Restriction map for
pFN21AE1576
(Linear) (Six-base) MAPSORT of: KIEE1576.seq Check: 4404 from: 1 to: 435
KIEE1576.orf
With 182 enzymes: SgfI *
July 15, 2010 18:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 127 435
Size: 127 308
AccI GT'mk_AC
Cuts at: 0 35 161 435
Size: 35 126 274
Fragments arranged by size:
274 126 35
Acc65I G'GTAC_C
Cuts at: 0 167 435
Size: 167 268
AflIII A'CryG_T
Cuts at: 0 424 426 435
Size: 424 2 9
Fragments arranged by size:
424 9 2
ApaI G_GGCC'C
Cuts at: 0 241 357 435
Size: 241 116 78
Fragments arranged by size:
241 116 78
BamHI G'GATC_C
Cuts at: 0 265 435
Size: 265 170
BanI G'GyrC_C
Cuts at: 0 167 435
Size: 167 268
BanII G_rGCy'C
Cuts at: 0 12 241 357 435
Size: 12 229 116 78
Fragments arranged by size:
229 116 78 12
BbvCI CC'TCA_GC
Cuts at: 0 56 435
Size: 56 379
Bme1580I G_kGCm'C
Cuts at: 0 241 357 435
Size: 241 116 78
Fragments arranged by size:
241 116 78
Bpu10I CC'TnA_GC
Cuts at: 0 56 435
Size: 56 379
BsaAI yAC'GTr
Cuts at: 0 427 435
Size: 427 8
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 373 403 435
Size: 373 30 32
Fragments arranged by size:
373 32 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 388 435
Size: 388 47
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 24 105 435
Size: 24 81 330
Fragments arranged by size:
330 81 24
Bsp1286I G_dGCh'C
Cuts at: 0 12 241 357 435
Size: 12 229 116 78
Fragments arranged by size:
229 116 78 12
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 127 435
Size: 127 308
BsrFI r'CCGG_y
Cuts at: 0 60 78 435
Size: 60 18 357
Fragments arranged by size:
357 60 18
BsrGI T'GTAC_A
Cuts at: 0 152 435
Size: 152 283
BstXI CCAn_nnnn'nTGG
Cuts at: 0 276 435
Size: 276 159
BstYI r'GATC_y
Cuts at: 0 265 435
Size: 265 170
Bsu36I CC'TnA_GG
Cuts at: 0 127 320 435
Size: 127 193 115
Fragments arranged by size:
193 127 115
BtsI GCAGTG_nn'
Cuts at: 0 410 435
Size: 410 25
EaeI y'GGCC_r
Cuts at: 0 80 278 435
Size: 80 198 157
Fragments arranged by size:
198 157 80
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 100 435
Size: 100 335
EcoO109I rG'GnC_Cy
Cuts at: 0 237 238 246 353 435
Size: 237 1 8 107 82
Fragments arranged by size:
237 107 82 8 1
KpnI G_GTAC'C
Cuts at: 0 171 435
Size: 171 264
MscI TGG'CCA
Cuts at: 0 280 435
Size: 280 155
MslI CAynn'nnrTG
Cuts at: 0 148 435
Size: 148 287
NaeI GCC'GGC
Cuts at: 0 80 435
Size: 80 355
NgoMIV G'CCGG_C
Cuts at: 0 78 435
Size: 78 357
PmlI CAC'GTG
Cuts at: 0 427 435
Size: 427 8
PpuMI rG'GwC_Cy
Cuts at: 0 246 435
Size: 246 189
PshAI GACnn'nnGTC
Cuts at: 0 427 435
Size: 427 8
PspOMI G'GGCC_C
Cuts at: 0 237 353 435
Size: 237 116 82
Fragments arranged by size:
237 116 82
PstI C_TGCA'G
Cuts at: 0 317 417 435
Size: 317 100 18
Fragments arranged by size:
317 100 18
SfcI C'TryA_G
Cuts at: 0 313 413 435
Size: 313 100 22
Fragments arranged by size:
313 100 22
StyI C'CwwG_G
Cuts at: 0 96 435
Size: 96 339
TatI w'GTAC_w
Cuts at: 0 152 435
Size: 152 283
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 373 405 435
Size: 373 32 30
Fragments arranged by size:
373 32 30
Enzymes that do cut:
AarI AccI Acc65I AflIII ApaI BamHI BanI BanII
BbvCI Bme1580I Bpu10I BsaAI BsaXI BseRI BsgI Bsp1286I
BspMI BsrFI BsrGI BstXI BstYI Bsu36I BtsI EaeI
EciI EcoO109I KpnI MscI MslI NaeI NgoMIV PmlI
PpuMI PshAI PspOMI PstI SfcI StyI TatI TstI
Enzymes that do not cut:
AatII AclI AcuI AfeI AflII AgeI AhdI AleI
AlfI AloI AlwNI ApaLI ApoI AscI AseI AsiSI
AvaI AvrII BaeI BbeI BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI BmgBI BmrI BmtI BplI
BpmI BpuEI BsaI BsaBI BsaHI BsaWI BseYI BsiEI
BsiHKAI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrDI
BssHII BssSI BstAPI BstBI BstEII BstZ17I BtgI BtgZI
ClaI CspCI DraI DraIII DrdI EagI EarI EcoICRI
Eco57MI EcoNI EcoRI EcoRV FalI FseI FspI FspAI
HaeII Hin4I HincII HindIII HpaI KasI MfeI MluI
MmeI MspA1I NarI NcoI NdeI NheI NotI NruI
NsiI NspI PacI PasI PciI PflMI PfoI PmeI
PpiI PsiI PspXI PsrI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StuI SwaI TaqII Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI