Restriction map for
pFN21AE1575
(Linear) (Six-base) MAPSORT of: KIEE1575.seq Check: 8570 from: 1 to: 1095
KIEE1575.orf
With 182 enzymes: SgfI *
July 15, 2010 18:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 853 1095
Size: 853 242
Acc65I G'GTAC_C
Cuts at: 0 407 1095
Size: 407 688
AleI CACnn'nnGTG
Cuts at: 0 103 1095
Size: 103 992
ApaI G_GGCC'C
Cuts at: 0 48 687 1095
Size: 48 639 408
Fragments arranged by size:
639 408 48
AvaI C'yCGr_G
Cuts at: 0 39 1095
Size: 39 1056
BanI G'GyrC_C
Cuts at: 0 12 216 407 816 1095
Size: 12 204 191 409 279
Fragments arranged by size:
409 279 204 191 12
BanII G_rGCy'C
Cuts at: 0 48 76 131 687 761 1009 1066 1095
Size: 48 28 55 556 74 248 57 29
Fragments arranged by size:
556 248 74 57 55 48 29 28
BbeI G_GCGC'C
Cuts at: 0 16 220 1095
Size: 16 204 875
Fragments arranged by size:
875 204 16
BbsI GAAGACnn'nnnn_
Cuts at: 0 893 1095
Size: 893 202
BglI GCCn_nnn'nGGC
Cuts at: 0 10 390 526 1095
Size: 10 380 136 569
Fragments arranged by size:
569 380 136 10
Bme1580I G_kGCm'C
Cuts at: 0 48 687 1059 1095
Size: 48 639 372 36
Fragments arranged by size:
639 372 48 36
BmgBI CAC'GTC
Cuts at: 0 378 1095
Size: 378 717
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1049 1095
Size: 1049 46
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 571 607 989 1095
Size: 571 36 382 106
Fragments arranged by size:
571 382 106 36
Bpu10I CC'TnA_GC
Cuts at: 0 688 1095
Size: 688 407
BsaI GGTCTCn'nnnn_
Cuts at: 0 760 1095
Size: 760 335
BsaAI yAC'GTr
Cuts at: 0 153 1095
Size: 153 942
BsaHI Gr'CG_yC
Cuts at: 0 13 188 217 620 805 1095
Size: 13 175 29 403 185 290
Fragments arranged by size:
403 290 185 175 29 13
BsaWI w'CCGG_w
Cuts at: 0 254 1095
Size: 254 841
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 544 574 962 992 1095
Size: 544 30 388 30 103
Fragments arranged by size:
544 388 103 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1011 1095
Size: 1011 84
BseYI C'CCAG_C
Cuts at: 0 500 680 905 1095
Size: 500 180 225 190
Fragments arranged by size:
500 225 190 180
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 874 1095
Size: 874 221
BsiEI CG_ry'CG
Cuts at: 0 120 1095
Size: 120 975
BsiHKAI G_wGCw'C
Cuts at: 0 761 1009 1095
Size: 761 248 86
Fragments arranged by size:
761 248 86
BsmBI CGTCTCn'nnnn_
Cuts at: 0 508 595 1095
Size: 508 87 500
Fragments arranged by size:
508 500 87
Bsp1286I G_dGCh'C
Cuts at: 0 48 76 131 687 761 1009 1059 1066
Size: 48 28 55 556 74 248 50 7
Cuts at: 1066 1095
Size: 29
Fragments arranged by size:
556 248 74 55 50 48 29 28
7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 467 853 1095
Size: 467 386 242
Fragments arranged by size:
467 386 242
BsrDI GCAATG_nn'
Cuts at: 0 932 1095
Size: 932 163
BsrFI r'CCGG_y
Cuts at: 0 557 1095
Size: 557 538
BssSI C'ACGA_G
Cuts at: 0 760 1095
Size: 760 335
BstAPI GCAn_nnn'nTGC
Cuts at: 0 171 1095
Size: 171 924
Bsu36I CC'TnA_GG
Cuts at: 0 673 1095
Size: 673 422
BtgI C'CryG_G
Cuts at: 0 120 143 211 220 402 473 1095
Size: 120 23 68 9 182 71 622
Fragments arranged by size:
622 182 120 71 68 23 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 123 426 1095
Size: 123 303 669
Fragments arranged by size:
669 303 123
BtsI GCAGTG_nn'
Cuts at: 0 541 1053 1095
Size: 541 512 42
Fragments arranged by size:
541 512 42
EaeI y'GGCC_r
Cuts at: 0 2 117 1095
Size: 2 115 978
Fragments arranged by size:
978 115 2
EagI C'GGCC_G
Cuts at: 0 117 1095
Size: 117 978
EarI CTCTTCn'nnn_
Cuts at: 0 997 1095
Size: 997 98
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 493 544 1095
Size: 493 51 551
Fragments arranged by size:
551 493 51
EcoICRI GAG'CTC
Cuts at: 0 759 1007 1095
Size: 759 248 88
Fragments arranged by size:
759 248 88
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 571 607 989 1095
Size: 571 36 382 106
Fragments arranged by size:
571 382 106 36
EcoNI CCTnn'n_nnAGG
Cuts at: 0 451 1095
Size: 451 644
EcoO109I rG'GnC_Cy
Cuts at: 0 44 45 456 684 788 1095
Size: 44 1 411 228 104 307
Fragments arranged by size:
411 307 228 104 44 1
HaeII r_GCGC'y
Cuts at: 0 16 220 396 1095
Size: 16 204 176 699
Fragments arranged by size:
699 204 176 16
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 448 480 947 962 979 994 1095
Size: 448 32 467 15 17 15 101
Fragments arranged by size:
467 448 101 32 17 15 15
KasI G'GCGC_C
Cuts at: 0 12 216 1095
Size: 12 204 879
Fragments arranged by size:
879 204 12
KpnI G_GTAC'C
Cuts at: 0 411 1095
Size: 411 684
MslI CAynn'nnrTG
Cuts at: 0 103 142 1095
Size: 103 39 953
Fragments arranged by size:
953 103 39
MspA1I CmG'CkG
Cuts at: 0 122 213 404 475 726 1095
Size: 122 91 191 71 251 369
Fragments arranged by size:
369 251 191 122 91 71
NarI GG'CG_CC
Cuts at: 0 13 217 1095
Size: 13 204 878
Fragments arranged by size:
878 204 13
NdeI CA'TA_TG
Cuts at: 0 635 1095
Size: 635 460
PasI CC'CwG_GG
Cuts at: 0 1059 1095
Size: 1059 36
PflMI CCAn_nnn'nTGG
Cuts at: 0 204 1095
Size: 204 891
PpuMI rG'GwC_Cy
Cuts at: 0 456 788 1095
Size: 456 332 307
Fragments arranged by size:
456 332 307
PspOMI G'GGCC_C
Cuts at: 0 44 683 1095
Size: 44 639 412
Fragments arranged by size:
639 412 44
PstI C_TGCA'G
Cuts at: 0 611 780 1095
Size: 611 169 315
Fragments arranged by size:
611 315 169
SacI G_AGCT'C
Cuts at: 0 761 1009 1095
Size: 761 248 86
Fragments arranged by size:
761 248 86
SacII CC_GC'GG
Cuts at: 0 123 214 405 476 1095
Size: 123 91 191 71 619
Fragments arranged by size:
619 191 123 91 71
SapI GCTCTTCn'nnn_
Cuts at: 0 997 1095
Size: 997 98
SbfI CC_TGCA'GG
Cuts at: 0 780 1095
Size: 780 315
SfcI C'TryA_G
Cuts at: 0 607 776 1095
Size: 607 169 319
Fragments arranged by size:
607 319 169
SfoI GGC'GCC
Cuts at: 0 14 218 1095
Size: 14 204 877
Fragments arranged by size:
877 204 14
StuI AGG'CCT
Cuts at: 0 774 1095
Size: 774 321
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 308 514 1095
Size: 308 206 581
Fragments arranged by size:
581 308 206
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 544 576 1095
Size: 544 32 519
Fragments arranged by size:
544 519 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 364 921 1095
Size: 364 557 174
Fragments arranged by size:
557 364 174
Enzymes that do cut:
AarI Acc65I AleI ApaI AvaI BanI BanII BbeI
BbsI BglI Bme1580I BmgBI BplI BpmI Bpu10I BsaI
BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspMI BsrDI BsrFI BssSI BstAPI
Bsu36I BtgI BtgZI BtsI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I KasI KpnI
MslI MspA1I NarI NdeI PasI PflMI PpuMI PspOMI
PstI SacI SacII SapI SbfI SfcI SfoI StuI
TaqII TstI XcmI
Enzymes that do not cut:
AatII AccI AclI AcuI AfeI AflII AflIII AgeI
AhdI AlfI AloI AlwNI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BbvCI BcgI BciVI BclI
BfrBI BglII BlpI BmrI BmtI BpuEI BsaBI BsiWI
BsmI BspEI BspHI BsrBI BsrGI BssHII BstBI BstEII
BstXI BstYI BstZ17I ClaI CspCI DraI DraIII DrdI
EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII
HpaI MfeI MluI MmeI MscI NaeI NcoI NgoMIV
NheI NotI NruI NsiI NspI PacI PciI PfoI
PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI
PvuII RsrII SalI SanDI ScaI SexAI SfiI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StyI SwaI TatI Tth111I XbaI XhoI XmaI XmnI
ZraI