(Linear) (Six-base) MAPSORT of: KIEE1569.seq  Check: 2448  from: 1  to: 261



KIEE1569.orf

 With 182 enzymes: SgfI * 

                        July 15, 2010 18:42  ..

AccI GT'mk_AC

Cuts at:      0     132     261 
   Size:        132     129
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     157     259     261 
   Size:        157     102       2

  Fragments arranged by size: 

                 157     102       2
 
BbvCI CC'TCA_GC

Cuts at:      0      65     261 
   Size:         65     196
 
Bpu10I CC'TnA_GC

Cuts at:      0      65     205     261 
   Size:         65     140      56

  Fragments arranged by size: 

                 140      65      56
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     112     261 
   Size:        112     149
 
BsiHKAI G_wGCw'C

Cuts at:      0     211     261 
   Size:        211      50
 
Bsp1286I G_dGCh'C

Cuts at:      0     211     261 
   Size:        211      50
 
BtsI GCAGTG_nn'

Cuts at:      0      17     261 
   Size:         17     244
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     157     259     261 
   Size:        157     102       2

  Fragments arranged by size: 

                 157     102       2
 
HincII GTy'rAC

Cuts at:      0     133     261 
   Size:        133     128
 
MspA1I CmG'CkG

Cuts at:      0      49     261 
   Size:         49     212
 
PasI CC'CwG_GG

Cuts at:      0      54     261 
   Size:         54     207
 
PfoI T'CCnGG_A

Cuts at:      0      80     261 
   Size:         80     181
 
SalI G'TCGA_C

Cuts at:      0     131     261 
   Size:        131     130
 

 Enzymes that do cut:

     AccI     AcuI    BbvCI   Bpu10I    BseRI  BsiHKAI Bsp1286I     BtsI
  Eco57MI   HincII   MspA1I     PasI     PfoI     SalI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI     BbsI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseYI
     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI
    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI
   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI
    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EarI     EciI
  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI    HaeII    Hin4I  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MmeI     MscI     MslI     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI     PciI
    PflMI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII
    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     StyI     SwaI    TaqII     TatI     TstI  Tth111I
     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI