Restriction map for
pFN21AE1570
(Linear) (Six-base) MAPSORT of: KIEE1570.seq Check: 7647 from: 1 to: 2028
KIEE1570.orf
With 182 enzymes: SgfI *
July 15, 2010 18:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1127 1263 1651 2028
Size: 1127 136 388 377
Fragments arranged by size:
1127 388 377 136
AccI GT'mk_AC
Cuts at: 0 1581 2028
Size: 1581 447
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 409 2028
Size: 409 1619
AflIII A'CryG_T
Cuts at: 0 1798 2028
Size: 1798 230
AhdI GACnn_n'nnGTC
Cuts at: 0 63 2028
Size: 63 1965
AleI CACnn'nnGTG
Cuts at: 0 1630 2028
Size: 1630 398
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 719 1741 2028
Size: 719 1022 287
Fragments arranged by size:
1022 719 287
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 244 276 2005 2028
Size: 244 32 1729 23
Fragments arranged by size:
1729 244 32 23
AlwNI CAG_nnn'CTG
Cuts at: 0 327 673 726 2028
Size: 327 346 53 1302
Fragments arranged by size:
1302 346 327 53
ApaI G_GGCC'C
Cuts at: 0 242 2028
Size: 242 1786
ApaLI G'TGCA_C
Cuts at: 0 205 2028
Size: 205 1823
AseI AT'TA_AT
Cuts at: 0 908 2028
Size: 908 1120
AvaI C'yCGr_G
Cuts at: 0 86 1247 1385 1601 1941 2028
Size: 86 1161 138 216 340 87
Fragments arranged by size:
1161 340 216 138 87 86
BanI G'GyrC_C
Cuts at: 0 213 1861 2028
Size: 213 1648 167
Fragments arranged by size:
1648 213 167
BanII G_rGCy'C
Cuts at: 0 100 242 515 617 856 961 1523 1576
Size: 100 142 273 102 239 105 562 53
Cuts at: 1576 2028
Size: 452
Fragments arranged by size:
562 452 273 239 142 105 102 100
53
BbeI G_GCGC'C
Cuts at: 0 217 2028
Size: 217 1811
BbsI GAAGACnn'nnnn_
Cuts at: 0 509 1140 1403 2028
Size: 509 631 263 625
Fragments arranged by size:
631 625 509 263
BbvCI CC'TCA_GC
Cuts at: 0 146 2028
Size: 146 1882
BglI GCCn_nnn'nGGC
Cuts at: 0 43 802 2028
Size: 43 759 1226
Fragments arranged by size:
1226 759 43
BglII A'GATC_T
Cuts at: 0 1541 1937 2028
Size: 1541 396 91
Fragments arranged by size:
1541 396 91
BlpI GC'TnA_GC
Cuts at: 0 674 839 997 1647 1828 2028
Size: 674 165 158 650 181 200
Fragments arranged by size:
674 650 200 181 165 158
Bme1580I G_kGCm'C
Cuts at: 0 209 242 1864 2028
Size: 209 33 1622 164
Fragments arranged by size:
1622 209 164 33
BmrI ACTGGGnnnn_n'
Cuts at: 0 214 714 2028
Size: 214 500 1314
Fragments arranged by size:
1314 500 214
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 870 1563 2028
Size: 870 693 465
Fragments arranged by size:
870 693 465
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 349 385 639 1507 1615 1862 2028
Size: 349 36 254 868 108 247 166
Fragments arranged by size:
868 349 254 247 166 108 36
Bpu10I CC'TnA_GC
Cuts at: 0 146 1575 2028
Size: 146 1429 453
Fragments arranged by size:
1429 453 146
BsaI GGTCTCn'nnnn_
Cuts at: 0 739 1591 1838 2028
Size: 739 852 247 190
Fragments arranged by size:
852 739 247 190
BsaBI GATnn'nnATC
Cuts at: 0 586 2028
Size: 586 1442
BsaHI Gr'CG_yC
Cuts at: 0 214 2028
Size: 214 1814
BsaWI w'CCGG_w
Cuts at: 0 28 656 1379 1869 2028
Size: 28 628 723 490 159
Fragments arranged by size:
723 628 490 159 28
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2005 2028
Size: 2005 23
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 163 295 433 636 1403 1578 1903 2028
Size: 163 132 138 203 767 175 325 125
Fragments arranged by size:
767 325 203 175 163 138 132 125
BseYI C'CCAG_C
Cuts at: 0 234 666 1714 2028
Size: 234 432 1048 314
Fragments arranged by size:
1048 432 314 234
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1108 1213 1466 1636 1691 2028
Size: 1108 105 253 170 55 337
Fragments arranged by size:
1108 337 253 170 105 55
BsiHKAI G_wGCw'C
Cuts at: 0 209 401 515 680 692 1523 1576 1880
Size: 209 192 114 165 12 831 53 304
Cuts at: 1880 2028
Size: 148
Fragments arranged by size:
831 304 209 192 165 148 114 53
12
BsmI GAATG_Cn'
Cuts at: 0 546 2028
Size: 546 1482
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2025 2028
Size: 2025 3
Bsp1286I G_dGCh'C
Cuts at: 0 100 209 242 401 515 617 680 692
Size: 100 109 33 159 114 102 63 12
Cuts at: 692 856 961 1523 1576 1864 1880 2028
Size: 164 105 562 53 288 16 148
Fragments arranged by size:
562 288 164 159 148 114 109 105
102 100 63 53 33 16 12
BspEI T'CCGG_A
Cuts at: 0 656 1379 1869 2028
Size: 656 723 490 159
Fragments arranged by size:
723 656 490 159
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1127 1263 1651 1715 2028
Size: 1127 136 388 64 313
Fragments arranged by size:
1127 388 313 136 64
BsrBI CCG'CTC
Cuts at: 0 1959 2028
Size: 1959 69
BsrDI GCAATG_nn'
Cuts at: 0 1089 2028
Size: 1089 939
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1122 2028
Size: 1122 906
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1749 2028
Size: 1749 279
BstYI r'GATC_y
Cuts at: 0 644 1541 1937 2028
Size: 644 897 396 91
Fragments arranged by size:
897 644 396 91
BstZ17I GTA'TAC
Cuts at: 0 1582 2028
Size: 1582 446
Bsu36I CC'TnA_GG
Cuts at: 0 1142 1370 2028
Size: 1142 228 658
Fragments arranged by size:
1142 658 228
BtgI C'CryG_G
Cuts at: 0 289 797 1628 2028
Size: 289 508 831 400
Fragments arranged by size:
831 508 400 289
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1893 2028
Size: 1893 135
BtsI GCAGTG_nn'
Cuts at: 0 103 134 477 1179 1454 2028
Size: 103 31 343 702 275 574
Fragments arranged by size:
702 574 343 275 103 31
DraIII CAC_nnn'GTG
Cuts at: 0 396 2028
Size: 396 1632
EaeI y'GGCC_r
Cuts at: 0 2 297 1064 2028
Size: 2 295 767 964
Fragments arranged by size:
964 767 295 2
EarI CTCTTCn'nnn_
Cuts at: 0 313 1198 2028
Size: 313 885 830
Fragments arranged by size:
885 830 313
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 742 1917 2028
Size: 742 1175 111
Fragments arranged by size:
1175 742 111
EcoICRI GAG'CTC
Cuts at: 0 513 1521 1574 2028
Size: 513 1008 53 454
Fragments arranged by size:
1008 513 454 53
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 349 385 409 639 1507 1615 1862 2028
Size: 349 36 24 230 868 108 247 166
Fragments arranged by size:
868 349 247 230 166 108 36 24
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1564 2028
Size: 1564 464
EcoO109I rG'GnC_Cy
Cuts at: 0 83 238 239 341 662 817 1107 1853
Size: 83 155 1 102 321 155 290 746
Cuts at: 1853 1893 1968 2028
Size: 40 75 60
Fragments arranged by size:
746 321 290 155 155 102 83 75
60 40 1
EcoRV GAT'ATC
Cuts at: 0 1377 2028
Size: 1377 651
FspI TGC'GCA
Cuts at: 0 1081 2028
Size: 1081 947
HaeII r_GCGC'y
Cuts at: 0 146 217 414 2028
Size: 146 71 197 1614
Fragments arranged by size:
1614 197 146 71
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 49 81 2028
Size: 49 32 1947
Fragments arranged by size:
1947 49 32
KasI G'GCGC_C
Cuts at: 0 213 2028
Size: 213 1815
MscI TGG'CCA
Cuts at: 0 1066 2028
Size: 1066 962
MslI CAynn'nnrTG
Cuts at: 0 1630 2028
Size: 1630 398
MspA1I CmG'CkG
Cuts at: 0 269 279 291 673 758 975 1173 1344
Size: 269 10 12 382 85 217 198 171
Cuts at: 1344 1725 2028
Size: 381 303
Fragments arranged by size:
382 381 303 269 217 198 171 85
12 10
NarI GG'CG_CC
Cuts at: 0 214 2028
Size: 214 1814
NcoI C'CATG_G
Cuts at: 0 797 1628 2028
Size: 797 831 400
Fragments arranged by size:
831 797 400
NspI r_CATG'y
Cuts at: 0 1660 1722 1760 1802 2028
Size: 1660 62 38 42 226
Fragments arranged by size:
1660 226 62 42 38
PasI CC'CwG_GG
Cuts at: 0 1857 2028
Size: 1857 171
PciI A'CATG_T
Cuts at: 0 1798 2028
Size: 1798 230
PflMI CCAn_nnn'nTGG
Cuts at: 0 327 1073 2028
Size: 327 746 955
Fragments arranged by size:
955 746 327
PfoI T'CCnGG_A
Cuts at: 0 170 1952 2028
Size: 170 1782 76
Fragments arranged by size:
1782 170 76
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2005 2028
Size: 2005 23
PpuMI rG'GwC_Cy
Cuts at: 0 83 341 662 817 2028
Size: 83 258 321 155 1211
Fragments arranged by size:
1211 321 258 155 83
PshAI GACnn'nnGTC
Cuts at: 0 901 2028
Size: 901 1127
PspOMI G'GGCC_C
Cuts at: 0 238 2028
Size: 238 1790
PstI C_TGCA'G
Cuts at: 0 356 2028
Size: 356 1672
PvuII CAG'CTG
Cuts at: 0 279 673 1173 1344 1725 2028
Size: 279 394 500 171 381 303
Fragments arranged by size:
500 394 381 303 279 171
SacI G_AGCT'C
Cuts at: 0 515 1523 1576 2028
Size: 515 1008 53 452
Fragments arranged by size:
1008 515 452 53
SacII CC_GC'GG
Cuts at: 0 292 2028
Size: 292 1736
SanDI GG'GwC_CC
Cuts at: 0 83 341 2028
Size: 83 258 1687
Fragments arranged by size:
1687 258 83
SfcI C'TryA_G
Cuts at: 0 352 556 2028
Size: 352 204 1472
Fragments arranged by size:
1472 352 204
SfoI GGC'GCC
Cuts at: 0 215 2028
Size: 215 1813
SmaI CCC'GGG
Cuts at: 0 88 1249 1603 2028
Size: 88 1161 354 425
Fragments arranged by size:
1161 425 354 88
SmlI C'TyrA_G
Cuts at: 0 1941 2028
Size: 1941 87
SphI G_CATG'C
Cuts at: 0 1660 1722 2028
Size: 1660 62 306
Fragments arranged by size:
1660 306 62
StuI AGG'CCT
Cuts at: 0 1528 1748 2028
Size: 1528 220 280
Fragments arranged by size:
1528 280 220
StyI C'CwwG_G
Cuts at: 0 368 797 1550 1628 2028
Size: 368 429 753 78 400
Fragments arranged by size:
753 429 400 368 78
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1065 1728 1749 2028
Size: 1065 663 21 279
Fragments arranged by size:
1065 663 279 21
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 727 2028
Size: 727 1301
XhoI C'TCGA_G
Cuts at: 0 1941 2028
Size: 1941 87
XmaI C'CCGG_G
Cuts at: 0 86 1247 1601 2028
Size: 86 1161 354 427
Fragments arranged by size:
1161 427 354 86
Enzymes that do cut:
AarI AccI AcuI AflIII AhdI AleI AlfI AloI
AlwNI ApaI ApaLI AseI AvaI BanI BanII BbeI
BbsI BbvCI BglI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BsaI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspMI
BsrBI BsrDI BstAPI BstXI BstYI BstZ17I Bsu36I BtgI
BtgZI BtsI DraIII EaeI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRV FspI HaeII Hin4I KasI MscI
MslI MspA1I NarI NcoI NspI PasI PciI PflMI
PfoI PpiI PpuMI PshAI PspOMI PstI PvuII SacI
SacII SanDI SfcI SfoI SmaI SmlI SphI StuI
StyI TaqII XcmI XhoI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI ApoI AscI
AsiSI AvrII BaeI BamHI BcgI BciVI BclI BfrBI
BmgBI BmtI BpuEI BsaAI BsiEI BsiWI BspHI BsrFI
BsrGI BssHII BssSI BstBI BstEII ClaI CspCI DraI
DrdI EagI EcoRI FalI FseI FspAI HincII HindIII
HpaI KpnI MfeI MluI MmeI NaeI NdeI NgoMIV
NheI NotI NruI NsiI PacI PmeI PmlI PsiI
PspXI PsrI PvuI RsrII SalI SapI SbfI ScaI
SexAI SfiI SgfI SgrAI SnaBI SpeI SrfI SspI
SwaI TatI TstI Tth111I XbaI XmnI ZraI