Restriction map for
pFN21AE1591
(Linear) (Six-base) MAPSORT of: KIEE1591.seq Check: 2911 from: 1 to: 1386
KIEE1591.orf
With 182 enzymes: SgfI *
July 15, 2010 18:42 ..
AccI GT'mk_AC
Cuts at: 0 1082 1386
Size: 1082 304
Acc65I G'GTAC_C
Cuts at: 0 53 1386
Size: 53 1333
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 224 1213 1386
Size: 224 989 173
Fragments arranged by size:
989 224 173
AflIII A'CryG_T
Cuts at: 0 365 367 648 1115 1386
Size: 365 2 281 467 271
Fragments arranged by size:
467 365 281 271 2
AlwNI CAG_nnn'CTG
Cuts at: 0 20 311 948 1023 1386
Size: 20 291 637 75 363
Fragments arranged by size:
637 363 291 75 20
AvaI C'yCGr_G
Cuts at: 0 514 835 1206 1386
Size: 514 321 371 180
Fragments arranged by size:
514 371 321 180
BanI G'GyrC_C
Cuts at: 0 41 53 60 504 1386
Size: 41 12 7 444 882
Fragments arranged by size:
882 444 41 12 7
BanII G_rGCy'C
Cuts at: 0 560 1213 1386
Size: 560 653 173
Fragments arranged by size:
653 560 173
BbvCI CC'TCA_GC
Cuts at: 0 1342 1386
Size: 1342 44
BclI T'GATC_A
Cuts at: 0 1184 1386
Size: 1184 202
BglI GCCn_nnn'nGGC
Cuts at: 0 614 1171 1386
Size: 614 557 215
Fragments arranged by size:
614 557 215
BlpI GC'TnA_GC
Cuts at: 0 985 1175 1386
Size: 985 190 211
Fragments arranged by size:
985 211 190
Bme1580I G_kGCm'C
Cuts at: 0 44 218 509 1386
Size: 44 174 291 877
Fragments arranged by size:
877 291 174 44
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 115 976 1045 1087 1386
Size: 115 861 69 42 299
Fragments arranged by size:
861 299 115 69 42
Bpu10I CC'TnA_GC
Cuts at: 0 1342 1386
Size: 1342 44
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 766 1386
Size: 766 620
BsaI GGTCTCn'nnnn_
Cuts at: 0 178 1004 1386
Size: 178 826 382
Fragments arranged by size:
826 382 178
BsaAI yAC'GTr
Cuts at: 0 368 1086 1386
Size: 368 718 300
Fragments arranged by size:
718 368 300
BsaBI GATnn'nnATC
Cuts at: 0 1201 1386
Size: 1201 185
BsaHI Gr'CG_yC
Cuts at: 0 541 1386
Size: 541 845
BsaWI w'CCGG_w
Cuts at: 0 878 1386
Size: 878 508
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1 31 1386
Size: 1 30 1355
Fragments arranged by size:
1355 30 1
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 769 1386
Size: 769 617
BseYI C'CCAG_C
Cuts at: 0 93 171 217 612 1102 1336 1348 1386
Size: 93 78 46 395 490 234 12 38
Fragments arranged by size:
490 395 234 93 78 46 38 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 804 1386
Size: 804 582
BsiEI CG_ry'CG
Cuts at: 0 483 1386
Size: 483 903
BsiHKAI G_wGCw'C
Cuts at: 0 523 560 1386
Size: 523 37 826
Fragments arranged by size:
826 523 37
BsmBI CGTCTCn'nnnn_
Cuts at: 0 967 1386
Size: 967 419
Bsp1286I G_dGCh'C
Cuts at: 0 44 218 509 523 560 1213 1386
Size: 44 174 291 14 37 653 173
Fragments arranged by size:
653 291 174 173 44 37 14
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1157 1386
Size: 1157 229
BsrBI CCG'CTC
Cuts at: 0 1179 1271 1386
Size: 1179 92 115
Fragments arranged by size:
1179 115 92
BsrGI T'GTAC_A
Cuts at: 0 362 1386
Size: 362 1024
BstAPI GCAn_nnn'nTGC
Cuts at: 0 20 1386
Size: 20 1366
BstEII G'GTnAC_C
Cuts at: 0 402 1386
Size: 402 984
BstYI r'GATC_y
Cuts at: 0 815 1202 1386
Size: 815 387 184
Fragments arranged by size:
815 387 184
Bsu36I CC'TnA_GG
Cuts at: 0 285 1386
Size: 285 1101
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 744 1386
Size: 744 642
DraIII CAC_nnn'GTG
Cuts at: 0 701 1386
Size: 701 685
EaeI y'GGCC_r
Cuts at: 0 2 1370 1386
Size: 2 1368 16
Fragments arranged by size:
1368 16 2
EarI CTCTTCn'nnn_
Cuts at: 0 200 1386
Size: 200 1186
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 325 1386
Size: 325 1061
EcoICRI GAG'CTC
Cuts at: 0 558 1386
Size: 558 828
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 115 224 976 1045 1087 1213 1386
Size: 115 109 752 69 42 126 173
Fragments arranged by size:
752 173 126 115 109 69 42
EcoNI CCTnn'n_nnAGG
Cuts at: 0 781 1386
Size: 781 605
EcoO109I rG'GnC_Cy
Cuts at: 0 1020 1254 1386
Size: 1020 234 132
Fragments arranged by size:
1020 234 132
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 279 348 1386
Size: 279 69 1038
Fragments arranged by size:
1038 279 69
FspI TGC'GCA
Cuts at: 0 574 1386
Size: 574 812
HaeII r_GCGC'y
Cuts at: 0 119 311 992 1075 1166 1386
Size: 119 192 681 83 91 220
Fragments arranged by size:
681 220 192 119 91 83
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 268 300 1386
Size: 268 32 1086
Fragments arranged by size:
1086 268 32
KpnI G_GTAC'C
Cuts at: 0 57 1386
Size: 57 1329
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1220 1379 1386
Size: 1220 159 7
Fragments arranged by size:
1220 159 7
MscI TGG'CCA
Cuts at: 0 4 1386
Size: 4 1382
MslI CAynn'nnrTG
Cuts at: 0 47 1386
Size: 47 1339
MspA1I CmG'CkG
Cuts at: 0 416 612 735 945 1062 1328 1386
Size: 416 196 123 210 117 266 58
Fragments arranged by size:
416 266 210 196 123 117 58
NspI r_CATG'y
Cuts at: 0 459 1050 1119 1386
Size: 459 591 69 267
Fragments arranged by size:
591 459 267 69
PciI A'CATG_T
Cuts at: 0 1115 1386
Size: 1115 271
PflMI CCAn_nnn'nTGG
Cuts at: 0 151 1023 1386
Size: 151 872 363
Fragments arranged by size:
872 363 151
PfoI T'CCnGG_A
Cuts at: 0 1229 1386
Size: 1229 157
PmlI CAC'GTG
Cuts at: 0 368 1386
Size: 368 1018
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 183 215 622 654 1386
Size: 183 32 407 32 732
Fragments arranged by size:
732 407 183 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 1020 1386
Size: 1020 366
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 346 378 1386
Size: 346 32 1008
Fragments arranged by size:
1008 346 32
PstI C_TGCA'G
Cuts at: 0 437 1055 1386
Size: 437 618 331
Fragments arranged by size:
618 437 331
PvuII CAG'CTG
Cuts at: 0 612 735 945 1328 1386
Size: 612 123 210 383 58
Fragments arranged by size:
612 383 210 123 58
RsrII CG'GwC_CG
Cuts at: 0 1035 1386
Size: 1035 351
SacI G_AGCT'C
Cuts at: 0 560 1386
Size: 560 826
ScaI AGT'ACT
Cuts at: 0 424 1386
Size: 424 962
SexAI A'CCwGG_T
Cuts at: 0 920 1386
Size: 920 466
SfcI C'TryA_G
Cuts at: 0 433 1051 1329 1386
Size: 433 618 278 57
Fragments arranged by size:
618 433 278 57
SmaI CCC'GGG
Cuts at: 0 1208 1386
Size: 1208 178
SmlI C'TyrA_G
Cuts at: 0 781 1386
Size: 781 605
StyI C'CwwG_G
Cuts at: 0 461 1166 1386
Size: 461 705 220
Fragments arranged by size:
705 461 220
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 85 497 1386
Size: 85 412 889
Fragments arranged by size:
889 412 85
TatI w'GTAC_w
Cuts at: 0 362 422 1386
Size: 362 60 964
Fragments arranged by size:
964 362 60
Tth111I GACn'n_nGTC
Cuts at: 0 922 1386
Size: 922 464
XmaI C'CCGG_G
Cuts at: 0 1206 1386
Size: 1206 180
Enzymes that do cut:
AccI Acc65I AcuI AflIII AlwNI AvaI BanI BanII
BbvCI BclI BglI BlpI Bme1580I BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrGI
BstAPI BstEII BstYI Bsu36I BtgZI DraIII EaeI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I FalI FspI HaeII
Hin4I KpnI MmeI MscI MslI MspA1I NspI PciI
PflMI PfoI PmlI PpiI PpuMI PsrI PstI PvuII
RsrII SacI ScaI SexAI SfcI SmaI SmlI StyI
TaqII TatI Tth111I XmaI
Enzymes that do not cut:
AarI AatII AclI AfeI AflII AgeI AhdI AleI
AlfI AloI ApaI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BbeI BbsI BcgI BciVI BfrBI
BglII BmgBI BmrI BmtI BplI BsiWI BsmI BspEI
BspHI BsrDI BsrFI BssHII BssSI BstBI BstXI BstZ17I
BtgI BtsI ClaI CspCI DraI DrdI EagI EcoRI
EcoRV FseI FspAI HincII HindIII HpaI KasI MfeI
MluI NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI NsiI PacI PasI PmeI PshAI PsiI PspOMI
PspXI PvuI SacII SalI SanDI SapI SbfI SfiI
SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SspI
StuI SwaI TstI XbaI XcmI XhoI XmnI ZraI