(Linear) (Six-base) MAPSORT of: KIEE1584.seq  Check: 1207  from: 1  to: 276



KIEE1584.orf

 With 182 enzymes: SgfI * 

                        July 3, 2012 16:56  ..

AfeI AGC'GCT

Cuts at:      0      62     276 
   Size:         62     214
 
AflIII A'CryG_T

Cuts at:      0     254     276 
   Size:        254      22
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     236     276 
   Size:        236      40
 
AlwNI CAG_nnn'CTG

Cuts at:      0     170     276 
   Size:        170     106
 
BanII G_rGCy'C

Cuts at:      0      82     276 
   Size:         82     194
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     224     276 
   Size:        224      52
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      41     276 
   Size:         41     235
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     151     229     276 
   Size:        151      78      47

  Fragments arranged by size: 

                 151      78      47
 
BsaAI yAC'GTr

Cuts at:      0     255     276 
   Size:        255      21
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     241     271     276 
   Size:        241      30       5

  Fragments arranged by size: 

                 241      30       5
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      19     276 
   Size:         19     257
 
BseYI C'CCAG_C

Cuts at:      0     176     276 
   Size:        176     100
 
Bsp1286I G_dGCh'C

Cuts at:      0      82     276 
   Size:         82     194
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     170     276 
   Size:        170     106
 
DrdI GACnn_nn'nnGTC

Cuts at:      0      21     276 
   Size:         21     255
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      41     276 
   Size:         41     235
 
HaeII r_GCGC'y

Cuts at:      0      64     276 
   Size:         64     212
 
HindIII A'AGCT_T

Cuts at:      0     124     276 
   Size:        124     152
 
MspA1I CmG'CkG

Cuts at:      0     184     276 
   Size:        184      92
 
PfoI T'CCnGG_A

Cuts at:      0     245     276 
   Size:        245      31
 
PshAI GACnn'nnGTC

Cuts at:      0     241     276 
   Size:        241      35
 
PvuII CAG'CTG

Cuts at:      0     184     276 
   Size:        184      92
 

 Enzymes that do cut:

     AfeI   AflIII     AlfI    AlwNI    BanII     BmrI     BpmI     BsaI
    BsaAI    BsaXI    BseRI    BseYI Bsp1286I   BstAPI     DrdI  Eco57MI
    HaeII  HindIII   MspA1I     PfoI    PshAI    PvuII

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI    AflII     AgeI
     AhdI     AleI     AloI     ApaI    ApaLI     ApoI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI     BbeI     BbsI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmtI     BplI   Bpu10I    BpuEI    BsaBI    BsaHI
    BsaWI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI
     BtsI     ClaI    CspCI     DraI   DraIII     EaeI     EagI     EarI
     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI    Hin4I   HincII     HpaI     KasI     KpnI     MfeI
     MluI     MmeI     MscI     MslI     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI    PflMI     PmeI     PmlI     PpiI    PpuMI     PsiI   PspOMI
    PspXI     PsrI     PstI     PvuI    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     StyI     SwaI    TaqII     TatI     TstI  Tth111I
     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI