Restriction map for
pFN21AE1562
(Linear) (Six-base) MAPSORT of: KIEE1562.seq Check: 9745 from: 1 to: 1446
KIEE1562.orf
With 182 enzymes: SgfI *
July 15, 2010 18:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 340 506 1446
Size: 340 166 940
Fragments arranged by size:
940 340 166
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 796 1446
Size: 796 650
AleI CACnn'nnGTG
Cuts at: 0 148 1196 1446
Size: 148 1048 250
Fragments arranged by size:
1048 250 148
AlwNI CAG_nnn'CTG
Cuts at: 0 239 1017 1035 1126 1146 1446
Size: 239 778 18 91 20 300
Fragments arranged by size:
778 300 239 91 20 18
ApaI G_GGCC'C
Cuts at: 0 514 1446
Size: 514 932
ApoI r'AATT_y
Cuts at: 0 1306 1446
Size: 1306 140
AvaI C'yCGr_G
Cuts at: 0 766 909 1446
Size: 766 143 537
Fragments arranged by size:
766 537 143
BanI G'GyrC_C
Cuts at: 0 298 1446
Size: 298 1148
BanII G_rGCy'C
Cuts at: 0 514 965 1446
Size: 514 451 481
Fragments arranged by size:
514 481 451
BbsI GAAGACnn'nnnn_
Cuts at: 0 1385 1446
Size: 1385 61
BbvCI CC'TCA_GC
Cuts at: 0 923 1446
Size: 923 523
BlpI GC'TnA_GC
Cuts at: 0 959 1446
Size: 959 487
Bme1580I G_kGCm'C
Cuts at: 0 514 1446
Size: 514 932
BmtI G_CTAG'C
Cuts at: 0 433 1446
Size: 433 1013
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 847 1446
Size: 847 599
Bpu10I CC'TnA_GC
Cuts at: 0 923 1010 1446
Size: 923 87 436
Fragments arranged by size:
923 436 87
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 142 1446
Size: 142 1304
BseYI C'CCAG_C
Cuts at: 0 746 1228 1446
Size: 746 482 218
Fragments arranged by size:
746 482 218
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 225 1446
Size: 225 1221
BsiHKAI G_wGCw'C
Cuts at: 0 965 1446
Size: 965 481
BsmBI CGTCTCn'nnnn_
Cuts at: 0 14 1429 1446
Size: 14 1415 17
Fragments arranged by size:
1415 17 14
Bsp1286I G_dGCh'C
Cuts at: 0 514 965 1446
Size: 514 451 481
Fragments arranged by size:
514 481 451
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 340 506 1154 1446
Size: 340 166 648 292
Fragments arranged by size:
648 340 292 166
BsrBI CCG'CTC
Cuts at: 0 206 1241 1446
Size: 206 1035 205
Fragments arranged by size:
1035 206 205
BsrDI GCAATG_nn'
Cuts at: 0 287 1002 1446
Size: 287 715 444
Fragments arranged by size:
715 444 287
BsrFI r'CCGG_y
Cuts at: 0 602 1446
Size: 602 844
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1146 1446
Size: 1146 300
BstXI CCAn_nnnn'nTGG
Cuts at: 0 462 1446
Size: 462 984
BstYI r'GATC_y
Cuts at: 0 63 680 1446
Size: 63 617 766
Fragments arranged by size:
766 617 63
Bsu36I CC'TnA_GG
Cuts at: 0 59 1446
Size: 59 1387
BtgI C'CryG_G
Cuts at: 0 902 1446
Size: 902 544
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 120 1242 1446
Size: 120 1122 204
Fragments arranged by size:
1122 204 120
BtsI GCAGTG_nn'
Cuts at: 0 234 308 1218 1446
Size: 234 74 910 228
Fragments arranged by size:
910 234 228 74
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 129 164 377 412 1446
Size: 129 35 213 35 1034
Fragments arranged by size:
1034 213 129 35 35
EarI CTCTTCn'nnn_
Cuts at: 0 772 1157 1446
Size: 772 385 289
Fragments arranged by size:
772 385 289
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 621 1233 1446
Size: 621 612 213
Fragments arranged by size:
621 612 213
EcoICRI GAG'CTC
Cuts at: 0 963 1446
Size: 963 483
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 796 847 1446
Size: 796 51 599
Fragments arranged by size:
796 599 51
EcoNI CCTnn'n_nnAGG
Cuts at: 0 410 1446
Size: 410 1036
EcoO109I rG'GnC_Cy
Cuts at: 0 416 510 511 533 1014 1446
Size: 416 94 1 22 481 432
Fragments arranged by size:
481 432 416 94 22 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 699 832 1446
Size: 699 133 614
Fragments arranged by size:
699 614 133
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1316 1348 1446
Size: 1316 32 98
Fragments arranged by size:
1316 98 32
HincII GTy'rAC
Cuts at: 0 354 1446
Size: 354 1092
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 78 1446
Size: 78 1368
MslI CAynn'nnrTG
Cuts at: 0 148 1196 1282 1446
Size: 148 1048 86 164
Fragments arranged by size:
1048 164 148 86
MspA1I CmG'CkG
Cuts at: 0 194 236 295 904 1032 1446
Size: 194 42 59 609 128 414
Fragments arranged by size:
609 414 194 128 59 42
NaeI GCC'GGC
Cuts at: 0 604 1446
Size: 604 842
NgoMIV G'CCGG_C
Cuts at: 0 602 1446
Size: 602 844
NheI G'CTAG_C
Cuts at: 0 429 1446
Size: 429 1017
PflMI CCAn_nnn'nTGG
Cuts at: 0 391 1446
Size: 391 1055
PpuMI rG'GwC_Cy
Cuts at: 0 416 1014 1446
Size: 416 598 432
Fragments arranged by size:
598 432 416
PsiI TTA'TAA
Cuts at: 0 1304 1446
Size: 1304 142
PspOMI G'GGCC_C
Cuts at: 0 510 1446
Size: 510 936
PspXI vC'TCGA_Gb
Cuts at: 0 766 909 1446
Size: 766 143 537
Fragments arranged by size:
766 537 143
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1030 1062 1446
Size: 1030 32 384
Fragments arranged by size:
1030 384 32
PstI C_TGCA'G
Cuts at: 0 532 617 1446
Size: 532 85 829
Fragments arranged by size:
829 532 85
PvuII CAG'CTG
Cuts at: 0 236 1446
Size: 236 1210
SacI G_AGCT'C
Cuts at: 0 965 1446
Size: 965 481
SacII CC_GC'GG
Cuts at: 0 905 1446
Size: 905 541
SanDI GG'GwC_CC
Cuts at: 0 416 1446
Size: 416 1030
ScaI AGT'ACT
Cuts at: 0 1156 1446
Size: 1156 290
SfcI C'TryA_G
Cuts at: 0 528 613 651 1446
Size: 528 85 38 795
Fragments arranged by size:
795 528 85 38
SmlI C'TyrA_G
Cuts at: 0 157 766 909 1446
Size: 157 609 143 537
Fragments arranged by size:
609 537 157 143
StuI AGG'CCT
Cuts at: 0 1097 1446
Size: 1097 349
StyI C'CwwG_G
Cuts at: 0 93 564 990 1446
Size: 93 471 426 456
Fragments arranged by size:
471 456 426 93
TatI w'GTAC_w
Cuts at: 0 545 1154 1446
Size: 545 609 292
Fragments arranged by size:
609 545 292
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1056 1088 1391 1423 1446
Size: 1056 32 303 32 23
Fragments arranged by size:
1056 303 32 32 23
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1225 1446
Size: 1225 221
XhoI C'TCGA_G
Cuts at: 0 766 909 1446
Size: 766 143 537
Fragments arranged by size:
766 537 143
Enzymes that do cut:
AarI AcuI AleI AlwNI ApaI ApoI AvaI BanI
BanII BbsI BbvCI BlpI Bme1580I BmtI BpmI Bpu10I
BpuEI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspMI BsrBI
BsrDI BsrFI BstAPI BstXI BstYI Bsu36I BtgI BtgZI
BtsI CspCI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
FalI Hin4I HincII MmeI MslI MspA1I NaeI NgoMIV
NheI PflMI PpuMI PsiI PspOMI PspXI PsrI PstI
PvuII SacI SacII SanDI ScaI SfcI SmlI StuI
StyI TatI TstI XcmI XhoI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AlfI AloI ApaLI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BcgI BciVI BclI BfrBI BglI
BglII BmgBI BmrI BplI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BsiEI BsiWI BsmI BspEI BspHI
BsrGI BssHII BssSI BstBI BstEII BstZ17I ClaI DraI
DraIII DrdI EaeI EagI EcoRI EcoRV FseI FspI
FspAI HaeII HindIII HpaI KasI KpnI MfeI MluI
MscI NarI NcoI NdeI NotI NruI NsiI NspI
PacI PasI PciI PfoI PmeI PmlI PpiI PshAI
PvuI RsrII SalI SapI SbfI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
SwaI TaqII Tth111I XbaI XmaI XmnI ZraI