:        251      58
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      27      59     309 
   Size:         27      32     250

  Fragments arranged by size: 

                 250      32      27
 
Tth111I GACn'n_nGTC

Cuts at:      0     259     309 
   Size:        259      50
 
XmnI GAAnn'nnTTC

Cuts at:      0     181     309 
   Size:        181     128
 

 Enzymes that do cut:

     AccI     AlfI     BanI     BbeI     BglI    BmgBI     BpmI    BpuEI
     BsaI    BsaHI    BseRI  BsiHKAI Bsp1286I   BstEII    BstXI     BtgI
  Eco57MI EcoO109I    HaeII     KasI     MslI   MspA1I     NarI     NcoI
     PfoI    SacII     SfoI     SmlI     StyI     TstI  Tth111I     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AloI    AlwNI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI    BanII
     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI    BglII     BlpI
 Bme1580I     BmrI     BmtI     BplI   Bpu10I    BsaAI    BsaBI    BsaWI
    BsaXI    BseYI     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI    BstYI  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI
    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EarI     EciI
  EcoICRI    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
    Hin4I   HincII  HindIII     HpaI     KpnI     MfeI     MluI     MmeI
     MscI     NaeI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI    PflMI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    PvuII    RsrII     SacI     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfcI     SfiI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     SwaI    TaqII     TatI     XbaI
     XcmI     XhoI     XmaI     ZraI