(Linear) (Six-base) MAPSORT of: KIEE1586.seq  Check: 5623  from: 1  to: 282



KIEE1586.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:42  ..

AflIII A'CryG_T

Cuts at:      0      70     282 
   Size:         70     212
 
ApoI r'AATT_y

Cuts at:      0     151     282 
   Size:        151     131
 
BsaAI yAC'GTr

Cuts at:      0      73     282 
   Size:         73     209
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     154     184     282 
   Size:        154      30      98

  Fragments arranged by size: 

                 154      98      30
 
BseYI C'CCAG_C

Cuts at:      0     173     282 
   Size:        173     109
 
BsiHKAI G_wGCw'C

Cuts at:      0      32     282 
   Size:         32     250
 
Bsp1286I G_dGCh'C

Cuts at:      0      32     282 
   Size:         32     250
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     103     282 
   Size:        103     179
 
EcoRI G'AATT_C

Cuts at:      0     151     282 
   Size:        151     131
 
MfeI C'AATT_G

Cuts at:      0     277     282 
   Size:        277       5
 
MspA1I CmG'CkG

Cuts at:      0     167     177     282 
   Size:        167      10     105

  Fragments arranged by size: 

                 167     105      10
 
PmlI CAC'GTG

Cuts at:      0      73     282 
   Size:         73     209
 
PvuII CAG'CTG

Cuts at:      0     167     282 
   Size:        167     115
 
StyI C'CwwG_G

Cuts at:      0      14      57     226     282 
   Size:         14      43     169      56

  Fragments arranged by size: 

                 169      56      43      14
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     126     228     282 
   Size:        126     102      54

  Fragments arranged by size: 

                 126     102      54
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     233     282 
   Size:        233      49
 

 Enzymes that do cut:

   AflIII     ApoI    BsaAI    BsaXI    BseYI  BsiHKAI Bsp1286I    BspMI
    EcoRI     MfeI   MspA1I     PmlI    PvuII     StyI    TaqII     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI
     BpmI   Bpu10I    BpuEI     BsaI    BsaBI    BsaHI    BsaWI    BseRI
     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrBI
    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI
    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EarI     EciI
  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRV     FalI     FseI     FspI
    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI     KasI     KpnI
     MluI     MmeI     MscI     MslI     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI    PflMI     PfoI     PmeI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     SwaI     TatI     TstI  Tth111I     XbaI
     XhoI     XmaI     XmnI     ZraI