Restriction map for
pFN21AE1557
(Linear) (Six-base) MAPSORT of: KIEE1557.seq Check: 7477 from: 1 to: 984
KIEE1557.orf
With 182 enzymes: SgfI *
July 15, 2010 18:42 ..
Acc65I G'GTAC_C
Cuts at: 0 821 984
Size: 821 163
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 834 941 984
Size: 834 107 43
Fragments arranged by size:
834 107 43
AflIII A'CryG_T
Cuts at: 0 107 984
Size: 107 877
AgeI A'CCGG_T
Cuts at: 0 4 984
Size: 4 980
AleI CACnn'nnGTG
Cuts at: 0 112 984
Size: 112 872
AlwNI CAG_nnn'CTG
Cuts at: 0 87 436 984
Size: 87 349 548
Fragments arranged by size:
548 349 87
AvaI C'yCGr_G
Cuts at: 0 37 984
Size: 37 947
BamHI G'GATC_C
Cuts at: 0 369 783 984
Size: 369 414 201
Fragments arranged by size:
414 369 201
BanI G'GyrC_C
Cuts at: 0 169 821 984
Size: 169 652 163
Fragments arranged by size:
652 169 163
BbeI G_GCGC'C
Cuts at: 0 173 984
Size: 173 811
BclI T'GATC_A
Cuts at: 0 258 984
Size: 258 726
BfrBI ATG'CAT
Cuts at: 0 243 984
Size: 243 741
BmgBI CAC'GTC
Cuts at: 0 110 984
Size: 110 874
BmrI ACTGGGnnnn_n'
Cuts at: 0 75 700 984
Size: 75 625 284
Fragments arranged by size:
625 284 75
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 231 458 984
Size: 231 227 526
Fragments arranged by size:
526 231 227
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 136 984
Size: 136 848
BsaI GGTCTCn'nnnn_
Cuts at: 0 434 984
Size: 434 550
BsaAI yAC'GTr
Cuts at: 0 507 984
Size: 507 477
BsaHI Gr'CG_yC
Cuts at: 0 170 984
Size: 170 814
BsaWI w'CCGG_w
Cuts at: 0 4 984
Size: 4 980
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 42 87 984
Size: 42 45 897
Fragments arranged by size:
897 45 42
BseYI C'CCAG_C
Cuts at: 0 391 984
Size: 391 593
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 529 984
Size: 529 455
BsmBI CGTCTCn'nnnn_
Cuts at: 0 124 984
Size: 124 860
BsrFI r'CCGG_y
Cuts at: 0 4 132 166 443 984
Size: 4 128 34 277 541
Fragments arranged by size:
541 277 128 34 4
BssSI C'ACGA_G
Cuts at: 0 828 984
Size: 828 156
BstAPI GCAn_nnn'nTGC
Cuts at: 0 844 984
Size: 844 140
BstYI r'GATC_y
Cuts at: 0 369 783 984
Size: 369 414 201
Fragments arranged by size:
414 369 201
Bsu36I CC'TnA_GG
Cuts at: 0 635 984
Size: 635 349
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 819 984
Size: 819 165
EaeI y'GGCC_r
Cuts at: 0 134 984
Size: 134 850
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 231 458 834 941 984
Size: 231 227 376 107 43
Fragments arranged by size:
376 231 227 107 43
EcoO109I rG'GnC_Cy
Cuts at: 0 278 395 984
Size: 278 117 589
Fragments arranged by size:
589 278 117
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 286 675 984
Size: 286 389 309
Fragments arranged by size:
389 309 286
HaeII r_GCGC'y
Cuts at: 0 173 984
Size: 173 811
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 34 66 387 419 984
Size: 34 32 321 32 565
Fragments arranged by size:
565 321 34 32 32
KasI G'GCGC_C
Cuts at: 0 169 984
Size: 169 815
KpnI G_GTAC'C
Cuts at: 0 825 984
Size: 825 159
MfeI C'AATT_G
Cuts at: 0 232 984
Size: 232 752
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 223 984
Size: 223 761
MslI CAynn'nnrTG
Cuts at: 0 112 984
Size: 112 872
NaeI GCC'GGC
Cuts at: 0 134 984
Size: 134 850
NarI GG'CG_CC
Cuts at: 0 170 984
Size: 170 814
NgoMIV G'CCGG_C
Cuts at: 0 132 984
Size: 132 852
NsiI A_TGCA'T
Cuts at: 0 245 984
Size: 245 739
NspI r_CATG'y
Cuts at: 0 208 984
Size: 208 776
PfoI T'CCnGG_A
Cuts at: 0 176 984
Size: 176 808
PpuMI rG'GwC_Cy
Cuts at: 0 278 395 984
Size: 278 117 589
Fragments arranged by size:
589 278 117
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 487 519 984
Size: 487 32 465
Fragments arranged by size:
487 465 32
SanDI GG'GwC_CC
Cuts at: 0 278 984
Size: 278 706
SfoI GGC'GCC
Cuts at: 0 171 984
Size: 171 813
SgrAI Cr'CCGG_yG
Cuts at: 0 166 984
Size: 166 818
SmlI C'TyrA_G
Cuts at: 0 151 984
Size: 151 833
SphI G_CATG'C
Cuts at: 0 208 984
Size: 208 776
SspI AAT'ATT
Cuts at: 0 690 777 984
Size: 690 87 207
Fragments arranged by size:
690 207 87
TatI w'GTAC_w
Cuts at: 0 979 984
Size: 979 5
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 900 932 984
Size: 900 32 52
Fragments arranged by size:
900 52 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 206 984
Size: 206 778
Enzymes that do cut:
Acc65I AcuI AflIII AgeI AleI AlwNI AvaI BamHI
BanI BbeI BclI BfrBI BmgBI BmrI BpmI BpuEI
BsaI BsaAI BsaHI BsaWI BseRI BseYI BsgI BsmBI
BsrFI BssSI BstAPI BstYI Bsu36I BtgZI EaeI Eco57MI
EcoO109I FalI HaeII Hin4I KasI KpnI MfeI MmeI
MslI NaeI NarI NgoMIV NsiI NspI PfoI PpuMI
PsrI SanDI SfoI SgrAI SmlI SphI SspI TatI
TstI XcmI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AhdI AlfI
AloI ApaI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BanII BbsI BbvCI BcgI BciVI BglI BglII
BlpI Bme1580I BmtI BplI Bpu10I BsaBI BsaXI BsiEI
BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrGI BssHII BstBI BstEII BstXI BstZ17I BtgI
BtsI ClaI CspCI DraI DraIII DrdI EagI EarI
EciI EcoICRI EcoNI EcoRI EcoRV FseI FspI FspAI
HincII HindIII HpaI MluI MscI MspA1I NcoI NdeI
NheI NotI NruI PacI PasI PciI PflMI PmeI
PmlI PpiI PshAI PsiI PspOMI PspXI PstI PvuI
PvuII RsrII SacI SacII SalI SapI SbfI ScaI
SexAI SfcI SfiI SgfI SmaI SnaBI SpeI SrfI
StuI StyI SwaI TaqII Tth111I XbaI XhoI XmaI
XmnI ZraI