(Linear) (Six-base) MAPSORT of: KIEE1595.seq  Check: 7430  from: 1  to: 1011



KIEE1595.orf

 With 182 enzymes: SgfI * 

                        March 22, 2012 15:51  ..

ApaLI G'TGCA_C

Cuts at:      0     654    1011 
   Size:        654     357
 
ApoI r'AATT_y

Cuts at:      0     953    1011 
   Size:        953      58
 
BanI G'GyrC_C

Cuts at:      0      55     345     394    1011 
   Size:         55     290      49     617

  Fragments arranged by size: 

                 617     290      55      49
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1009    1011 
   Size:       1009       2
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     419     510    1011 
   Size:        419      91     501

  Fragments arranged by size: 

                 501     419      91
 
BclI T'GATC_A

Cuts at:      0     878    1011 
   Size:        878     133
 
Bme1580I G_kGCm'C

Cuts at:      0     397     555     658    1011 
   Size:        397     158     103     353

  Fragments arranged by size: 

                 397     353     158     103
 
Bpu10I CC'TnA_GC

Cuts at:      0      32    1011 
   Size:         32     979
 
BseYI C'CCAG_C

Cuts at:      0     314    1011 
   Size:        314     697
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     365    1011 
   Size:        365     646
 
BsiHKAI G_wGCw'C

Cuts at:      0     658     818    1011 
   Size:        658     160     193

  Fragments arranged by size: 

                 658     193     160
 
Bsp1286I G_dGCh'C

Cuts at:      0     397     555     658     818    1011 
   Size:        397     158     103     160     193

  Fragments arranged by size: 

                 397     193     160     158     103
 
BspHI T'CATG_A

Cuts at:      0     596    1011 
   Size:        596     415
 
BsrDI GCAATG_nn'

Cuts at:      0     539    1011 
   Size:        539     472
 
BsrGI T'GTAC_A

Cuts at:      0     714    1011 
   Size:        714     297
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     550    1011 
   Size:        550     461
 
Bsu36I CC'TnA_GG

Cuts at:      0      50     336    1011 
   Size:         50     286     675

  Fragments arranged by size: 

                 675     286      50
 
BtgI C'CryG_G

Cuts at:      0     620    1011 
   Size:        620     391
 
BtsI GCAGTG_nn'

Cuts at:      0     287    1011 
   Size:        287     724
 
DraIII CAC_nnn'GTG

Cuts at:      0     550    1011 
   Size:        550     461
 
EarI CTCTTCn'nnn_

Cuts at:      0     152     361    1011 
   Size:        152     209     650

  Fragments arranged by size: 

                 650     209     152
 
HincII GTy'rAC

Cuts at:      0     308    1011 
   Size:        308     703
 
MspA1I CmG'CkG

Cuts at:      0     289     486    1011 
   Size:        289     197     525

  Fragments arranged by size: 

                 525     289     197
 
PfoI T'CCnGG_A

Cuts at:      0      66    1011 
   Size:         66     945
 
PstI C_TGCA'G

Cuts at:      0     294    1011 
   Size:        294     717
 
PvuII CAG'CTG

Cuts at:      0     289     486    1011 
   Size:        289     197     525

  Fragments arranged by size: 

                 525     289     197
 
SapI GCTCTTCn'nnn_

Cuts at:      0     152     361    1011 
   Size:        152     209     650

  Fragments arranged by size: 

                 650     209     152
 
SfcI C'TryA_G

Cuts at:      0     290     870    1011 
   Size:        290     580     141

  Fragments arranged by size: 

                 580     290     141
 
SspI AAT'ATT

Cuts at:      0     495    1011 
   Size:        495     516
 
StuI AGG'CCT

Cuts at:      0     809    1011 
   Size:        809     202
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     835    1011 
   Size:        835     176
 
TatI w'GTAC_w

Cuts at:      0     714    1011 
   Size:        714     297
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     341     373    1011 
   Size:        341      32     638

  Fragments arranged by size: 

                 638     341      32
 

 Enzymes that do cut:

    ApaLI     ApoI     BanI     BbsI    BciVI     BclI Bme1580I   Bpu10I
    BseYI     BsgI  BsiHKAI Bsp1286I    BspHI    BsrDI    BsrGI   BstAPI
   Bsu36I     BtgI     BtsI   DraIII     EarI   HincII   MspA1I     PfoI
     PstI    PvuII     SapI     SfcI     SspI     StuI    TaqII     TatI
     TstI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI    BanII
     BbeI    BbvCI     BcgI    BfrBI     BglI    BglII     BlpI    BmgBI
     BmrI     BmtI     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI    BsiEI    BsiWI     BsmI    BsmBI
    BspEI    BspMI    BsrBI    BsrFI   BssHII    BssSI    BstBI   BstEII
    BstXI    BstYI  BstZ17I    BtgZI     ClaI    CspCI     DraI     DrdI
     EaeI     EagI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI    HaeII    Hin4I  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI     MslI
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI    PflMI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI
    RsrII     SacI    SacII     SalI    SanDI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     StyI     SwaI  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI