(Linear) (Six-base) MAPSORT of: KIEE1581.seq  Check: 4694  from: 1  to: 267



KIEE1581.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:42  ..

AflIII A'CryG_T

Cuts at:      0      35     267 
   Size:         35     232
 
AlwNI CAG_nnn'CTG

Cuts at:      0      84     267 
   Size:         84     183
 
ApoI r'AATT_y

Cuts at:      0     133     267 
   Size:        133     134
 
BclI T'GATC_A

Cuts at:      0      20     267 
   Size:         20     247
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     106     267 
   Size:        106     161
 
BsiEI CG_ry'CG

Cuts at:      0       9     267 
   Size:          9     258
 
BsrDI GCAATG_nn'

Cuts at:      0     110     162     267 
   Size:        110      52     105

  Fragments arranged by size: 

                 110     105      52
 
EaeI y'GGCC_r

Cuts at:      0     242     267 
   Size:        242      25
 
EarI CTCTTCn'nnn_

Cuts at:      0      38     267 
   Size:         38     229
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     106     267 
   Size:        106     161
 
HaeII r_GCGC'y

Cuts at:      0      85     267 
   Size:         85     182
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      20     267 
   Size:         20     247
 
MscI TGG'CCA

Cuts at:      0     244     267 
   Size:        244      23
 
NspI r_CATG'y

Cuts at:      0      39     267 
   Size:         39     228
 
PciI A'CATG_T

Cuts at:      0      35     267 
   Size:         35     232
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      23     267 
   Size:         23     244
 
TatI w'GTAC_w

Cuts at:      0     146     267 
   Size:        146     121
 

 Enzymes that do cut:

   AflIII    AlwNI     ApoI     BclI     BpmI    BsiEI    BsrDI     EaeI
     EarI  Eco57MI    HaeII     MmeI     MscI     NspI     PciI    TaqII
     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI     BbsI    BbvCI     BcgI    BciVI    BfrBI     BglI    BglII
     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
     BsgI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI
   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI
     ClaI    CspCI     DraI   DraIII     DrdI     EagI     EciI  EcoICRI
    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MslI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     PacI     PasI    PflMI     PfoI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     StyI     SwaI     TstI  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI