Restriction map for
pFN21AE1567
(Linear) (Six-base) MAPSORT of: KIEE1567.seq Check: 7326 from: 1 to: 1254
KIEE1567.orf
With 182 enzymes: SgfI *
July 15, 2010 18:42 ..
AccI GT'mk_AC
Cuts at: 0 344 611 1254
Size: 344 267 643
Fragments arranged by size:
643 344 267
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 141 1254
Size: 141 1113
AflIII A'CryG_T
Cuts at: 0 149 1254
Size: 149 1105
AleI CACnn'nnGTG
Cuts at: 0 538 1254
Size: 538 716
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 199 409 1254
Size: 199 210 845
Fragments arranged by size:
845 210 199
ApaI G_GGCC'C
Cuts at: 0 741 1254
Size: 741 513
ApaLI G'TGCA_C
Cuts at: 0 384 1254
Size: 384 870
ApoI r'AATT_y
Cuts at: 0 86 502 760 1254
Size: 86 416 258 494
Fragments arranged by size:
494 416 258 86
BamHI G'GATC_C
Cuts at: 0 362 1254
Size: 362 892
BanI G'GyrC_C
Cuts at: 0 1037 1254
Size: 1037 217
BanII G_rGCy'C
Cuts at: 0 215 551 741 1167 1254
Size: 215 336 190 426 87
Fragments arranged by size:
426 336 215 190 87
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 321 355 1254
Size: 321 34 899
Fragments arranged by size:
899 321 34
BclI T'GATC_A
Cuts at: 0 275 1254
Size: 275 979
BglI GCCn_nnn'nGGC
Cuts at: 0 838 1254
Size: 838 416
BlpI GC'TnA_GC
Cuts at: 0 1132 1254
Size: 1132 122
Bme1580I G_kGCm'C
Cuts at: 0 188 388 741 1042 1254
Size: 188 200 353 301 212
Fragments arranged by size:
353 301 212 200 188
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 30 486 1254
Size: 30 456 768
Fragments arranged by size:
768 456 30
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 188 229 1207 1254
Size: 188 41 978 47
Fragments arranged by size:
978 188 47 41
BsaI GGTCTCn'nnnn_
Cuts at: 0 534 1091 1254
Size: 534 557 163
Fragments arranged by size:
557 534 163
BsaAI yAC'GTr
Cuts at: 0 24 183 668 1254
Size: 24 159 485 586
Fragments arranged by size:
586 485 159 24
BsaWI w'CCGG_w
Cuts at: 0 359 1254
Size: 359 895
BseYI C'CCAG_C
Cuts at: 0 59 516 1254
Size: 59 457 738
Fragments arranged by size:
738 457 59
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1132 1254
Size: 1132 122
BsiEI CG_ry'CG
Cuts at: 0 539 1254
Size: 539 715
BsiHKAI G_wGCw'C
Cuts at: 0 388 1254
Size: 388 866
Bsp1286I G_dGCh'C
Cuts at: 0 188 215 388 551 741 1042 1167 1254
Size: 188 27 173 163 190 301 125 87
Fragments arranged by size:
301 190 188 173 163 125 87 27
BsrBI CCG'CTC
Cuts at: 0 465 1254
Size: 465 789
BsrDI GCAATG_nn'
Cuts at: 0 57 1254
Size: 57 1197
BsrGI T'GTAC_A
Cuts at: 0 953 1254
Size: 953 301
BssSI C'ACGA_G
Cuts at: 0 847 1254
Size: 847 407
BstAPI GCAn_nnn'nTGC
Cuts at: 0 56 1254
Size: 56 1198
BstXI CCAn_nnnn'nTGG
Cuts at: 0 421 540 903 1254
Size: 421 119 363 351
Fragments arranged by size:
421 363 351 119
BstYI r'GATC_y
Cuts at: 0 362 378 1254
Size: 362 16 876
Fragments arranged by size:
876 362 16
BtgI C'CryG_G
Cuts at: 0 533 539 860 924 1079 1142 1220 1254
Size: 533 6 321 64 155 63 78 34
Fragments arranged by size:
533 321 155 78 64 63 34 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 978 1134 1254
Size: 978 156 120
Fragments arranged by size:
978 156 120
BtsI GCAGTG_nn'
Cuts at: 0 200 1254
Size: 200 1054
EaeI y'GGCC_r
Cuts at: 0 296 536 806 989 1103 1145 1254
Size: 296 240 270 183 114 42 109
Fragments arranged by size:
296 270 240 183 114 109 42
EagI C'GGCC_G
Cuts at: 0 536 1254
Size: 536 718
EarI CTCTTCn'nnn_
Cuts at: 0 262 1196 1254
Size: 262 934 58
Fragments arranged by size:
934 262 58
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 391 1254
Size: 391 863
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 141 188 229 1207 1254
Size: 141 47 41 978 47
Fragments arranged by size:
978 141 47 47 41
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1105 1254
Size: 1105 149
EcoO109I rG'GnC_Cy
Cuts at: 0 737 738 1254
Size: 737 1 516
Fragments arranged by size:
737 516 1
EcoRI G'AATT_C
Cuts at: 0 86 760 1254
Size: 86 674 494
Fragments arranged by size:
674 494 86
FspI TGC'GCA
Cuts at: 0 235 1254
Size: 235 1019
FspAI rTGC'GCAy
Cuts at: 0 235 1254
Size: 235 1019
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 29 61 841 873 1254
Size: 29 32 780 32 381
Fragments arranged by size:
780 381 32 32 29
HincII GTy'rAC
Cuts at: 0 345 612 984 1254
Size: 345 267 372 270
Fragments arranged by size:
372 345 270 267
MscI TGG'CCA
Cuts at: 0 808 1105 1254
Size: 808 297 149
Fragments arranged by size:
808 297 149
MslI CAynn'nnrTG
Cuts at: 0 538 1254
Size: 538 716
MspA1I CmG'CkG
Cuts at: 0 273 855 1116 1254
Size: 273 582 261 138
Fragments arranged by size:
582 273 261 138
NdeI CA'TA_TG
Cuts at: 0 634 1254
Size: 634 620
NspI r_CATG'y
Cuts at: 0 951 1131 1254
Size: 951 180 123
Fragments arranged by size:
951 180 123
PasI CC'CwG_GG
Cuts at: 0 1090 1254
Size: 1090 164
PfoI T'CCnGG_A
Cuts at: 0 353 1254
Size: 353 901
PmlI CAC'GTG
Cuts at: 0 24 183 1254
Size: 24 159 1071
Fragments arranged by size:
1071 159 24
PshAI GACnn'nnGTC
Cuts at: 0 635 1254
Size: 635 619
PspOMI G'GGCC_C
Cuts at: 0 737 1254
Size: 737 517
PstI C_TGCA'G
Cuts at: 0 398 1254
Size: 398 856
PvuII CAG'CTG
Cuts at: 0 273 855 1254
Size: 273 582 399
Fragments arranged by size:
582 399 273
SalI G'TCGA_C
Cuts at: 0 343 610 1254
Size: 343 267 644
Fragments arranged by size:
644 343 267
SapI GCTCTTCn'nnn_
Cuts at: 0 262 1254
Size: 262 992
SexAI A'CCwGG_T
Cuts at: 0 347 1254
Size: 347 907
SfcI C'TryA_G
Cuts at: 0 246 394 1254
Size: 246 148 860
Fragments arranged by size:
860 246 148
SphI G_CATG'C
Cuts at: 0 1131 1254
Size: 1131 123
StuI AGG'CCT
Cuts at: 0 841 1159 1254
Size: 841 318 95
Fragments arranged by size:
841 318 95
StyI C'CwwG_G
Cuts at: 0 1106 1254
Size: 1106 148
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 278 586 1254
Size: 278 308 668
Fragments arranged by size:
668 308 278
TatI w'GTAC_w
Cuts at: 0 68 932 953 1254
Size: 68 864 21 301
Fragments arranged by size:
864 301 68 21
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1040 1072 1254
Size: 1040 32 182
Fragments arranged by size:
1040 182 32
Tth111I GACn'n_nGTC
Cuts at: 0 349 1254
Size: 349 905
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 540 1254
Size: 540 714
Enzymes that do cut:
AccI AcuI AflIII AleI AlfI ApaI ApaLI ApoI
BamHI BanI BanII BcgI BclI BglI BlpI Bme1580I
BplI BpmI BsaI BsaAI BsaWI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BsrBI BsrDI BsrGI BssSI BstAPI BstXI
BstYI BtgI BtgZI BtsI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI FspI FspAI Hin4I HincII
MscI MslI MspA1I NdeI NspI PasI PfoI PmlI
PshAI PspOMI PstI PvuII SalI SapI SexAI SfcI
SphI StuI StyI TaqII TatI TstI Tth111I XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AloI AlwNI AscI AseI AsiSI AvaI AvrII BaeI
BbeI BbsI BbvCI BciVI BfrBI BglII BmgBI BmrI
BmtI Bpu10I BpuEI BsaBI BsaHI BsaXI BseRI BsiWI
BsmI BsmBI BspEI BspHI BspMI BsrFI BssHII BstBI
BstEII BstZ17I Bsu36I ClaI CspCI DraI DraIII DrdI
EcoICRI EcoRV FalI FseI HaeII HindIII HpaI KasI
KpnI MfeI MluI MmeI NaeI NarI NcoI NgoMIV
NheI NotI NruI NsiI PacI PciI PflMI PmeI
PpiI PpuMI PsiI PspXI PsrI PvuI RsrII SacI
SacII SanDI SbfI ScaI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SrfI SspI SwaI XbaI
XhoI XmaI XmnI ZraI