(Linear) (Six-base) MAPSORT of: KIEE1580.seq  Check: 6512  from: 1  to: 255



KIEE1580.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:42  ..

ApoI r'AATT_y

Cuts at:      0     250     255 
   Size:        250       5
 
BclI T'GATC_A

Cuts at:      0     179     255 
   Size:        179      76
 
BlpI GC'TnA_GC

Cuts at:      0     148     255 
   Size:        148     107
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      97     255 
   Size:         97     158
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      73     255 
   Size:         73     182
 
BstBI TT'CG_AA

Cuts at:      0      66     255 
   Size:         66     189
 
BtsI GCAGTG_nn'

Cuts at:      0     129     159     255 
   Size:        129      30      96

  Fragments arranged by size: 

                 129      96      30
 
EarI CTCTTCn'nnn_

Cuts at:      0      44     255 
   Size:         44     211
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      97     255 
   Size:         97     158
 
EcoO109I rG'GnC_Cy

Cuts at:      0      72     255 
   Size:         72     183
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     171     203     255 
   Size:        171      32      52

  Fragments arranged by size: 

                 171      52      32
 
HindIII A'AGCT_T

Cuts at:      0      34     255 
   Size:         34     221
 
PpuMI rG'GwC_Cy

Cuts at:      0      72     255 
   Size:         72     183
 
PsiI TTA'TAA

Cuts at:      0     200     255 
   Size:        200      55
 

 Enzymes that do cut:

     ApoI     BclI     BlpI     BpmI     BsaI    BstBI     BtsI     EarI
  Eco57MI EcoO109I    Hin4I  HindIII    PpuMI     PsiI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
     BanI    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI    BfrBI
     BglI    BglII Bme1580I    BmgBI     BmrI     BmtI     BplI   Bpu10I
    BpuEI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI
     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EciI
  EcoICRI    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
    HaeII   HincII     HpaI     KasI     KpnI     MfeI     MluI     MmeI
     MscI     MslI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PshAI   PspOMI    PspXI
     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     StyI     SwaI    TaqII     TatI     TstI  Tth111I
     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI