Restriction map for
pFN21AE1558
(Linear) (Six-base) MAPSORT of: KIEE1558.seq Check: 1374 from: 1 to: 1080
KIEE1558.orf
With 182 enzymes: SgfI *
July 15, 2010 18:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 27 1080
Size: 27 1053
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 784 1080
Size: 784 296
AleI CACnn'nnGTG
Cuts at: 0 894 1080
Size: 894 186
AlwNI CAG_nnn'CTG
Cuts at: 0 464 1080
Size: 464 616
ApoI r'AATT_y
Cuts at: 0 770 1080
Size: 770 310
AseI AT'TA_AT
Cuts at: 0 1019 1080
Size: 1019 61
AvaI C'yCGr_G
Cuts at: 0 98 1080
Size: 98 982
BamHI G'GATC_C
Cuts at: 0 1004 1080
Size: 1004 76
BanI G'GyrC_C
Cuts at: 0 358 1080
Size: 358 722
BanII G_rGCy'C
Cuts at: 0 430 719 1058 1080
Size: 430 289 339 22
Fragments arranged by size:
430 339 289 22
BbeI G_GCGC'C
Cuts at: 0 362 1080
Size: 362 718
BbsI GAAGACnn'nnnn_
Cuts at: 0 168 210 1080
Size: 168 42 870
Fragments arranged by size:
870 168 42
BbvCI CC'TCA_GC
Cuts at: 0 986 1080
Size: 986 94
BclI T'GATC_A
Cuts at: 0 1029 1080
Size: 1029 51
BglI GCCn_nnn'nGGC
Cuts at: 0 289 1080
Size: 289 791
BlpI GC'TnA_GC
Cuts at: 0 389 1080
Size: 389 691
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 651 1080
Size: 651 429
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 177 1080
Size: 177 903
Bpu10I CC'TnA_GC
Cuts at: 0 986 1080
Size: 986 94
BsaI GGTCTCn'nnnn_
Cuts at: 0 118 1080
Size: 118 962
BsaHI Gr'CG_yC
Cuts at: 0 359 1080
Size: 359 721
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 70 100 1080
Size: 70 30 980
Fragments arranged by size:
980 70 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 91 148 640 1080
Size: 91 57 492 440
Fragments arranged by size:
492 440 91 57
BseYI C'CCAG_C
Cuts at: 0 676 1080
Size: 676 404
BsiHKAI G_wGCw'C
Cuts at: 0 719 1058 1080
Size: 719 339 22
Fragments arranged by size:
719 339 22
BsmBI CGTCTCn'nnnn_
Cuts at: 0 245 1080
Size: 245 835
Bsp1286I G_dGCh'C
Cuts at: 0 430 719 1058 1080
Size: 430 289 339 22
Fragments arranged by size:
430 339 289 22
BspHI T'CATG_A
Cuts at: 0 651 1080
Size: 651 429
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 27 1080
Size: 27 1053
BsrDI GCAATG_nn'
Cuts at: 0 1032 1080
Size: 1032 48
BstAPI GCAn_nnn'nTGC
Cuts at: 0 298 1080
Size: 298 782
BstXI CCAn_nnnn'nTGG
Cuts at: 0 540 1080
Size: 540 540
BstYI r'GATC_y
Cuts at: 0 1004 1080
Size: 1004 76
BtsI GCAGTG_nn'
Cuts at: 0 232 308 1080
Size: 232 76 772
Fragments arranged by size:
772 232 76
DraI TTT'AAA
Cuts at: 0 944 1080
Size: 944 136
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 88 1080
Size: 88 992
EcoICRI GAG'CTC
Cuts at: 0 717 1056 1080
Size: 717 339 24
Fragments arranged by size:
717 339 24
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 177 784 1080
Size: 177 607 296
Fragments arranged by size:
607 296 177
HaeII r_GCGC'y
Cuts at: 0 362 1080
Size: 362 718
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 92 124 586 618 870 902 1080
Size: 92 32 462 32 252 32 178
Fragments arranged by size:
462 252 178 92 32 32 32
KasI G'GCGC_C
Cuts at: 0 358 1080
Size: 358 722
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 442 982 1080
Size: 442 540 98
Fragments arranged by size:
540 442 98
MslI CAynn'nnrTG
Cuts at: 0 650 656 894 1080
Size: 650 6 238 186
Fragments arranged by size:
650 238 186 6
MspA1I CmG'CkG
Cuts at: 0 178 819 1080
Size: 178 641 261
Fragments arranged by size:
641 261 178
NarI GG'CG_CC
Cuts at: 0 359 1080
Size: 359 721
PshAI GACnn'nnGTC
Cuts at: 0 214 1080
Size: 214 866
PspXI vC'TCGA_Gb
Cuts at: 0 98 1080
Size: 98 982
PstI C_TGCA'G
Cuts at: 0 24 516 1080
Size: 24 492 564
Fragments arranged by size:
564 492 24
PvuII CAG'CTG
Cuts at: 0 178 819 1080
Size: 178 641 261
Fragments arranged by size:
641 261 178
SacI G_AGCT'C
Cuts at: 0 719 1058 1080
Size: 719 339 22
Fragments arranged by size:
719 339 22
ScaI AGT'ACT
Cuts at: 0 404 1080
Size: 404 676
SfcI C'TryA_G
Cuts at: 0 20 512 1080
Size: 20 492 568
Fragments arranged by size:
568 492 20
SfoI GGC'GCC
Cuts at: 0 360 1080
Size: 360 720
SmlI C'TyrA_G
Cuts at: 0 98 1080
Size: 98 982
TatI w'GTAC_w
Cuts at: 0 305 402 1080
Size: 305 97 678
Fragments arranged by size:
678 305 97
Tth111I GACn'n_nGTC
Cuts at: 0 186 1080
Size: 186 894
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 998 1080
Size: 998 82
XhoI C'TCGA_G
Cuts at: 0 98 1080
Size: 98 982
XmnI GAAnn'nnTTC
Cuts at: 0 918 1080
Size: 918 162
Enzymes that do cut:
AarI AcuI AleI AlwNI ApoI AseI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BclI BglI BlpI
BplI BpmI Bpu10I BsaI BsaHI BsaXI BseRI BseYI
BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrDI BstAPI BstXI
BstYI BtsI DraI EciI EcoICRI Eco57MI HaeII Hin4I
KasI MmeI MslI MspA1I NarI PshAI PspXI PstI
PvuII SacI ScaI SfcI SfoI SmlI TatI Tth111I
XcmI XhoI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AlfI AloI ApaI ApaLI AscI AsiSI AvrII
BaeI BcgI BciVI BfrBI BglII Bme1580I BmgBI BmrI
BmtI BpuEI BsaAI BsaBI BsaWI BsgI BsiEI BsiWI
BsmI BspEI BsrBI BsrFI BsrGI BssHII BssSI BstBI
BstEII BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DraIII
DrdI EaeI EagI EarI EcoNI EcoO109I EcoRI EcoRV
FalI FseI FspI FspAI HincII HindIII HpaI KpnI
MfeI MluI MscI NaeI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI PacI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PsiI PspOMI PsrI
PvuI RsrII SacII SalI SanDI SapI SbfI SexAI
SfiI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI StuI StyI SwaI TaqII TstI XbaI XmaI
ZraI