Restriction map for
pFN21AE1574
(Linear) (Six-base) MAPSORT of: KIEE1574.seq Check: 5739 from: 1 to: 2049
KIEE1574.orf
With 182 enzymes: SgfI *
July 15, 2010 18:42 ..
AccI GT'mk_AC
Cuts at: 0 1607 2049
Size: 1607 442
Acc65I G'GTAC_C
Cuts at: 0 203 2049
Size: 203 1846
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 479 974 1546 2049
Size: 479 495 572 503
Fragments arranged by size:
572 503 495 479
AflIII A'CryG_T
Cuts at: 0 140 2049
Size: 140 1909
AleI CACnn'nnGTG
Cuts at: 0 1718 2049
Size: 1718 331
AlwNI CAG_nnn'CTG
Cuts at: 0 1156 1362 1945 2049
Size: 1156 206 583 104
Fragments arranged by size:
1156 583 206 104
ApaI G_GGCC'C
Cuts at: 0 52 404 1755 2049
Size: 52 352 1351 294
Fragments arranged by size:
1351 352 294 52
ApaLI G'TGCA_C
Cuts at: 0 685 2049
Size: 685 1364
ApoI r'AATT_y
Cuts at: 0 588 1248 1296 2049
Size: 588 660 48 753
Fragments arranged by size:
753 660 588 48
BamHI G'GATC_C
Cuts at: 0 340 2049
Size: 340 1709
BanI G'GyrC_C
Cuts at: 0 203 2049
Size: 203 1846
BanII G_rGCy'C
Cuts at: 0 52 404 794 1647 1755 2049
Size: 52 352 390 853 108 294
Fragments arranged by size:
853 390 352 294 108 52
BbsI GAAGACnn'nnnn_
Cuts at: 0 1608 2049
Size: 1608 441
BbvCI CC'TCA_GC
Cuts at: 0 382 2049
Size: 382 1667
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 649 683 2049
Size: 649 34 1366
Fragments arranged by size:
1366 649 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 895 2049
Size: 895 1154
BglI GCCn_nnn'nGGC
Cuts at: 0 131 2049
Size: 131 1918
BglII A'GATC_T
Cuts at: 0 1955 2049
Size: 1955 94
Bme1580I G_kGCm'C
Cuts at: 0 52 404 645 689 997 1755 2049
Size: 52 352 241 44 308 758 294
Fragments arranged by size:
758 352 308 294 241 52 44
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1009 1024 1886 2049
Size: 1009 15 862 163
Fragments arranged by size:
1009 862 163 15
Bpu10I CC'TnA_GC
Cuts at: 0 382 2049
Size: 382 1667
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1972 2049
Size: 1972 77
BsaI GGTCTCn'nnnn_
Cuts at: 0 316 754 905 976 1579 2049
Size: 316 438 151 71 603 470
Fragments arranged by size:
603 470 438 316 151 71
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 172 2049
Size: 172 1877
BsiHKAI G_wGCw'C
Cuts at: 0 689 2049
Size: 689 1360
Bsp1286I G_dGCh'C
Cuts at: 0 52 404 645 689 794 997 1647 1755
Size: 52 352 241 44 105 203 650 108
Cuts at: 1755 2049
Size: 294
Fragments arranged by size:
650 352 294 241 203 108 105 52
44
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 923 2049
Size: 923 1126
BsrBI CCG'CTC
Cuts at: 0 1224 2049
Size: 1224 825
BsrDI GCAATG_nn'
Cuts at: 0 489 2049
Size: 489 1560
BsrFI r'CCGG_y
Cuts at: 0 1192 2049
Size: 1192 857
BsrGI T'GTAC_A
Cuts at: 0 359 2049
Size: 359 1690
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1945 2049
Size: 1945 104
BstXI CCAn_nnnn'nTGG
Cuts at: 0 681 2049
Size: 681 1368
BstYI r'GATC_y
Cuts at: 0 340 602 860 1955 2049
Size: 340 262 258 1095 94
Fragments arranged by size:
1095 340 262 258 94
Bsu36I CC'TnA_GG
Cuts at: 0 1916 2049
Size: 1916 133
BtgI C'CryG_G
Cuts at: 0 1350 2049
Size: 1350 699
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1419 2049
Size: 1419 630
BtsI GCAGTG_nn'
Cuts at: 0 205 2049
Size: 205 1844
ClaI AT'CG_AT
Cuts at: 0 695 2049
Size: 695 1354
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1155 1190 1850 1885 2049
Size: 1155 35 660 35 164
Fragments arranged by size:
1155 660 164 35 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 475 2049
Size: 475 1574
EaeI y'GGCC_r
Cuts at: 0 816 1856 2049
Size: 816 1040 193
Fragments arranged by size:
1040 816 193
EarI CTCTTCn'nnn_
Cuts at: 0 13 2049
Size: 13 2036
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 479 974 1009 1024 1546 1886 2049
Size: 479 495 35 15 522 340 163
Fragments arranged by size:
522 495 479 340 163 35 15
EcoO109I rG'GnC_Cy
Cuts at: 0 49 70 270 400 1466 1751 1752 2049
Size: 49 21 200 130 1066 285 1 297
Fragments arranged by size:
1066 297 285 200 130 49 21 1
EcoRI G'AATT_C
Cuts at: 0 588 1248 2049
Size: 588 660 801
Fragments arranged by size:
801 660 588
HaeII r_GCGC'y
Cuts at: 0 7 2049
Size: 7 2042
HincII GTy'rAC
Cuts at: 0 489 1229 1824 2049
Size: 489 740 595 225
Fragments arranged by size:
740 595 489 225
KpnI G_GTAC'C
Cuts at: 0 207 2049
Size: 207 1842
MfeI C'AATT_G
Cuts at: 0 1277 2049
Size: 1277 772
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 318 617 2049
Size: 318 299 1432
Fragments arranged by size:
1432 318 299
MscI TGG'CCA
Cuts at: 0 1858 2049
Size: 1858 191
MslI CAynn'nnrTG
Cuts at: 0 577 1718 2049
Size: 577 1141 331
Fragments arranged by size:
1141 577 331
MspA1I CmG'CkG
Cuts at: 0 93 357 457 1019 1329 1430 2049
Size: 93 264 100 562 310 101 619
Fragments arranged by size:
619 562 310 264 101 100 93
NaeI GCC'GGC
Cuts at: 0 1194 2049
Size: 1194 855
NcoI C'CATG_G
Cuts at: 0 1350 2049
Size: 1350 699
NdeI CA'TA_TG
Cuts at: 0 521 2049
Size: 521 1528
NgoMIV G'CCGG_C
Cuts at: 0 1192 2049
Size: 1192 857
NspI r_CATG'y
Cuts at: 0 1029 1551 2049
Size: 1029 522 498
Fragments arranged by size:
1029 522 498
PasI CC'CwG_GG
Cuts at: 0 45 274 327 2049
Size: 45 229 53 1722
Fragments arranged by size:
1722 229 53 45
PflMI CCAn_nnn'nTGG
Cuts at: 0 525 2049
Size: 525 1524
PfoI T'CCnGG_A
Cuts at: 0 255 2049
Size: 255 1794
PpuMI rG'GwC_Cy
Cuts at: 0 70 270 1466 2049
Size: 70 200 1196 583
Fragments arranged by size:
1196 583 200 70
PspOMI G'GGCC_C
Cuts at: 0 48 400 1751 2049
Size: 48 352 1351 298
Fragments arranged by size:
1351 352 298 48
PstI C_TGCA'G
Cuts at: 0 104 1018 1152 1575 1898 2049
Size: 104 914 134 423 323 151
Fragments arranged by size:
914 423 323 151 134 104
PvuII CAG'CTG
Cuts at: 0 93 357 457 1329 1430 2049
Size: 93 264 100 872 101 619
Fragments arranged by size:
872 619 264 101 100 93
RsrII CG'GwC_CG
Cuts at: 0 366 2049
Size: 366 1683
SanDI GG'GwC_CC
Cuts at: 0 70 1466 2049
Size: 70 1396 583
Fragments arranged by size:
1396 583 70
SapI GCTCTTCn'nnn_
Cuts at: 0 13 2049
Size: 13 2036
ScaI AGT'ACT
Cuts at: 0 181 2049
Size: 181 1868
SfcI C'TryA_G
Cuts at: 0 100 1014 1148 1571 1894 2049
Size: 100 914 134 423 323 155
Fragments arranged by size:
914 423 323 155 134 100
SmlI C'TyrA_G
Cuts at: 0 1951 2049
Size: 1951 98
SphI G_CATG'C
Cuts at: 0 1551 2049
Size: 1551 498
StuI AGG'CCT
Cuts at: 0 387 439 850 2049
Size: 387 52 411 1199
Fragments arranged by size:
1199 411 387 52
StyI C'CwwG_G
Cuts at: 0 1350 1685 1776 2049
Size: 1350 335 91 273
Fragments arranged by size:
1350 335 273 91
TatI w'GTAC_w
Cuts at: 0 179 359 821 2049
Size: 179 180 462 1228
Fragments arranged by size:
1228 462 180 179
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 204 236 2049
Size: 204 32 1813
Fragments arranged by size:
1813 204 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 616 1866 2049
Size: 616 1250 183
Fragments arranged by size:
1250 616 183
XmnI GAAnn'nnTTC
Cuts at: 0 181 2049
Size: 181 1868
Enzymes that do cut:
AccI Acc65I AcuI AflIII AleI AlwNI ApaI ApaLI
ApoI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BglI BglII Bme1580I BpmI Bpu10I BpuEI BsaI BsgI
BsiHKAI Bsp1286I BspMI BsrBI BsrDI BsrFI BsrGI BstAPI
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DrdI EaeI EarI Eco57MI EcoO109I EcoRI HaeII HincII
KpnI MfeI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NspI PasI PflMI PfoI PpuMI PspOMI
PstI PvuII RsrII SanDI SapI ScaI SfcI SmlI
SphI StuI StyI TatI TstI XcmI XmnI
Enzymes that do not cut:
AarI AatII AclI AfeI AflII AgeI AhdI AlfI
AloI AscI AseI AsiSI AvaI AvrII BaeI BbeI
BclI BfrBI BlpI BmgBI BmrI BmtI BplI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiWI
BsmI BsmBI BspEI BspHI BssHII BssSI BstBI BstEII
BstZ17I DraI DraIII EagI EciI EcoICRI EcoNI EcoRV
FalI FseI FspI FspAI Hin4I HindIII HpaI KasI
MluI NarI NheI NotI NruI NsiI PacI PciI
PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI
SacI SacII SalI SbfI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SrfI SspI SwaI TaqII
Tth111I XbaI XhoI XmaI ZraI